Browse communities
Browse communities
Presenting a webinar?

Medical Research

Community information
The medical research community on BrightTALK brings together medical and research professionals. Find relevant webinars on medical research, laboratory science, continuing medical education and more presented by recognized medical researchers. Join the conversation by participating in live webinars and round table discussions on the latest in medical devices, medical research practices and trends in the healthcare industry.
  • Join us for a free webinar on Wednesday 22 April to hear author Professor Malcolm Sperrin talk about his latest book Scientific Basis of the Royal College of Radiologists Fellowship - Illustrated questions and answers.

    During this free live webinar the author will show sample questions and detailed solutions, there will also be a live Q&A session at the end.

    This webinar is recommended listening for anyone training for radiological board exams, graduate students in medical physics, clinicians and technologists. It is also suitable for other board exams or professional development.
  • The webinar will give an overview about general drug delivery requirements for paediatric drug products and current discussions concerning the EU and US regulations.
    In-depth Case Studies featuring Patient-centric Paediatric formulations, will also be presented.
  • Join us on April 16, 2015 @ 7:00PM (EST) for Creating a Positive Patient-Provider Relationship, led by ICA Board member, Joy Selak, PhD, and her healthcare provider, Steven S. Overman, MD, MPH. Drs. Selak and Overman together wrote a book, You Don’t LOOK Sick!: Living Well with Invisible Chronic Illness, about their shared experience. This webinar will focus on how to find effective, supportive care and creating a positive patient-provider relationship.
  • Your environmental testing laboratory is facing harsher EPA emission regulations and competitive sample prices, making is essential to keep up on new technology. The BUCHI Syncore® Parallel Concentrator not only recovers > 95% of solvent but it also provides a crucial competitive advantage. Join this webinar to learn how today’s top environmental testing laboratories are staying ahead of their competition with the implementation of the Syncore® Analyst.
  • Regardless of the colour of the next UK government that emerges as a result of the May election, the £30-£50bn NHS budget chasm is the abyss that all the parties fear in respect of its thereat to quality health service delivery and re-election.

    With political backs to the abyss, no hostages will be taken. It is not unrealistic to expect a well co-ordinated and concerted offence by politicians and patient organisations alike on industry pricing of innovative speciality and rare diseases medicines:

    With this scenario in mind pharmaphorum is once again working with IMS Health to bring you expert insight into what this means for the UK health system and more specifically market access for specialist treatments in the post-election environment.

    Angela McFarlane, Senior principal IMS Health Local Market Access, Anthony Grosso, Principal Pharmacist at the National Hospital for Neurology and Neurosurgery and Sheela Upadhyaya, previously a NHS Senior Manager with over 17 years of experience in the delivery of services and in commissioning of highly specialised services for rare and ultra rare conditions, combine with pharmaphorum's Paul Tunnah to deliver an accurate analysis and frontline insight as to the already complex roadmap for access is about to change again.

    This one hour event will include analysis of informed predictions which include:

    The NICE HTA criteria will get tougher post end to end review (IMMTreV)
    NICE and the NHS England will join forces to streamline access pre/post NICE
    Risk at local level will be shifted to Providers under the new marginal tariff arrangements; which could indeed see a close to the CDF as the PbR excluded drugs list becomes a thing of a glorious past
    Provider baselines will take the strain of innovation - the tough decisions will be softened by a drive to really accelerate uptake of NICE approved medicines

    How can industry prepare for these changes in what is already a tough and complex environment?
  • Although there are many techniques to study epigenetic marks such as DNA- and histone-methylation, on a genomic scale, there exists a need in the field to visualize these epigenetic marks at a single genomic locus in individual cells. Such an application requires a highly sensitive detection method. With this aim, a protocol was developed to perform in-situ hybridization followed by proximity ligation assay (a.k.a. Duolink®) and cell imaging to visualize DNA-methylation (5meC) on the SEPTIN9 promoter. SEPTIN9 promoter methylation is a known biomarker for colon cancer. After optimizing cross-linking, cell permeabilization and chromatin accessibility, the genomic specificity was ascertained by hybridizing with a pool of biotinylated-oligo probes that target the CpG islands in the human SEPTIN9 promoter. The Duolink assay was performed using anti-biotin and anti-5meC antibodies, corresponding proximity ligation assay probes, and Far Red detection reagents.
    Imaging by fluorescent microscopy revealed two red punctate spots in metastatic prostate cancer DU145 cells (diploid for chromosome 17 – location of SEPTIN9 gene) and three red spots in colon cancer SW480 cells (triploid for Chr17). No signal was observed in normal cells (BJ) or with non-specific oligo probes (LacZ). A decrease in Duolink signal was observed when the DU145 cells were treated with 5-AzaC, a drug known to block DNA-methylation. This proof of concept study will be extended to frozen and formalin fixed paraffin embedded human cancer tissue samples.
    Fluorescent imaging data will also be presented from a Duolink assay to monitor the interaction of EZH2 histone methyltransferase with the H3K27me3 epigenetic mark in prostate cancer (DU145) cells. Reduction in the Duolink signal demonstrated inhibition of EZH2 activity by the small molecule inhibitors SAHA and GSK343.
  • Although there are many techniques to study epigenetic marks such as DNA- and histone-methylation, on a genomic scale, there exists a need in the field to visualize these epigenetic marks at a single genomic locus in individual cells. Such an application requires a highly sensitive detection method. With this aim, a protocol was developed to perform in-situ hybridization followed by proximity ligation assay (a.k.a. Duolink®) and cell imaging to visualize DNA-methylation (5meC) on the SEPTIN9 promoter. SEPTIN9 promoter methylation is a known biomarker for colon cancer. After optimizing cross-linking, cell permeabilization and chromatin accessibility, the genomic specificity was ascertained by hybridizing with a pool of biotinylated-oligo probes that target the CpG islands in the human SEPTIN9 promoter. The Duolink assay was performed using anti-biotin and anti-5meC antibodies, corresponding proximity ligation assay probes, and Far Red detection reagents.
    Imaging by fluorescent microscopy revealed two red punctate spots in metastatic prostate cancer DU145 cells (diploid for chromosome 17 – location of SEPTIN9 gene) and three red spots in colon cancer SW480 cells (triploid for Chr17). No signal was observed in normal cells (BJ) or with non-specific oligo probes (LacZ). A decrease in Duolink signal was observed when the DU145 cells were treated with 5-AzaC, a drug known to block DNA-methylation. This proof of concept study will be extended to frozen and formalin fixed paraffin embedded human cancer tissue samples.
    Fluorescent imaging data will also be presented from a Duolink assay to monitor the interaction of EZH2 histone methyltransferase with the H3K27me3 epigenetic mark in prostate cancer (DU145) cells. Reduction in the Duolink signal demonstrated inhibition of EZH2 activity by the small molecule inhibitors SAHA and GSK343.
  • How do ischemia and fixation impact the quality of your assay results and information transfer? Garbage in! Garbage out! Join Geoffrey Baird MD, PhD and David Chafin, PhD at 9 AM Mountain Time, March 31, 2015, who will discuss the impact of ischemia and fixation on assay results, information transfer and some novel approaches to address these important factors. Dr. Baird is an Assistant Professor of Laboratory Medicine and an Adjunct Assistant Professor of Pathology at the University of Washington. Dr. Chafin is a Principle Scientist, Technology and Applied Research at Ventana Medical Systems Inc.
  • C-H functionalization is a powerful addition to the toolbox of the medicinal chemist. Modern C-H functionalization techniques hold the potential to enable the rapid exploration of structure activity relationships (SAR), the generation of oxidized metabolites, the blocking of metabolic hot spots and the preparation of biological probes. This presentation will describe a variety of high-value C-H functionalization chemistries, developed in house and in collaboration with academic groups, and give examples of how these technologies have been deployed successfully to impact drug discovery programs.
  • This webinar will discuss the evolving role of Cell Lines and Cell Culture and their usefulness as models of physiological function and disease. The webinar will review the depth, breadth and best use of currently available Cell Lines. The role played by Cell Lines in ensuring that the output of future research continues to be valid and credible will also be evaluated. This is the first of a three part webinar series focused on delivering a broad picture of the role of cell lines as models in scientific research, the various strategies and approaches available.
  • Abstract:
    There is a great interest in application of human mesenchymal stem cells (hMSCs) in cell therapy and tissue engineering due to their self-renewal, multi-lineage differentiation, immunomodulation, and trophic potential. One of the challenges faced in the clinical application of hMSCs is the need for efficient expansion of these cells in vitro without altering their capacity. Serum-free mammalian cell culture media, in particular, require optimization of the expansion protocols. Even subtle changes in routine handling can have a significant impact on the cells’ potential.

    This seminar will cover the variables that can influence the desired regenerative and differentiation properties including medium selection, vessel surface treatment, impact of the cell source, and seeding density. We will also discuss how users can select the correct conditions for optimized growth and functionality.

    Speaker Biography:
    Brian Posey is a Product Development Manager for cell culture media at Corning Life Sciences. Brian has over 10 years experience in cell biology and industrial scale cGMP manufacturing of both liquid and powder cell culture media. Since joining Corning in 2012, Brian has lead numerous innovative technology projects for the media business ranging from customer technology transfer for production scale-up to developing new serum-free media for industrial and stem cell lines.
  • Mass spectrometry and other methods such as yeast two-hybrid can now accurately discern protein complexes or larger protein interactomes in diseases such as cancer, yet it is difficult to forward translate this knowledge into human samples. To overcome this hurdle, we began experiments using proximity ligation assays (PLA) to directly translate protein complexes into human tumor materials. Our assay reflects protein complexes between EGFR and GRB2 protein, a key adaptor protein necessary for EGFR pathway activation and coupling to downstream MAPK signaling. We annotated nearly 300 primary xenograft models (PDX) of cancer and show tumor subtype enrichment of EGFR:GRB2 signaling-associated complexes. Furthermore, tumors with abundant levels of EGFR:GRB2 signaling-associated complexes are more likely to respond to anti-EGFR antibody-based therapy. Finally, in 350 lung cancer tissues, across three distinct cohorts of patients, we demonstrate the ability of EGFR:GRB2 protein complexes to segregate tumors and show benefit to EGFR tyrosine kinase inhibitor therapy in patients whose tumors harbor high levels of EGFR:GRB2 signaling-associated complexes. This suggests that annotation of signaling-associated protein complexes in cancer tissues can not only molecularly annotate disease types but may also have predictive capacity for cancer therapeutics. This work opens up the human protein interactome as a new class of molecular markers for disease in a more practical manner. Proteins, encoded by DNA, do not work in isolation but instead function as part of multi-protein complexes that drive both normal and disease physiology. While we demonstrate the utility of this approach in cancer, receptor tyrosine kinase signaling and tyrosine kinase inhibitor therapeutics, our approach described here could have utility across a wide spectrum of both signaling-associated complexes and different types of disease, and thus would be attractive to a large audience.
  • This webinar will discuss the evolving role of Cell Lines and Cell Culture and their usefulness as models of physiological function and disease. The webinar will review the depth, breadth and best use of currently available Cell Lines. The role played by Cell Lines in ensuring that the output of future research continues to be valid and credible will also be evaluated. This is the first of a three part webinar series focused on delivering a broad picture of the role of cell lines as models in scientific research, the various strategies and approaches available.
  • Mass spectrometry and other methods such as yeast two-hybrid can now accurately discern protein complexes or larger protein interactomes in diseases such as cancer, yet it is difficult to forward translate this knowledge into human samples. To overcome this hurdle, we began experiments using proximity ligation assays (PLA) to directly translate protein complexes into human tumor materials. Our assay reflects protein complexes between EGFR and GRB2 protein, a key adaptor protein necessary for EGFR pathway activation and coupling to downstream MAPK signaling. We annotated nearly 300 primary xenograft models (PDX) of cancer and show tumor subtype enrichment of EGFR:GRB2 signaling-associated complexes. Furthermore, tumors with abundant levels of EGFR:GRB2 signaling-associated complexes are more likely to respond to anti-EGFR antibody-based therapy. Finally, in 350 lung cancer tissues, across three distinct cohorts of patients, we demonstrate the ability of EGFR:GRB2 protein complexes to segregate tumors and show benefit to EGFR tyrosine kinase inhibitor therapy in patients whose tumors harbor high levels of EGFR:GRB2 signaling-associated complexes. This suggests that annotation of signaling-associated protein complexes in cancer tissues can not only molecularly annotate disease types but may also have predictive capacity for cancer therapeutics. This work opens up the human protein interactome as a new class of molecular markers for disease in a more practical manner. Proteins, encoded by DNA, do not work in isolation but instead function as part of multi-protein complexes that drive both normal and disease physiology. While we demonstrate the utility of this approach in cancer, receptor tyrosine kinase signaling and tyrosine kinase inhibitor therapeutics, our approach described here could have utility across a wide spectrum of both signaling-associated complexes and different types of disease, and thus would be attractive to a large audience.
  • Today use of the term “single use technologies” can be considered as being synonymous with the modern biotech industries. Based on the proliferation of companies now specialising in providing solutions to pretty much anyone anywhere, along with the intensive level of marketing and industry discussion, it is clear to see that the sector has progressed significantly over the years. The ability to purchase systems and technologies off the shelf as a consumable, rather than invest in significant capital infrastructure, better facilitates small enterprises to perform small and pilot GMP scale manufacture in-house. Organisations are able to leverage the experience of the manufacturers in the development of these products. The concept of single use technologies has been proven to produce solutions which are compliant and work. However, there still are questions around regulatory guidance for single use technologies.
  • Within the development of rapid screening methods to secure the global supply chain, the FDA’s Division of Pharmaceutical Analysis (DPA) has been building Raman spectral libraries for verification of pharmaceutical materials, in conjunction with handheld instruments in the field. Focusing on finished products, we evaluate the spectral library for method specificity, showing high specificity for drugs containing different active ingredients.
  • Some of the most critical data required to solve analytical problems in the healthcare and life sciences world lack the type of keys that we rely on so heavily in other domains. This session will focus on solutions to the fundamental data connectivity challenges in this area and how to enable impactful analytics on a few high value but disconnected healthcare data sources.
  • Visit this page to download our infographic:
    http://www.vitaminsinmotion.com/fileadmin/data/images/infographics/Eat_right_for_your_sight_Digital_06.pdf

    The global market for eye health ingredients is increasing fast with a projected compound growth rate of 5.9% until 2019 [1]. The innovation is no longer focusing only on age-related macular degeneration. Supplementation can be beneficial to healthy people of all ages to achieve optimized visual performance and comfort.

    DSM, Kemin and the University of Georgia have recently published a new study to show how lutein and zeaxanthin supplementation can help younger, healthy people improve their vision, such as the ability to see objects under glare conditions.

    Join our webinar and hear lead author Professor Billy R. Hammond Jr. and DSM’s Jens Birrer and Wolfgang Schalch present the latest findings and explain what the new science means to the market.

    [1] Frost&Sullivan (2013). “Strategic Analysis of the Global Eye Health Ingredients Market”.
  • The proximity ligation assay (PLA) is a powerful technique for performing in situ analysis of antibody targets in cells or tissues. This antibody-based application is very robust, and will provide unique data on localization and/or interaction of proteins. The webinar will outline the development of PLA, using an approach that is designed to help all researchers—regardless of their level of experience with this application. Topics addressed will include the following: guidelines for getting started, tips on the crucial bioinformatics investigations involved with the planning phase, specific details on the bench-work associated with performing the assay, and troubleshooting suggestions to allow investigators to refine the assay and successfully integrate it into their research program.
  • This session provides a local Trust perspective on how the Industry Costing Template can be practically embedded for financial management of commercial research studies.

    A brief overview of the national tool principles is provided, but the main focus of the session is on practical examples of how these principles have been locally developed and implemented to support investigator and directorate incentivisation to encourage involvement in commercial contract studies.

    Who should view this session?

    "Financial Management of Commercial Contract Studies: A Trust perspective" is a must see for all NHS staff supporting commercial research including R&D and Finance teams, as well as Local Clinical Research Network staff supporting Trusts in their area to conduct commercial contract research.
  • Visit this page to download our infographic:
    http://www.vitaminsinmotion.com/fileadmin/data/images/infographics/Eat_right_for_your_sight_Digital_06.pdf

    The global market for eye health ingredients is increasing fast with a projected compound growth rate of 5.9% until 2019 [1]. The innovation is no longer focusing only on age-related macular degeneration. Supplementation can be beneficial to healthy people of all ages to achieve optimized visual performance and comfort.

    DSM, Kemin and the University of Georgia have recently published a new study to show how lutein and zeaxanthin supplementation can help younger, healthy people improve their vision, such as the ability to see objects under glare conditions.

    Join our webinar and hear lead author Professor Billy R. Hammond Jr. and DSM’s Jens Birrer and Wolfgang Schalch present the latest findings and explain what the new science means to the market.

    [1] Frost&Sullivan (2013). “Strategic Analysis of the Global Eye Health Ingredients Market”.
  • The proximity ligation assay (PLA) is a powerful technique for performing in situ analysis of antibody targets in cells or tissues. This antibody-based application is very robust, and will provide unique data on localization and/or interaction of proteins. The webinar will outline the development of PLA, using an approach that is designed to help all researchers—regardless of their level of experience with this application. Topics addressed will include the following: guidelines for getting started, tips on the crucial bioinformatics investigations involved with the planning phase, specific details on the bench-work associated with performing the assay, and troubleshooting suggestions to allow investigators to refine the assay and successfully integrate it into their research program.
  • Gap junctions (GJs) are large aggregates of intercellular channels that facilitate the diffusion of small molecules and ions between two interacting cells. GJ intercellular channels are formed through the interaction of two half-channels, called hemichannels, composed of oligomerized connexin protein subunits. Both GJs and hemichannels have numerous important physiological and pathological roles in tissue functions including propagation of the action potential in the heart, tumor growth and metastasis, the inflammatory response and adaptive immunity, wound healing, and electrical synaptic transmission in the central nervous system. The most widely expressed connexin isoform is Cx43, and its regulation in the abovementioned processes has been a major focus of GJ research. Over the past two decades protein-protein interaction with the cytoplasmic carboxyl terminus of Cx43 has come to the fore as an endogenous mechanism for controlling the GJ life cycle, channel gating, and channel-independent functions. We have used the Duolink proximity ligation assay (PLA) as a technique to study protein interactions with Cx43 in cultured cells. Two unique aspects of the technology – specifically, subcellular localization and the binary nature of the labeling – in combination with standard immunofluorescent confocal imaging techniques have yielded unexpected insights into GJ ultrastructure, action potential conduction, and the mechanistic regulation of Cx43 trafficking and hemichannel accretion to GJ plaques. In this context, the practical application of, appropriate controls for, and interpretation of Duolink PLAs will be explicated.
  • Gap junctions (GJs) are large aggregates of intercellular channels that facilitate the diffusion of small molecules and ions between two interacting cells. GJ intercellular channels are formed through the interaction of two half-channels, called hemichannels, composed of oligomerized connexin protein subunits. Both GJs and hemichannels have numerous important physiological and pathological roles in tissue functions including propagation of the action potential in the heart, tumor growth and metastasis, the inflammatory response and adaptive immunity, wound healing, and electrical synaptic transmission in the central nervous system. The most widely expressed connexin isoform is Cx43, and its regulation in the abovementioned processes has been a major focus of GJ research. Over the past two decades protein-protein interaction with the cytoplasmic carboxyl terminus of Cx43 has come to the fore as an endogenous mechanism for controlling the GJ life cycle, channel gating, and channel-independent functions. We have used the Duolink proximity ligation assay (PLA) as a technique to study protein interactions with Cx43 in cultured cells. Two unique aspects of the technology – specifically, subcellular localization and the binary nature of the labeling – in combination with standard immunofluorescent confocal imaging techniques have yielded unexpected insights into GJ ultrastructure, action potential conduction, and the mechanistic regulation of Cx43 trafficking and hemichannel accretion to GJ plaques. In this context, the practical application of, appropriate controls for, and interpretation of Duolink PLAs will be explicated.
  • This webinar will introduce the history, theory, basic techniques, and potential pit-falls of mammalian cell culture. It is designed for students and new lab technicians, as well as bench scientists interested in updating their techniques or knowledge in the field.

    Topics to be discussed include:
    • History and practical theories of cell culture and its impact on today’s science
    • The requirements needed to set up a cell culture laboratory
    • Challenges when performing mammalian cell culture and how to overcome them

    About our Presenter:
    Dr. Mark Rothenberg graduated from Emory University with his Ph.D. in Cell and Developmental Biology. Over the past 25 years, Mark has held positions in both academia and industry where he has developed an expertise in the areas of assay development and cell culture. He currently holds the position of Manager Scientific Training and Education with Corning Life Sciences.
  • La malnutrición es un problema general a nivel mundial, ya que las personas no consumen el nivel suficiente de nutrientes esenciales. A su vez, la obesidad se ha convertido en una preocupación creciente porque se consumen demasiados alimentos para el nivel de actividad. A este ritmo, la sociedad actual no podrá cumplir con los objetivos de la Asamblea Mundial de la Salud (WHA) 2025 en materia de nutrición y es esencial que los programas de nutrición se desarrollen aún más para mejorar la situación nutricional. La fortificación de los alimentos es una manera efectiva de producir alimentos altamente nutritivos y combatir la malnutrición y así lograr un mejor desarrollo socioeconómico. La industria de alimentos, bebidas y suplemento dietario cumple una función clave en la promoción de la nutrición de la salud pública al desarrollar opciones nutritivas, saludables y asequibles para los consumidores.

    En este seminario web informativo, el experto independiente Alfonso Valenzuela Bonomo junto con Hector Cori de DSM, director de Nutrition Science & Advocacy LATAM, debatirán sobre la necesidad de la colaboración entre sectores (gobierno, organizaciones civiles y empresas) para combatir la malnutrición.
  • In many geographical areas, the cost of solvent disposal can be just as high or higher than the cost of purchasing solvent. Also, a refined technical grade solvent can often achieve the purity necessary for many industrial applications. Solvent reclamation, recycling, purification and possibly reselling can mitigate these costs and improve the bottom line.
    During this webinar we will discuss:
    •Low cost solutions to improve the purity of technical grade solvents
    •Solvent reclamation and reselling
    •Solvent recycling
  • La malnutrición es un problema general a nivel mundial, ya que las personas no consumen el nivel suficiente de nutrientes esenciales. A su vez, la obesidad se ha convertido en una preocupación creciente porque se consumen demasiados alimentos para el nivel de actividad. A este ritmo, la sociedad actual no podrá cumplir con los objetivos de la Asamblea Mundial de la Salud (WHA) 2025 en materia de nutrición y es esencial que los programas de nutrición se desarrollen aún más para mejorar la situación nutricional. La fortificación de los alimentos es una manera efectiva de producir alimentos altamente nutritivos y combatir la malnutrición y así lograr un mejor desarrollo socioeconómico. La industria de alimentos, bebidas y suplemento dietario cumple una función clave en la promoción de la nutrición de la salud pública al desarrollar opciones nutritivas, saludables y asequibles para los consumidores.

    En este seminario web informativo, el experto independiente Alfonso Valenzuela Bonomo junto con Hector Cori de DSM, director de Nutrition Science & Advocacy LATAM, debatirán sobre la necesidad de la colaboración entre sectores (gobierno, organizaciones civiles y empresas) para combatir la malnutrición.
  • Because of worldwide demographic changes a dramatic increase of the geriatric population is expected in the next decades. While pediatric drug delivery principles are becoming integrated into the development of new medicines, drug developers are less prepared to appropriately address the specific needs of this booming patient population. This webinar tries to give an overview about the possible drug delivery strategies addressing old age dependent differences. There will be specifically focused on how patient convenience and compliance can be improved, how disease specific drug delivery strategies can help geriatric patients, and how learnings from pediatric drug development can be used as a platform towards geriatric drug delivery.
  • Complex treatment delivery techniques allowing faster and more accurate treatments are rapidly gaining clinical acceptance. These complex deliveries require a comprehensive approach to quality assurance that takes into account the types of treatments being delivered and the range of devices being used for delivery. The commonly used AAPM TG-142 report provides some guidance, but this shouldn’t be considered a stand-alone QA programme; it is a small piece in a comprehensive QA programme. A comprehensive QA programme requires equipment and devices, software, personnel, training, data recording, historical review tools, standard operating procedures, approval mechanisms, and an understanding of how the tests apply to your treatment system. Successfully implementing TG-142 requires more than simply conducting the tests listed in the tables; understanding the natural variation of processes, preparation and planning is also needed so that all the necessary aspects are considered to allow for safe delivery of complex treatment delivery techniques. Join Dr Moseley as he discusses the planning and decisions that are needed to be made so that you can succeed at implementing your TG-142 programme.
  • The article considers the opportunities for risk based change in facility design through quality by design (QbD) and advances in PAT. It suggests that the inclusion of a mix of biopharmaceutical products alongside oral solid dose products could work based on a manufacturing dancefloor concept. Further that both upstream and downstream may be considered in a new light with the potential for in-process real-time testing and approval significantly reducing or withdrawing entirely the need for work-in-progress (WIP) inventory and quarantine storage needs as supply chain management processes integrate.
  • Multiplexed protein assays have tremendous potential in clinical diagnostics, in particular measurement of proteins in plasma or serum derived from circulating blood. The dynamic range of protein concentration in these samples, and the dominance of very high abundance proteins such as albumin and immunoglobulins, make measurement of low concentration proteins impossible without some type of enrichment approach. This workshop will discuss the benefits of using Seppro® protein depletion columns in sample preparation for multiplexed LC-MS protein clinical assays.
  • Multiplexed protein assays have tremendous potential in clinical diagnostics, in particular measurement of proteins in plasma or serum derived from circulating blood. The dynamic range of protein concentration in these samples, and the dominance of very high abundance proteins such as albumin and immunoglobulins, make measurement of low concentration proteins impossible without some type of enrichment approach. This workshop will discuss the benefits of using Seppro® protein depletion columns in sample preparation for multiplexed LC-MS protein clinical assays.
  • Originally launched in 2001, Enzyme Explorer was Sigma-Aldrich's first Web-based tool developed to provide customers with on-line technical resources to aid in their research and manufacturing applications. Since that time, Sigma scientists and curators have continually added content and new functionality.
    One example of the tools available is the Enzyme Explorer’s Assay Library. It features over 600 detailed procedures for measuring enzyme activity and related metabolites. The Library is the result of over ten years of in-house process development by Sigma-Aldrich scientists. In addition, we have recently upgraded the classical Nicholson Metabolic Pathway Chart. Now all metabolites, enzymes, and selected pathways are searchable and interactive.
    The Enzyme Explorer also provides easy access to the most extensive line of enzyme, proteins, substrate, inhibitor and cofactor related products in the industry.
    This webinar will give an overview of all the tools and content available and help users navigate to the content they need.
  • Originally launched in 2001, Enzyme Explorer was Sigma-Aldrich's first Web-based tool developed to provide customers with on-line technical resources to aid in their research and manufacturing applications. Since that time, Sigma scientists and curators have continually added content and new functionality.
    One example of the tools available is the Enzyme Explorer’s Assay Library. It features over 600 detailed procedures for measuring enzyme activity and related metabolites. The Library is the result of over ten years of in-house process development by Sigma-Aldrich scientists. In addition, we have recently upgraded the classical Nicholson Metabolic Pathway Chart. Now all metabolites, enzymes, and selected pathways are searchable and interactive.
    The Enzyme Explorer also provides easy access to the most extensive line of enzyme, proteins, substrate, inhibitor and cofactor related products in the industry.
    This webinar will give an overview of all the tools and content available and help users navigate to the content they need.
  • While cost is a primary "c" driving the adoption of object-based cloud solutions in the life sciences, compute, capacity, and collaboration may all be bigger incentives. In this webinar, we'll examine how to use an Avere Hybrid Cloud NAS infrastructure to gain big benefits in areas like genomics research, personalized medicine, drug discovery, imaging, and other data analysis applications.

    • Compute - Building production environments in the compute cloud without rewriting existing applications

    • Capacity - Modernizing storage archives and disaster recovery by adding object storage for durability while leveraging existing on-premises NAS

    • Collaboration - Using the cloud t o safely and securely share data globally

    • Cost - Using cloud to lower overall costs to keep pace with fast-growing demands of research initiatives
  • Parents should be given full information on the benefits of human milk for their newborns, as well as the health consequences of not using human milk, and the risks and costs of formula feeding.
  • In our aging society, Alzheimer’s type dementia is a major concern. The disease can have a devastating impact on quality of life, with patients relying on caregivers for standard daily living activities. Whether it is memory impairment, deterioration, confusion or anger suffered by patients, this highly disruptive neurological disease demands attention.

    Vitamin E is vital for neural function and its deficiency can lead to neurological dysfunction, causing problems with co-ordination, balance and speech. It also has an antioxidant function in the brain – as a free radical scavenger and constituent of neural membranes, preventing the oxidation of lipids and PUFAs.

    Now, recent studies have found that maintaining a high level of vitamin E results in marked improvements for mental health and can be linked individually with a decrease in the onset of Alzheimer’s type dementia.

    Join our webinar and hear our speakers Prof. Maurice Dyksen and DSM’s Dr. Manfred Eggersdorfer and Jacob Bauly present their latest findings on vitamin E and its potential role in curbing the onset of Alzheimer’s disease.
  • In the present study, a genome-wide RNA interference screen was combined with an extensive biochemical analysis and quantitative proteomics to better understand the regulation of the heat-shock response (HSR) upon thermal stress. The usage of an endoribonuclease-prepared small interfering RNA (esiRNA) library represented a simple and efficient way to perform RNAi with minimal off-target effects. In the screening experiments novel positive and negative modulators of the stress response were identified, including proteins involved in chromatin remodeling, transcription, mRNA splicing, DNA damage repair, and proteolytic degradation. The diversity of the identified regulators suggests that induction and attenuation of the HSR integrate signals from different cellular pathways and are rather multi-factorial processes than single gene/protein events. The modulator proteins are localized in multiple cellular compartments with the majority having their primary location in the nucleus. A protein-protein interaction analysis revealed a HSR regulatory network, with chromatin modifiers and nuclear protein quality control components occupying hub positions. These observations are supported by quantitative proteomics experiments, which showed specific stress-induced reorganizations of the nuclear proteome, including the transient accumulation of chaperones and proteasomal subunits.
    Moreover, we found that the acetyltransferase EP300 controls the cellular level of activatable HSF1. This involves acetylation of HSF1 at multiple lysines not required for function and results in stabilization of HSF1 against proteasomal turnover. Acetylation of functionally critical lysines during stress serves to fine-tune HSF1 activation. Finally, the nuclear proteasome system functions in attenuating the stress response by degrading activated HSF1 in a manner linked with the clearance of misfolded proteins.
  • In our aging society, Alzheimer’s type dementia is a major concern. The disease can have a devastating impact on quality of life, with patients relying on caregivers for standard daily living activities. Whether it is memory impairment, deterioration, confusion or anger suffered by patients, this highly disruptive neurological disease demands attention.

    Vitamin E is vital for neural function and its deficiency can lead to neurological dysfunction, causing problems with co-ordination, balance and speech. It also has an antioxidant function in the brain – as a free radical scavenger and constituent of neural membranes, preventing the oxidation of lipids and PUFAs.

    Now, recent studies have found that maintaining a high level of vitamin E results in marked improvements for mental health and can be linked individually with a decrease in the onset of Alzheimer’s type dementia.

    Join our webinar and hear our speakers Prof. Maurice Dyksen and DSM’s Dr. Manfred Eggersdorfer and Jacob Bauly present their latest findings on vitamin E and its potential role in curbing the onset of Alzheimer’s disease.
  • In the present study, a genome-wide RNA interference screen was combined with an extensive biochemical analysis and quantitative proteomics to better understand the regulation of the heat-shock response (HSR) upon thermal stress. The usage of an endoribonuclease-prepared small interfering RNA (esiRNA) library represented a simple and efficient way to perform RNAi with minimal off-target effects. In the screening experiments novel positive and negative modulators of the stress response were identified, including proteins involved in chromatin remodeling, transcription, mRNA splicing, DNA damage repair, and proteolytic degradation. The diversity of the identified regulators suggests that induction and attenuation of the HSR integrate signals from different cellular pathways and are rather multi-factorial processes than single gene/protein events. The modulator proteins are localized in multiple cellular compartments with the majority having their primary location in the nucleus. A protein-protein interaction analysis revealed a HSR regulatory network, with chromatin modifiers and nuclear protein quality control components occupying hub positions. These observations are supported by quantitative proteomics experiments, which showed specific stress-induced reorganizations of the nuclear proteome, including the transient accumulation of chaperones and proteasomal subunits.
    Moreover, we found that the acetyltransferase EP300 controls the cellular level of activatable HSF1. This involves acetylation of HSF1 at multiple lysines not required for function and results in stabilization of HSF1 against proteasomal turnover. Acetylation of functionally critical lysines during stress serves to fine-tune HSF1 activation. Finally, the nuclear proteasome system functions in attenuating the stress response by degrading activated HSF1 in a manner linked with the clearance of misfolded proteins.
  • Microbiological methods play a critical role in ensuring safety and efficacy of drugs and biologics. Most microbiological methods are described in compendia and do not need method validation. However, rapid microbiological methods and other alternate methods require full validation. Due to large inherent variability and complexity of microbiological methods, there are challenges in validation of such methods. This webinar will discuss, ways of performing validation of microbiological methods, with a view to achieving regulatory compliance.
  • Whether you are an experienced researcher or just starting out in your research career, you’ve heard about how important search engine optimization (SEO) is in today’s ocean of online media. You probably know that most researchers have concerns about how “discoverable” their work is amidst the global acceleration of research output in recent years and the increasing sophistication of search engines. Publishing in high-impact journals helps your work get discovered – it’s true - but there are also approaches that you can employ while writing to craft your work for increased online discoverability once it is published. Jamie Wielgus, Associate Editor, Health Sciences, Wiley, and Kris Bishop, Marketing Manager, Wiley, present on some of these techniques and the factors at work behind discoverability with the goal of helping authors take action at the writing stage to best position their articles to be found and read.

    In this interactive 60 minute webinar Jamie and Kris will cover the following topics:

    - The Abstract: What is it for? How should it be structured? Examples?
    - The Title: What makes a good title? Examples?
    - Keywords: How should authors use keywords to be found by readers using today’s search engines?
    - Search Engine Optimization (SEO): Latest advice on SEO? How can research be optimized for search?
    - Discoverability: Pulling it all together!
    - Questions and Feedback
  • The spread of counterfeit and substandard medicines is a global threat to public health. The sources of most counterfeit medicines found globally were traced to Asia. Although many of these products were found outside the region, many Asian countries are at high risk of exposure to them. There is a need for easy and affordable tools for screening medicines. Handheld Raman devices have gained increasing interest as such tools. Many of the devices available share some advantages in the detection of counterfeit medicines but there are challenges in the detection of substandard products. Fixed-dose combination medicines present even greater challenges.