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Primer-dimer Formation: The Problem and the Solution

Primer-dimer formation has been always considered as the nemesis not only on sequencing but also on real-time PCR. The presence of primer-dimers in real-time PCR can elevate the background signal altering the Ct value and thus providing misleading expression levels.

In this seminar we
Primer-dimer formation has been always considered as the nemesis not only on sequencing but also on real-time PCR. The presence of primer-dimers in real-time PCR can elevate the background signal altering the Ct value and thus providing misleading expression levels.

In this seminar we will discuss the primer-dimer formation effect on various genomic downstream applications, the approaches to reduce the potential of its formation, and the pros and cons of different techniques of removing primer-dimers post-PCR.
Recorded Jan 10 2012
57 mins
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Presented by
Dr. Bassam El-Fahmawi
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  • Title: Primer-dimer Formation: The Problem and the Solution
  • Live at: Jan 10 2012 5:00 pm
  • Presented by: Dr. Bassam El-Fahmawi
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