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Merck KGaA, Darmstadt, Germany Webinars

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  • Lighten Up!  Long-term imaging with ultra-bright, organic AIE cell trackers
    Lighten Up! Long-term imaging with ultra-bright, organic AIE cell trackers Liu Bin, PhD,Department Head - Department of Chemical and Biomolecular Engineering, National University of Singapore Recorded: Feb 22 2018 31 mins
    With the recent discovery of a special class of organic compounds with aggregation-induced emission (AIE) characteristics, new opportunities have opened for in vitro and in vivo imaging. In combination with advanced polymer encapsulation technologies, AIE compounds are now available as LuminiCell ultra-bright, organic nanoparticles that enable long-term cell tracking and imaging for applications such as cancer research and stem cell biology.
  • Need to judge your product? Use HPTLC to get insights into sample composition-S2
    Need to judge your product? Use HPTLC to get insights into sample composition-S2 Melanie Broszat, PhD CAMAG, Muttenz, Switzerland & Michaela Oberle Merck KGaA, Darmstadt, Germany Recorded: Feb 20 2018 56 mins
    Thin-Layer Chromatography has been a well-known method for analysis of botanicals and other complex samples since the end of the 1930s. Today’s modern thin-layer chromatography combines the advantage of analytical robustness and high sample throughput with the possibility to use all kind of specific detection methods, e.g. classical UV/Vis/Fluorescence detection, mass spectrometry or effect-directed analysis. This method increases the amount of information for a fast and efficient screening for new compounds and the identification of raw materials especially for samples with a high matrix load such as herbal drugs, cosmetic and food samples.
    HPTLC, the most advanced form of Thin-Layer Chromatography, is a powerful yet simple and cost effective tool for testing identity, purity, and strength (content) of botanicals as well as excluding adulteration during quality control. With the publication of general chapters by the United States Pharmacopoeia (USP ) and European Pharmacopoeia (Ph.Eur. 2.8.25) HPTLC has officially come into existence as a highly standardized and therefore reproducible analytical technique. The use of high performance plates, suitable instrument and software, a standardized methodology, and validated methods ensures reliable results that are fully compliant with current Good Manufacturing Practice (cGMP). HPTLC fingerprints allow convenient visual comparison of multiple samples even if those originate from different plates (and different laboratories worldwide). Reference images (HPTLC fingerprints of botanical reference materials or other references) can be used to qualify data and pass/fail samples based on similarity or difference.
    We will give you an overview about the versatility of HPTLC, applicable to many of your analytical tasks. Don’t miss the chance to extend your knowledge!
  • Need to judge your product? Use HPTLC to get insights into sample composition
    Need to judge your product? Use HPTLC to get insights into sample composition Melanie Broszat, PhD CAMAG, Muttenz, Switzerland & Michaela Oberle Merck KGaA, Darmstadt, Germany Recorded: Feb 20 2018 52 mins
    Thin-Layer Chromatography has been a well-known method for analysis of botanicals and other complex samples since the end of the 1930s. Today’s modern thin-layer chromatography combines the advantage of analytical robustness and high sample throughput with the possibility to use all kind of specific detection methods, e.g. classical UV/Vis/Fluorescence detection, mass spectrometry or effect-directed analysis. This method increases the amount of information for a fast and efficient screening for new compounds and the identification of raw materials especially for samples with a high matrix load such as herbal drugs, cosmetic and food samples.
    HPTLC, the most advanced form of Thin-Layer Chromatography, is a powerful yet simple and cost effective tool for testing identity, purity, and strength (content) of botanicals as well as excluding adulteration during quality control. With the publication of general chapters by the United States Pharmacopoeia (USP ) and European Pharmacopoeia (Ph.Eur. 2.8.25) HPTLC has officially come into existence as a highly standardized and therefore reproducible analytical technique. The use of high performance plates, suitable instrument and software, a standardized methodology, and validated methods ensures reliable results that are fully compliant with current Good Manufacturing Practice (cGMP). HPTLC fingerprints allow convenient visual comparison of multiple samples even if those originate from different plates (and different laboratories worldwide). Reference images (HPTLC fingerprints of botanical reference materials or other references) can be used to qualify data and pass/fail samples based on similarity or difference.
    We will give you an overview about the versatility of HPTLC, applicable to many of your analytical tasks. Don’t miss the chance to extend your knowledge!
  • Need to validate your genome editing experiment? LentiORF to the rescue!
    Need to validate your genome editing experiment? LentiORF to the rescue! Stacey Ward, PhD Recorded: Jan 24 2018 47 mins
    Whether you are looking to perform gene overexpression studies or validate gene knockdown/knockout results from your RNAi/CRISPR experiments, LentiORFs are your ideal shortcut to protein expression and gene analysis. These genes are available in either pooled or arrayed libraries, and can also be combined into gene family sets and custom clone panels to meet diverse research needs. They are excellent reagents for gain-of-function screens and are a powerful complement to CRISPR and RNAi. In this webinar, we will introduce the LentiORF library and its various formats. We will discuss the application of this technology as it pertains to experimental design, delivery mechanisms, data analysis and target validation. Further, we will present recent data demonstrating clone representation of the library in each of its available formats, along with functional validation of the viral particles at the level of both viral integration and functional RNA expression.

    Our ready-to-use Mission TRC3 LentiORFs allow for stable integration, enrichment of cells, and long-term gene expression in difficult-to-transfect cell lines utilizing our best-in-class lentiviral manufacturing. This collection provides researchers with unique tools to gain insights into gene function by modulating gene and protein expression.
  • Total Nitrogen Test Kits as an Alternative to TKN, Nitrate, and Nitrite Tests
    Total Nitrogen Test Kits as an Alternative to TKN, Nitrate, and Nitrite Tests Taylor Reynolds; Ed Askew Ph.D. Recorded: Dec 14 2017 48 mins
    Currently the US EPA has not promulgated a method for the analysis of Total Nitrogen (TN). Most states that have TN regulations require permitee's to measure TN as the composite of TKN, Nitrate and Nitrite. This approach is costly, time consuming, and also susceptible to variability based on the methods used for the individual analytes Merck, KGaA, Darmstadt, Germany is excited to announce the introduction of a Total Nitrogen Test Method consisting of a single spectrophotometric test kit. The method has been evaluated in a multilab study and this webinar will present the results of that study as well as an overview of the savings made possible by utilizing this new method for testing Total Nitrogen.
  • Rapid Fontana-Masson Staining
    Rapid Fontana-Masson Staining Vikas Palhan, PhD, Senior R&D Scientist, Emerging Biology - Genomics Recorded: Dec 14 2017 23 mins
    In the continued effort to save you time and simplify your workflow, we’ve developed a Fontana-Masson Staining Kit with a protocol that is 30x faster than the standard protocol. In this webinar, the researcher who developed the kit explains how easy it is to use this new rapid microwave technique. If you need to stain melanin and argentaffin cells in tissue sections, take a few minutes to watch this webinar - and save yourself many more minutes in the future by using this kit.
  • USP : Methods for Moisture Determination and Karl Fisher Titration
    USP : Methods for Moisture Determination and Karl Fisher Titration Bruce Herzig, Sr. Application Scientist, Merck KGaA, Darmstadt, Germany Recorded: Dec 7 2017 32 mins
    Many Pharmacopeia materials contain water as hydrates or in adsorbed form. Therefore the determination of the water content is important to maintaining compliance with the Pharmacopeia guidelines. Generally one of the three methods are given in the monographs, Method I (Titrimetric), Method II (Azeotropic), or Method III (Gravimetric). This poster will discuss the application of these methods to moisture determinations focusing on Karl Fischer techniques.
  • SPME Applications in Food Analysis by means of a New Matrix Compatible Coating
    SPME Applications in Food Analysis by means of a New Matrix Compatible Coating Emanuela Gionfriddo, Ph.D.; Research Associate under Prof. Janusz Pawliszyn, University of Waterloo (ON, Canada) Recorded: Dec 7 2017 65 mins
    The complexity and diversity of food matrices, and the need for clean extracts to be injected into analytical instrumentation may imply the use of tedious and time-consuming sample preparation strategies that often produce significant laboratory waste. The development of a new matrix-compatible solid phase microextraction (SPME) coating, namely PDMS/DVB/PDMS, bearing enhanced antifouling properties, enables the analysis of complex food matrices by direct immersion SPME, and helps to overcome issues related to extensive sample pre-treatment and instrumental contamination. This webinar will focus on recent advances made toward the analysis of contaminants in complex food samples by using the new matrix-compatible SPME coating, and will describe the optimization of important parameters to be considered when performing Direct Immersion SPME (DI-SPME).
  • Characterization of sub-visible particles with Amnis imaging flow cytometry
    Characterization of sub-visible particles with Amnis imaging flow cytometry Christine Probst, Application Scientist - Merck KGaA, Darmstadt, Germany Recorded: Nov 30 2017 43 mins
    Multi-spectral imaging flow cytometry (MIFC) is an established analytical method for cellular analysis, however has only recently been evaluated for characterization of sub-visible particles in therapeutic formulations despite numerous favorable attributes including:

    • Simultaneous collection of bright-field, side-scatter, and fluorescent imagery
    • Sensitive detection of particles 100 nm-100 μm
    • High image quality using 20X-60X magnification objectives
    • 100% sampling efficiency using hydrodynamic focusing
    • Small sample volume requirement (20 μL)
    • Linear concentration range up to 100 million/mL
    • Wide flow cell (250 μm) minimizes clogs

    Assorted case studies using MIFC for analysis of protein and vaccine formulations will be presented, with an emphasis on measurements and samples that pose challenges for current techniques- including detection of small and transparent particles, direct analysis of highly concentrated formulations, and fluorescence characterization of particle type, chemical composition, and heterogeneous interactions.
  • CRISPR Activator Libraries: Benefits of a whole genome gain-of-function screen
    CRISPR Activator Libraries: Benefits of a whole genome gain-of-function screen Andrew Ravanelli, Ph.D. Senior R&D Scientist, Genome and Epigenome Editing Recorded: Nov 29 2017 42 mins
    CRISPR-Cas9 nucleases have revolutionized genome editing enabling unprecedented efficiency of targeted mutagenesis. The ability to perform large-scale, whole genome loss-of-function screens has allowed for the rapid identification of gene pathways and targets relevant to drug resistance and disease. The CRISPR SAM activator libraries extend the reach of whole genome screening beyond simple knockout, delivering transcriptional co-regulators to a specific human or mouse target sequence using modified dCas9/gRNA complexes. In this webinar, we will introduce new strategies for forward genetic screening using CRISPR SAM activator libraries. We will discuss the application of this technology as it pertains to experimental design, delivery mechanisms, data analysis, and target validation.

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