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Corning Scientific Seminar Series

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  • In Vitro Characterization of Species Difference of OATP In Vitro Characterization of Species Difference of OATP Na Li, Ph.D. Recorded: May 25 2016 52 mins
    Organic anion-transporting polypeptides (OATPs) play an important role in hepatic uptake of a variety of clinically important drugs. The significant differences in OATP/Oatp-dependent drug transport between human and preclinical species presents a challenge for interspecies extrapolation of drug pharmacodynamics and pharmacokinetics. The assessment of the difference in hepatic uptake between species using an in vitro model is highly desired to support mechanistic studies and to understand the differences observed between species in vivo.

    The Corning® TransportoCells™ model has demonstrated significant value in terms of supporting in vitro assessment of drug interaction with SLC transporters in drug discovery and development. Recently, several animal species of Oatps were introduced into the Corning TransportoCells portfolio. This webinar will focus on the development of the newly available animal species and characterization of the differences in OATP/Oatp in substrate specificity and kinetics using this "thaw and go" model.
  • Specialized Applications Using the Corning® Spheroid Microplate Specialized Applications Using the Corning® Spheroid Microplate Hilary Sherman, Applications Specialist & Audrey Bergeron, Application Specialist Recorded: Apr 22 2016 47 mins
    Three-dimensional (3D) cell culture models, which offer significant improvements over traditional two dimensional monolayer cell culture in terms of maintaining morphological and functional characteristics of tissue, are increasingly being incorporated in drug discovery as model systems to study disease or for screening for chemotherapeutic efficacy or drug toxicity. This presentation will demonstrate the use of Corning® spheroid microplates to culture and assay spheroids in a rapid and highly reproducible format that enables the formation of a single multi-cellular spheroid, centered in each well.

    Corning spheroid microplates are multiple well, cell culture plates with opaque walls and unique clear, round well-bottom geometry that utilize the Corning Ultra-Low Attachment surface coating. The coating is hydrophilic, biologically inert and non-degradable. Representative data will be shown exhibiting the use of the spheroid microplate in more specialized assays including the formation of neurospheres from neural stem cells (NSCs), a valuable model to study neurogenesis and neural development, as well as analysis of NSC proliferation and migration. We will also demonstrate the formation of hepatospheres using Corning HepatoCells, an immortalized alternative to primary human hepatocytes, in combination with the SCREEN-WELL Hepatotoxicity library from Enzo Life Sciences for 3D hepatotoxicity screening. Finally, we will show the use of the Corning spheroid microplate to generate spheroids comprised of multiple cell types, demonstrating the impact that including multiple cell types in 3D assays can have on therapeutic outcome in a chemotherapeutic assay.
  • There’s More to Choosing a Filter Than You Might Think There’s More to Choosing a Filter Than You Might Think Mark Rothenberg, Ph.D. Recorded: Feb 25 2016 54 mins
    Although filtration is commonly practiced in many laboratories, it is a critical step in preparing media and buffers, clarifying samples, removing microbial contaminants, concentrating and inline filtering gases, to name a few applications. In order to choose the correct filter for your application, you need to consider the application, the material(s) to be filtered, and other factors that will be covered in this presentation. This webinar will focus on:

    An Introduction to Filtration:
    • Basic principles of filtration
    • Overview of membranes
    • Overview of formats

    Choosing the Correct Filtration Product for your Application:
    • Sterilization
    • Clarification of solutions
    • Ultracentrifugation

    About our Presenter:

    Dr. Mark Rothenberg graduated from Emory University with his Ph.D. in Cell and Developmental Biology. Over the past 25 years, Mark has held positions in both academia and industry where he has developed an expertise in the areas of assay development and cell culture. He currently holds the position of Manager Scientific Training and Education with Corning Life Sciences.
  • A New SLC Transporter Model to Study the Impact of OATP1B1 Genetic Variants A New SLC Transporter Model to Study the Impact of OATP1B1 Genetic Variants Na Li, Ph.D. Recorded: Nov 16 2015 48 mins
    The importance of genetic variations in drug transporters for drug disposition and response has been increasingly recognized in the past decade. The drug transporter organic anion transporting polypeptide 1B1 (OATP1B1) is genetically polymorphic and plays a major role in hepatic uptake of a variety of clinically important drugs. Predicting the pharmacokinetic effect of these genetic variants on the drug disposition is critical for understanding the inter-individual variations to drug efficacy and safety. Corning TransportoCells was introduced to support in vitro assessment of drug interaction with SLC transporter. Specially, the newly developed TransportoCells OATP1B1 SNPs, alongside with OATP1B1 wild type, offer an in vitro tool kit for studying the potential impact of genetic variants on drug PK.

    This webinar will focus on the development and characterization of the newly available TransportoCells OATP1B1 genetic variants, three animal species Oatp family members and other drug transporters of emerging importance in drug development.
  • Corning® HepatoCells: A Novel Cell-based Model for In Vitro ADME/Tox Studies Corning® HepatoCells: A Novel Cell-based Model for In Vitro ADME/Tox Studies Rongjun, Zuo, Ph.D. Recorded: Oct 7 2015 49 mins
    Primary human hepatocytes (PHHs) and other hepatic cell models possess several limitations. For instance, PHH’s large lot-to-lot variation requires qualification tests with each lot, resulting in high costs and increased lead time. Furthermore, other non-primary hepatic cells can have insufficient fold induction in some lots and conditions.

    This presentation will introduce Corning® HepatoCells for ADME/Tox studies. Derived from primary human hepatocytes, Corning HepatoCells are a renewable, hepatocyte-like cell line that retains most of the physiological properties of their parental hepatocytes such as mature hepatocyte-like morphology and induction response to prototypical inducers of CYP3A4, 1A2, and 2B6. Characterization of Corning HepatoCells for ADME/Tox studies will be presented, along with data demonstrating how the model system can be used for prediction of clinical CYP induction.
  • Your In Vitro ADME CRO: New Assays for Transport, Enzyme Inhibition & Induction Your In Vitro ADME CRO: New Assays for Transport, Enzyme Inhibition & Induction David M. Stresser, Ph.D. Recorded: Jul 29 2015 60 mins
    Achieving your ADME/Tox testing goals requires experience, quality data, and proper alignment with regulatory guidance. Failure to meet these important requirements can put your drug discovery and pre-clinical goals at risk.
    This presentation will provide an informative overview of how you can advance and reach your pre-clinical drug discovery goals. It will discuss the importance of core contract research capabilities, including enzyme induction, enzyme inhibition, and transporter interaction. In addition, we will review new capabilities and opportunities including CYP induction and SLC transporter assay services – all designed to align with regulatory agency guidance documents.

    Speaker Bio:
    David Stresser is the Program Manager of Corning® Gentest℠ Contract Research Services at Corning Life Sciences since 2001, having held prior positions of Product Manager and Study Director since joining Corning in 1998. Prior to this, he was a post-doctoral associate in the laboratory of David Kupfer at the University of Massachusetts Medical School in Worcester, Massachusetts. He did his graduate work in the laboratory of David E. Williams at Oregon State University in Corvallis, Oregon receiving a Ph.D. in toxicology in 1994. Dr. Stresser has authored or co-authored 40 articles or book chapters in the field of drug metabolism and has been an invited speaker at various national and international meetings, pharmaceutical companies, and universities.
  • Authentication of Cell Lines for Pre-Clinical Cancer Research Authentication of Cell Lines for Pre-Clinical Cancer Research Yvonne A. Reid, Ph.D. Recorded: Jun 22 2015 58 mins
    Join us on June 22nd for a special Corning-sponsored webinar presented by ATCC®.

    Abstract:
    Animal cell lines are important in vitro systems and tools for scientists in diverse disciplines such as basic cell biology, genetic mapping, gene expression and gene therapy. Cell line authentication and characterization are crucial in these activities, yet they are underappreciated by most research scientists. Over the years numerous cell lines have been shown to be misidentified due, in part, to poor techniques and inadequate verification of cell line authenticity. Technological advances have given rise to improved capabilities. Cell line authentication now requires a comprehensive strategy that employs several complementary technologies for systematic testing for morphology, microbial contaminations, cellular identity/cross-contamination as well as functionality. The validity of conclusions drawn from research data is dependent on consistent and unequivocal verification of cell line identity and function. It is estimated that the financial loss incurred by poorly characterized or misidentified cell lines is in the millions of dollars. An overview of the current technologies used to authenticate cell lines will be presented.

    Speaker Bio:
    Dr. Yvonne A. Reid joined ATCC in 1980 and during the mid-1980’s her research focused on the use of DNA hypervariable probes for the intraspecies identification of cell lines. The evolution of this work has led to the implementation of routine screening of all human cell lines by STR analysis. She co-chaired the ATCC SDO committee on the Development Consensus Standard on the Authentication of Human Cell Lines: Standardization of STR profiling. Dr. Reid has more than 30 years of experience in cell biology, immunology and molecular biology. As Collection Scientist for the Cell Biology Program for over 10 years, she was responsible for acquisition of new animal cell lines and hybridomas into the Cell Biology General Collection.
  • An Introduction To Cell Culture An Introduction To Cell Culture Mark Rothenberg, Ph.D. Recorded: Apr 30 2015 58 mins
    This webinar will introduce the history, theory, basic techniques, and potential pit-falls of mammalian cell culture. It is designed for students and new lab technicians, as well as bench scientists interested in updating their techniques or knowledge in the field.

    Topics to be discussed include:
    • History and practical theories of cell culture and its impact on today’s science
    • The requirements needed to set up a cell culture laboratory
    • Challenges when performing mammalian cell culture and how to overcome them

    About our Presenter:
    Dr. Mark Rothenberg graduated from Emory University with his Ph.D. in Cell and Developmental Biology. Over the past 25 years, Mark has held positions in both academia and industry where he has developed an expertise in the areas of assay development and cell culture. He currently holds the position of Manager Scientific Training and Education with Corning Life Sciences.
  • Tips and Techniques for Serum-free Expansion of hMSCs Tips and Techniques for Serum-free Expansion of hMSCs Brian Posey Recorded: Mar 26 2015 53 mins
    Abstract:
    There is a great interest in application of human mesenchymal stem cells (hMSCs) in cell therapy and tissue engineering due to their self-renewal, multi-lineage differentiation, immunomodulation, and trophic potential. One of the challenges faced in the clinical application of hMSCs is the need for efficient expansion of these cells in vitro without altering their capacity. Serum-free mammalian cell culture media, in particular, require optimization of the expansion protocols. Even subtle changes in routine handling can have a significant impact on the cells’ potential.

    This seminar will cover the variables that can influence the desired regenerative and differentiation properties including medium selection, vessel surface treatment, impact of the cell source, and seeding density. We will also discuss how users can select the correct conditions for optimized growth and functionality.

    Speaker Biography:
    Brian Posey is a Product Development Manager for cell culture media at Corning Life Sciences. Brian has over 10 years experience in cell biology and industrial scale cGMP manufacturing of both liquid and powder cell culture media. Since joining Corning in 2012, Brian has lead numerous innovative technology projects for the media business ranging from customer technology transfer for production scale-up to developing new serum-free media for industrial and stem cell lines.
  • A Novel “Thaw and Go” Cell-based Model to Study SLC Transporters A Novel “Thaw and Go” Cell-based Model to Study SLC Transporters Na Li, Ph.D. Recorded: Feb 24 2015 59 mins
    Abstract:
    As a key determinant of drug pharmacokinetics, transporter mediated drug-drug interaction has garnered significant attention from the pharmaceutical industry and regulatory authorities. Corning offers a comprehensive list of tools to support drug transporter studies and recently introduced Corning® TransportoCells™ products to support in vitro assessment of drug interaction with SLC transporters. This new model provides a convenient “thaw and go” cell-based model with robust activity and consistent performance. In this webinar, we will provide an overview of Corning TransportoCells products along with applications for in vitro-to-in vivo correlation. Validation data will also be presented for the newly available TransportoCells products, including OATP1A2, OATP2B1, PEPT1, PEPT2, and NTCP.

    Presenter Biography:
    Dr. Na Li received her B.S degree in Biology from Fudan University, Shanghai, China, and her Ph.D. in Pharmacology from Dartmouth Medical School, Hanover, NH. Her major research focus is on drug transporters, including interspecies differences in hepatobiliary transporters, transporter quantification, and in vitro-to-in vivo extrapolation of drug pharmacokinetics. At Corning, Dr. Li contributes her expertise in in vitro drug transporter technology and its application in drug ADME.

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