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Cell Culture Contamination Part 3

Hilary Sherman
Recent studies have shown that at least 20% of the animal cell cultures currently in use in the US are contaminated by either microorganisms or other cell lines! This three part live, on-line seminar series will review the scope of this major problem and examine some of its causes and techniques for avoiding it.  It will also explore some key, easy to employ strategies for preventing these losses by careful culture management.
Mar 3 2011
41 mins
Cell Culture Contamination Part 3
cell culture life sciences
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Corning Scientific Seminar Series

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  • Defined,Animal-free Synthetic Surfaces for Culture of Cells in Clinical Research Jun 19 2013 3:00 pm UTC 60 mins
    Expansion of stem cells and some primary cells requires either animal-origin components in culture media or coating of the culture vessels with human or animal-derived extracellular matrix (ECM) protein. Growing concerns about introducing human and animal-derived pathogens into the culture necessitate the need for animal- free (xeno-free and human-origin components-free) culture environment. Herein, we present defined and synthetic surfaces for cell culture. Corning® PureCoat™ ECM Mimetic cultureware collagen I peptide and fibronectin peptide support culture of cell types requiring collagen I or fibronectin protein -coated surfaces. Corning Synthemax® surface consists of a vitronectin peptide and supports undifferentiated expansion of human pluripotent stem cells (hPSC) and neural progenitor cells (NPCs). ECM Mimetic and Synthemax cultureware are pre-coated, synthetic, animal-free, and room temperature stable. Synthemax-SC is available for self-coating.

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  • Tips and Techniques for Enhancing Your Cell Culture Recorded: May 14 2013 61 mins
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    Culturing and producing healthy cells takes more than good sterile technique. Having a clear understanding of a cell culture’s requirements and history is a must. This knowledge is required to facilitate the development of the necessary conditions that will provide an environment for the cell culture to thrive . This webinar will cover the following topics:
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    • Understand the impact of stress on cultures and provide some ideas on to reduce stressful conditions
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    Transfection and transduction of eukaryotic cells are powerful techniques employed to manipulate and study gene expression. Reports have indicated that the use of viruses for the delivery of transgenes is one of the most commonly used tools in research. Additionally, virus transductions have been recently used at both the preclinical and clinical stages in cell therapy as well as with vaccine production leading to an increase in demand to produce more virus as efficiently as possible. This webinar will focus on Adenovirus and Lentivirus vector production scale up and will include optimizations techniques that may improve viral yields.
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    In vitro cell-based assays have become a mainstay in many laboratories. The live cell provides a model far superior to that using purified components to build an assay.. Cell-based assays present particular challenges for the experimenter, however, primarily with maintaining cellular responses in microplates that reflect what occurs naturally (i.e. in tissue in a living organism). In this webinar, we will provide helpful advice and insights for setting up and optimizing cell-based assays using automation.

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  • Understanding the How’s and Why’s of Cell Culture Contamination and Ways to Avoi Recorded: Oct 23 2012 59 mins
    Contamination of cell cultures, whether from bacteria, fungus, virus or other cell line is of critical importance to laboratory personnel. Studies have shown that at least 20% of the animal cultures in existence today are contaminated. Cell line contamination can result in decreased productivity, loss of critical study tools, impact data quality and reduce bioprocessing capacity. In hopes of helping to reduce this common problem, this tutorial will focus on:
    • Understanding the nature of contamination and its consequences.
    • Understanding the major sources and causes of cell culture contamination.
    • Discussing aseptic technique and its use in reducing day-to-day contamination.
    • A discussion on the use of antibiotics and their potential hidden dangers.
    • Simple methods to monitor for contamination and the importance in the use of cryopreserved cell lines.
  • An Introduction To Cell Culture Recorded: Sep 4 2012 63 mins
    This online event will introduce the history, theory, basic techniques and potential pit-falls of mammalian cell culture. It is designed for the novice or someone interested in updating their techniques or knowledge in the field. Topics to be discussed include:
    • The importance of cell culture
    • The history and it’s impact on today’s science
    • What do I need to set up a cell culture laboratory?
    • What are the practical theories of cell culture and how can I use them in my laboratory?
    • What are the issues I should be concerned about when performing mammalian cell culture?
  • Theory and Practice of Cell Culture Scale-up: A Beginner's Guide for Scaling Up Recorded: Jul 10 2012 59 mins
    In the past several decades, the usage of mammalian cell culture processes have allowed for better understanding of basic research and manufacturing of higher quality products compared to those from bacterial work. Although much progress has been developed throughout the years, limitations and challenges during optimization of such processes in scale-up of mammalian cell cultures can be encountered.

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  • siRNA Screening to Identify Novel Modulators of Aβ Homeostasis Recorded: Jun 12 2012 59 mins
    Alzheimer’s disease (AD) is the most common form of dementia and is an incurable, degenerative and terminal disease that is generally diagnosed in people over 65. The original amyloid hypothesis postulated that amyloid beta (Aβ) deposits are the fundamental cause of the disease. Aβ is derived from cleavage of amyloid precursor protein (APP) resulting in various peptide lengths (38-43 amino acids). Although the role of amyloidosis in AD has evolved over the years, aberrant production of Aβ peptides and their aggregation is still believed to play a key pathological role in AD, and has continuously been the focus of AD research.

    This seminar will review how an Amgen laboratory identified novel modulators of Aβ Homeostasis via high-throughput siRNA screening. It will review how this lab performed a primary screen on a human CNS-derived cell line amenable to siRNA transfection. It will describe the results of the initial screen and the counter screen performed to filter out genes involved in cell viability. The webinar will conclude by describing the proteins identified with putative novel roles in Aβ processing and the lab’s next steps in further evaluating the function of these proteins via organotypic cultures, RNAi studies, gene knockout studies, and pharmacological tools.
  • Cell Culture Media: What Is In That Bottle? Recorded: May 15 2012 61 mins
    Achieving proper and consistent cellular growth in vitro is paramount to successful and reproducible research. Environmental factors play a large role and can influence the outcome of the cells’ response to stimuli. Classical mammalian cell culture media formulations are very diverse in both the number and the concentration of constituents. Each medium was designed for specific cell types and culturing conditions.

    This seminar will cover some of the key basic components of various cell mediums, their metabolic breakdown, and how users can select the correct conditions for optimized growth and productivity.
  • Tips and Techniques for Primary Cell Culture Recorded: Apr 10 2012 60 mins
    The use of primary cells as a model system is expanding and so are the protocols in which they are used. Primary cells are extremely valuable to a researcher due to their ability to closely mimic an in vivo-like state and generate more physiologically relevant data. This seminar will cover methods to isolate primary cells from tissues, the importance of choosing the correct dissociation method for your application, and troubleshooting techniques to culture healthier primary cells.
  • Human Cell Line Misidentification: The Beginning of the End Recorded: Mar 20 2012 61 mins
    The misidentification of human cell lines in research labs is a significant problem that may be costing the scientific community millions of dollars annually. Authenticating human cell lines is a crucial, yet underappreciated, quality control step for researchers performing cell biology, genetic mapping, gene expression, and gene therapy research. The validity of conclusions drawn from research data is dependent on consistent and unequivocal verification of cell line identity and function. Over the past 50 years numerous human cell lines have been shown to be misidentified due, in part, to poor cell-culture technique and inadequate authentication protocols. Technological advances have given rise to improved authentication capabilities.

    This one-hour presentation will provide an overview of the current technologies used to authenticate human and animal cell lines.
  • Basic Disinfection Practices for Tissue Culture Laboratories Recorded: Feb 2 2012 76 mins
    This one-hour webinar will discuss basic practices for combating contamination in tissue culture laboratories. It will include a review of common laboratory contamination sources as well as how to choose and use an appropriate disinfectant. Basic clean laboratory practices as they apply to tissue culture activities will be discussed. The use and cleaning of biosafety cabinets and incubators and the use of appropriate personal protective equipment will be covered. A basic checklist for investigating contamination issues will also be provided. This webinar will be interactive, and the audience will have the ability to ask questions, as time allows.

    This course has been pre-approved for 0.125 ABSA Certification Maintenance Points.
  • Primer-dimer Formation: The Problem and the Solution Recorded: Jan 10 2012 57 mins
    Primer-dimer formation has been always considered as the nemesis not only on sequencing but also on real-time PCR. The presence of primer-dimers in real-time PCR can elevate the background signal altering the Ct value and thus providing misleading expression levels.

    In this seminar we will discuss the primer-dimer formation effect on various genomic downstream applications, the approaches to reduce the potential of its formation, and the pros and cons of different techniques of removing primer-dimers post-PCR.
  • Achieving More In Vivo-like Cell Culture Growth on Permeable Supports Recorded: Dec 13 2011 44 mins
    Permeable support systems are a unique tool which allow users to culture their cells and assay in a more in vivo way than traditional cell culture plates. Corning’s Transwells can be used in a variety of applications to help researchers study transport and other metabolic activities as well as cell migration and tissue remodeling.

    This seminar will talk about some of the major applications for Transwells as well as offer some tips and tricks for handling permeable support systems.
Training for Life Science Researchers
The Corning Scientific Seminar Series is a series of free, online technical presentations that provide novel tips, best practices and proven techniques to help advance your research. Delivered by scientists to scientists, these one-hour broadcasts offer useful information and tips for lab technicians and researchers.
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  • Title: Cell Culture Contamination Part 3
  • Live at: Mar 3 2011 7:25 pm
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