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3D Cell Culture Without the Fancy Glasses

Mary Strother, Ph.D.
Cell culture is the traditional tool for investigating complex scientific questions related to cell behavior. Growing cells in an environment that more closely resembles the in vivo growth conditions is becoming more commonly accepted as critical to impacting cell behavior.

This webinar will explore many of the cell growth conditions currently in use to achieve 3D cell culture. Some topics covered include: Cell/cell interaction in sphere vs. monolayer; multi-well spheroid culture; transwell to tease out signaling effects; 2D surface effects on cells - micro patterned surfaces.
Jun 23 2011
58 mins
3D Cell Culture Without the Fancy Glasses
life sciences cell culture transwell
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  • Tips and Techniques for Primary Cell Culture Recorded: Apr 10 2012 60 mins
    The use of primary cells as a model system is expanding and so are the protocols in which they are used. Primary cells are extremely valuable to a researcher due to their ability to closely mimic an in vivo-like state and generate more physiologically relevant data. This seminar will cover methods to isolate primary cells from tissues, the importance of choosing the correct dissociation method for your application, and troubleshooting techniques to culture healthier primary cells.
  • Human Cell Line Misidentification: The Beginning of the End Recorded: Mar 20 2012 61 mins
    The misidentification of human cell lines in research labs is a significant problem that may be costing the scientific community millions of dollars annually. Authenticating human cell lines is a crucial, yet underappreciated, quality control step for researchers performing cell biology, genetic mapping, gene expression, and gene therapy research. The validity of conclusions drawn from research data is dependent on consistent and unequivocal verification of cell line identity and function. Over the past 50 years numerous human cell lines have been shown to be misidentified due, in part, to poor cell-culture technique and inadequate authentication protocols. Technological advances have given rise to improved authentication capabilities.

    This one-hour presentation will provide an overview of the current technologies used to authenticate human and animal cell lines.
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    This one-hour webinar will discuss basic practices for combating contamination in tissue culture laboratories. It will include a review of common laboratory contamination sources as well as how to choose and use an appropriate disinfectant. Basic clean laboratory practices as they apply to tissue culture activities will be discussed. The use and cleaning of biosafety cabinets and incubators and the use of appropriate personal protective equipment will be covered. A basic checklist for investigating contamination issues will also be provided. This webinar will be interactive, and the audience will have the ability to ask questions, as time allows.

    This course has been pre-approved for 0.125 ABSA Certification Maintenance Points.
  • Primer-dimer Formation: The Problem and the Solution Recorded: Jan 10 2012 57 mins
    Primer-dimer formation has been always considered as the nemesis not only on sequencing but also on real-time PCR. The presence of primer-dimers in real-time PCR can elevate the background signal altering the Ct value and thus providing misleading expression levels.

    In this seminar we will discuss the primer-dimer formation effect on various genomic downstream applications, the approaches to reduce the potential of its formation, and the pros and cons of different techniques of removing primer-dimers post-PCR.
  • Achieving More In Vivo-like Cell Culture Growth on Permeable Supports Recorded: Dec 13 2011 44 mins
    Permeable support systems are a unique tool which allow users to culture their cells and assay in a more in vivo way than traditional cell culture plates. Corning’s Transwells can be used in a variety of applications to help researchers study transport and other metabolic activities as well as cell migration and tissue remodeling.

    This seminar will talk about some of the major applications for Transwells as well as offer some tips and tricks for handling permeable support systems.
Training for Life Science Researchers
The Corning Scientific Seminar Series is a series of free, online technical presentations that provide novel tips, best practices and proven techniques to help advance your research. Delivered by scientists to scientists, these one-hour broadcasts offer useful information and tips for lab technicians and researchers.
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  • Presented by: Mary Strother, Ph.D.
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