The Corning Scientific Seminar Series is a series of free, online technical presentations that provide novel tips, best practices and proven techniques to help advance your research. Delivered by scientists to scientists, these one-hour broadcasts offer useful information and tips for lab technicians and researchers.
In recent years, three-dimensional (3D) cell culture models have been emerging as the preferred in vitro cancer model as they better mimic the in vivo tumor microenvironment. For instance, 3D cultures develop hypoxic cores and demonstrate gradients of soluble factors and a diffusion profile for drugs similar to solid tumors. Understanding the interactions between cancer cells and other cell types in the tumor microenvironment is also critical to predicting therapeutic efficacy.
In this webinar, Audrey Bergeron will review:
•An introduction to 3D cell culture
•Assay techniques in spheroid microplates
•Several 3D cancer models and technology tools used to better capture the complexity of the tumor microenvironment in assay-compatible formats.
About the Presenter:
Audrey Bergeron is an applications scientist for Corning Life Sciences. She evaluates new products and develops protocols and technical documents using Corning cell culture products. She also provides product training to Corning employees and customers, and assists technical services with customer support by helping to troubleshoot customer experiments.
Franziska Wienholz, Scientific Support Specialist, Corning Life SciencesRecorded: Jan 28 201944 mins
Cell Culture Masterclass: A 10-Point Plan to Prevent Contamination
All cell culture laboratories have experienced it: cell culture contamination and its inevitable loss of material. Contaminants may be biological or chemical, visible or invisible, destructive or seemingly benign, but in all cases, they adversely affect both the use of your cell cultures and the quality of your research.
Identification and, even better, prevention of contamination are crucial for ensuring high-quality research. This expert webinar will present an overview on how to prevent, recognize and tackle contamination by introducing good lab practices and targeted interventions.
If you work with cell culture daily or are interested in setting up prevention strategies to minimize contamination, then join SelectScience and Franziska Wienholz, scientific support specialist of Corning Life Sciences, for this educational masterclass. Franziska will share a 10-point plan to prevent contamination and provide a comprehensive refresher on all aspects of cell culture.
Key learning objectives:
Understand the basic rules of cell culture
Know how to properly manage cell lines to prevent cross-contamination
Be able to implement a 10-point plan to contamination prevention in your laboratory
Who should attend:
Scientists working with cell culture on a daily basis
Cell culture managers who are interested in setting up programs to prevent contamination and determine authentication of cells
Researchers that struggle with contamination issues
Dr. Richard E. Eglen, Vice President and General Manager, Corning Life Sciences.Recorded: Jan 28 201962 mins
Advances in three-dimensional Cell Culture in Drug Research, Discovery and Biologic Manufacture.
Cellular research is optimal when using physiologically relevant cell phenotypes and genotypes of human origin. This assertion has accelerated the adoption of primary cells, stem cells, and, increasingly, patient-specific cells in drug discovery. Over the past five years, technological improvements in 3D cell culture technology, to better mimic in vivo physiology, have also advanced. This is true not only in the areas of cancer and neurological research, but also in the assessment of clinical candidates for metabolic and toxicological liabilities. Furthermore, 3D cell culture is facilitating novel approaches to both the scale-up and manufacture of biologics, including those used in immuno- and stem cell-based therapies.
Are you a scientist interested in 3D cell culture? Join this expert webinar, in which Dr. Richard Eglen will discuss the importance of 3D cell culture in drug discovery and manufacture and how technological improvements are causing significant advancements in this field.
- The existing and future impacts of 3D cell culture technology on fundamental research
- The impacts of 3D cell culture on drug discovery and manufacture, particularly in the context of using phenotypically relevant cells?
- The potential for spheroids, organoids, scaffolds, and hydrogels in cellular research and compound identification, screening, and development?
Harvesting your cells is an important step in maintaining healthy cultures. Good technique will lead to a healthy single cell suspension. There are many harvesting options depending on the cell line and application. In this video, we will discuss some tips/tricks to harvesting cells.
Looking for tips using Matrigel matrix before you coat and plate your cells? We’ve got you covered. Watch our short tutorial for best practices on how to coat Matrigel Matrix as a thin gel layer.
In search of more Matrigel matrix user information? We’ve summarized some other great tips and tricks for Matrigel in our newly published "Ultimate Guide to Matrigel Matrix" – download it today: https://goo.gl/9Nq5Td
Trying to use Matrigel matrix for cell attachment? Watch our short video to learn the best way to coat Matrigel matrix in a thin, non-gelled protein layer.
Interested in some more Matrigel matrix best practices? We’ve summarized some other great tips and tricks for Matrigel in our newly published "Ultimate Guide to Matrigel Matrix" – download it today: https://goo.gl/9Nq5Td
Did you know that cells can be cultured inside Matrigel matrix? Learn more about the “thick-coating” method to embed cells in a 3D, more in vivo-like environment.
Looking for more great Matrigel Matrix tips and tricks? We’ve summarized a bunch in our newly published "Ultimate Guide to Matrigel Matrix" – download it today: https://goo.gl/9Nq5Td
We get a lot of questions on the best ways to thaw Matrigel matrix. Are the color changes normal? What are the best ways to ensure homogeneity? Get the answers to these questions and other best practices for effectively thawing the most widely used ECM in research, Corning Matrigel Matrix. We’ve summarized some other great tips and tricks for Matrigel in our newly published "Ultimate Guide to Matrigel Matrix" – download it today: https://goo.gl/9Nq5Td