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Sigma-Aldrich Scientific Seminars

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  • Quantitative Image Analysis and Emerging Applications on the ImageStream Quantitative Image Analysis and Emerging Applications on the ImageStream Brian Hall, Senior Scientist at Millipore Sigma Recorded: Sep 21 2016 56 mins
    The ImageStream® and FlowSight® are multispectral imaging flow cytometers that generates high resolution images of cells at a rate of 1000’s of cells per second. This allows for the rapid acquisition of tens of thousands of images per sample. Using the IDEAS® image analysis software, the system calculates features based not only on fluorescence intensity but the morphology of that fluorescence as well. This novel approach is able to seamlessly combine the quantitative power of flow cytometry with the high content information associated with microscopy. The system can collect data on a wide range of applications including nuclear localization during a signal transduction cascade, measuring colocalization of two probes, or quantify features on the phagocytosed particles in macrophages.
  • Skin Cancer and Immunohistochemistry Skin Cancer and Immunohistochemistry Jeff Gordon, Director OEM Sales - Cell Marque Corporation, Rocklin CA Recorded: Jun 22 2016 63 mins
    Skin cancer is by far the most prevalent cancer. Each year, approximately 3.4 million people in the US alone are diagnosed with some form of skin cancer. Skin cancer can be highly treatable if it is detected and classified early, and this detection and classification is often aided by immunohistochemistry. This presentation covers many of the basic science, facts, and statistics of skin cancer, as well as the utility of immunohistochemical testing with markers such as S-100, SOX-10, Ber-Ep4, and HHV-8 in the accurate diagnosis and survival rates of skin cancer. Continuing education credits for attending this webinar will be offered through the National Society of Histotechnology
  • Growing Cell Line Potential: From Lab To Licence S2 Growing Cell Line Potential: From Lab To Licence S2 Dr. Lily Chan, Cambridge Enterprise Recorded: Jun 9 2016 26 mins
    A scientific overview of the portfolio of cell lines University of Cambridge has deposited at ECACC with a focus on the KARPAS 299 and KARPAS 422 cell lines; the HeLa Mitotrap cell lines; and CHO cell lines. We will also provide an overview of the process of partnering with ECACC and Sigma-Aldrich for the storage and distribution of cell lines for research purposes. Topics include: The scientific applications of the cell lines; The types of companies and institutions we license to; Advantages of partnering with culture experts and specialised distributors; Our experience of working with ECACC and Sigma-Aldrich.
  • Growing Cell Line Potential: From Lab To Licence S1 Growing Cell Line Potential: From Lab To Licence S1 Dr. Lily Chan, Cambridge Enterprise Recorded: Jun 9 2016 24 mins
    A scientific overview of the portfolio of cell lines University of Cambridge has deposited at ECACC with a focus on the KARPAS 299 and KARPAS 422 cell lines; the HeLa Mitotrap cell lines; and CHO cell lines. We will also provide an overview of the process of partnering with ECACC and Sigma-Aldrich for the storage and distribution of cell lines for research purposes. Topics include: The scientific applications of the cell lines; The types of companies and institutions we license to; Advantages of partnering with culture experts and specialised distributors; Our experience of working with ECACC and Sigma-Aldrich.
  • Sample Preparation for Food Analysis- Part 3 Sample Preparation for Food Analysis- Part 3 Matthias Nold, Product Manager- Merck Recorded: May 27 2016 42 mins
    Part 3 of a 3-part presentation series about food analysis focused on sample preparation and including information about Reference Material
  • Sample Preparation for Food Analysis- Part 2 Sample Preparation for Food Analysis- Part 2 Uwe Wagner, Marketing Manager - Merck Recorded: May 25 2016 33 mins
    Part 2 of a 3-part presentation series about food analysis focused on sample preparation and including information about Reference Material
  • Colorectal Cancer and Immunohistochemistry Colorectal Cancer and Immunohistochemistry Jeff Gordon, Cell Marque- Rocklin, CA Recorded: Mar 30 2016 58 mins
    Colorectal cancer is the third most commonly diagnosed cancer in men and women, and is the second leading over all cause of cancer deaths in the United States. Each year, the CDC reports that approximately 135,000 people are diagnosed with colorectal cancer, and over 50,000 succumb each year to the illness. The National Cancer Institute cites a decline in the mortality rate of colorectal cancer due to advanced screening and early diagnosis. Immunohistochemistry has served as the catalyst for these advancements in colorectal cancer diagnosis. This presentation covers many of the basic science, facts, and statistics of colorectal cancer, as well as the utility of immunohistochemical testing with markers such as cadherin¬17, CDX¬2, SATB2, and the mismatch repair proteins in the accurate diagnosis and survival rates of colorectal cancer.
  • Designing the Next Phase in Genome Editing: Advanced CRISPR Applications Designing the Next Phase in Genome Editing: Advanced CRISPR Applications Jeremy Lehmann- CRISPR Product Specialist, Functional Genomics, MilliporeSigma Recorded: Mar 1 2016 44 mins
    CRISPR Cas9 nucleases have revolutionized the field of genome editing enabling unprecedented efficiency of gene targeting in a vast array of cell types and organisms. Even with such powerful technology at hand, researchers who are new to the field may find genome modification to be challenging and time-consuming. As CRISPR becomes a focus of the molecular biology research community, MilliporeSigma seeks to share the best approaches learned and methods applied in our years of genome editing experience. Today’s presentation will focus on practical applications of CRISPR for pristine genome editing to achieve knockout as well as specific sequence changes to include donor-mediated snps, reporter-tags and conditional knockouts. Special attention will be paid to design considerations for the donor constructs necessary to achieve specific sequence changes. Finally, the frontiers of CRISPR technology, including synthetic crRNA to fast-track genome editing experiments, whole genome screening and targeted gene activation will be explored.
  • The Analysis Workflow of the New EPA Method 325 for Fenceline Monitoring The Analysis Workflow of the New EPA Method 325 for Fenceline Monitoring Jamie Brown - Supelco R&D Scientist , Lee Marotta - PerkinElmer Senior Field Application Scientist Recorded: Feb 17 2016 52 mins
    The new EPA METHOD 325 for sampling Volatile Organic Compounds from Fugitive and Area Sources was promulgated in September of 2015. The petroleum refineries have 2 years to comply with the new ruling. Benzene emissions are the focus of the new ruling, but other VOC’s can be monitored using the same sampling tube. The air samples are collected using thermal desorption tubes that are deployed along fenceline of the property and remain deployed for 14 days. The samples are collected passively, without the using of an air sampling pump. After sampling the diffusive endcap is replaced with a metal storage cap and sent to a laboratory where they’re analyzed by thermal desorption gas chromatography. This is the first of a two series presentation that focuses on the analysis side of the workflow. It provides users the steps required to comply with the new EPA fence line regulations and what is needed to optimize the method for high sample throughput. Refineries and testing laboratories will benefit by gaining valuable information on the optimum techniques to perform this method and what it takes to comply. An introduction to the theory and operation of thermal desorption will also be included.

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