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Cell Culture Contamination Part 2

Recent studies have shown that at least 20% of the animal cell cultures currently in use in the US are contaminated by either microorganisms or other cell lines! This three part live, on-line seminar series will review the scope of this major problem and examine some of its causes and techniques for avoiding it.  It will also explore some key, easy to employ strategies for preventing these losses by careful culture management. This webinar will discuss good asceptic technique: developing a practical approach to asceptic technique; reducing day-to-day contamination problems in the lab; helpful hings for avoiding contamination.
Recorded Feb 10 2011 43 mins
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Presented by
John So, M.S.
Presentation preview: Cell Culture Contamination Part 2

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  • Modeling NAFLD and TGFβ-induced Fibrosis in 3D Bioprinted Human Liver Tissue Oct 5 2017 4:00 pm UTC 60 mins
    Jeff Irelan, Ph.D.
    We hope you join us for this special webinar. Jeff Irelan, Director of Scientific Applications for Organovo will be our guest presenter.

    Abstract:
    Nonalcoholic fatty liver disease (NAFLD) is the most common liver disorder with an estimated prevalence of over 25% worldwide and is projected to become the leading indication for liver transplant by 2025. Despite decades of research focused on NAFLD, an effective treatment has yet to be approved. This is due in part to the reliance on cell culture and animal models that present challenges in translation due to limited functional longevity and species differences, respectively.

    ExVive™ 3D Bioprinted Human Liver Tissue, a clinically-translatable in vitro model, is ideal for studying the effects of drugs on liver disease progression, regression, and the mechanisms involved. Here, we present results showing a nutrient overload induction of liver disease and TGFβ-induced fibrosis in ExVive™ Human Liver Tissue. A variety of disease-relevant phenotypes including steatosis, inflammation, and fibrosis can be demonstrated in the model:
    •Nutrient overload leads to the accumulation of lipid droplets in hepatocytes.
    •Incorporation of Kupffer cells and stimulation induces inflammatory cytokine release.
    •Chronic exposure to nutrient overload leads to stellate cell activation and fibrosis.
    •Chronic exposure to chemical inducers of fibrosis or TGFβ stimulation leads to stellate cell activation and fibrosis.
    •A TGFβR1 kinase inhibitor effectively blocks TGFβ-induced fibrosis.

    Presenter Bio:
    Jeff Irelan holds a Ph.D. in molecular biology from the University of Oregon. As Director of Scientific Applications, Jeff interfaces with Organovo’s customers and R&D team to implement and expand the company’s portfolio of service offerings utilizing bioprinted tissue models.
  • New Technologies for Cellular Research: 3-Dimensional Cell Culture and Screening Sep 22 2017 4:00 pm UTC 60 mins
    Richard M. Eglen, Ph.D
    It is now recognized that target and compound identification, as well as validation, are better conducted using cells with physiologically relevant phenotypes and genotypes. This assertion has accelerated the adoption of primary cells, stem cells, or patient-specific cells in cellular research, in general; and drug discovery, in particular.

    Technological improvements in three-dimensional (3D) cell culture technology, as a means to better mimic in vivo physiology, have accelerated recently—not only in the areas of cancer and neurological research, but also for the assessment of compound metabolic and toxicological liabilities. Furthermore, 3D cell culture can provide novel approaches to the scale-up and manufacture of biologically based medicines, including those used in immuno-and stem cell-based therapies.

    In this presentation, the existing and future impact of 3D cell culture technology on fundamental research, and drug discovery and manufacture will be addressed, particularly in the context of using phenotypically relevant cells. Specifically, it will discuss the potential for spheroids, organoids, scaffolds, and hydrogels in cellular research and compound identification, screening, and development.

    Future directions will also be covered, including organs-on-chips, hydrostatic flow technologies, microfluidics, and 3D bioprinting. Some of these approaches will allow for real-time observation of cellular responsiveness to novel compounds and drugs … boldly taking the researcher into a fourth dimension of 3D cell culture!
  • 浮遊系細胞培養用シングルユースシステム Recorded: Sep 12 2017 60 mins
    コーニングインターナショナル株式会社 ライフサイエンス事業部 石渡孝至
    抗体ワクチンやウイルス産生のラボスケールから、治験薬製造スケールまでの浮遊系細胞培養用シングルユース製品の導入ポイントについて概説いたします。

    近年、バイオ医薬品開発に於けるシングルユース製品の市場が活況を呈しております。抗体ワクチン、幹細胞/ヒトiPSや浮遊系細胞による製剤化やバイオバンキン グを具現化する際に、最も大きな懸案事項となりますのが“質”、“量”そして“コスト”の問題があります。そこでコーニングでの安全、安価かつ簡便なシングルユース製品の特徴や仕様についてご紹介致します。
     また治験薬に向けた品質規格や導入事例によるマーケットトレンドを示しながら、トータルソリューションシステムの振盪培養(フラスコ、べセル、バッグ)、チュービング&コネクション(閉鎖系システム、無菌接続)、パッケージング(培養 / 回収 / 凍結保存)および品質規格(施設環境、BSE/TSE、発熱物質(Pyrogen)、USPクラス6規格準拠 / 滅菌保証レベル / エンドトキシン規格 / QC試験)について概説します。
  • バイオ医薬品製造におけるシングルユースシステム Recorded: Jun 6 2017 77 mins
    コーニングインターナショナル株式会社ライフサイエンス事業部 石渡孝至
    バイオロジクスの治験薬製造におけるシングルユース技術導入ポイントであります製品ソリューション、クローズドシステムおよび品質保証体制について概説します。

    再生・細胞医薬を中心にバイオ医薬品の製造開発が急速に進められております。

    幹細胞やヒトES / iPS細胞による再生医療を具現化する際に、最も大きな懸案事項となりますのが“質”、“量”そして“コスト”の問題があります。

    そこでコーニングは、安全、安価かつ簡便な最新シングルユース技術についてレギュレーション、導入事例によるマーケットトレンドを示しながら、トータルソリューションシステムのセルカルチャー(多層式大量培養容器)、チュービング&コネクション(プレアッセンブル特注、無菌接続)、パッケージング(培養 / 回収 / 凍結保存)、データインテグリティ(バーコードによるトレーサビリティ管理)、品質規格(成型施設環境、BSE/TSE、発熱物質(Pyrogen)、USPクラス6規格準拠 / 滅菌保証レベル / エンドトキシン規格 / QC試験)および品質に関するドキュメンテーションサポート(証明書、バリデーションバインダ)に関する情報を概説します。
  • Enabling CAR-T Screening in 3D Tumor Spheroids Recorded: Mar 30 2017 62 mins
    Van Dang, D.V.M., Ph.D., Abhi Saharia, Ph. D., and Audrey Bergeron, Applications Scientist
    Webinar Date & Time: Mar 30 2017 12:00 p.m. EST

    Chimeric antigen receptor (CAR)-T cells, which are engineered to recognize target cell surface antigens expressed on tumor cells, have shown promise to affect complete remission in patients with B-cell malignancies. However, applying this approach to target solid tumors has resulted in adverse effects in clinical studies. Methods for testing different models of CAR-T cells in vitro can provide further insight into viable antigen targets. Historically, two-dimensional (2D) cell culture models have been used in drug discovery. However, more elaborate, three-dimensional (3D) cell culture models better mimic the in vivo tumor microenvironment and help bridge the gap between in vitro studies and clinical outcomes.

    In this special joint webinar, panelists from ProMab Biotechnologies, DiscoverX, and Corning Life Sciences will present data on a high-throughput, easy-to-use, highly reproducible method for screening CAR-T cells in a 3D cell culture model by combining various technologies.


    Speakers:
    Van Dang, D.V.M., Ph.D.
    Scientist and coordinator for CAR-T research
    ProMab Biotechnologies, Inc.

    Abhi Saharia, Ph. D.
    Director, Cell-based Assays and Biologics
    DiscoverX

    Audrey Bergeron
    Applications Scientist
    Corning Life Sciences
  • Surfaces for Organoid Culture Recorded: Feb 23 2017 50 mins
    Nitin Kulkarni, Ph.D.
    3D culture is gaining pivotal importance for attaining in vivo-like conditions in a dish to study developmental cues as well as therapeutic possibilities. Organoid development promises to be one of the most important research tools in the near future. This presentation will cover:

    • Methodologies used in organoid culture
    • Matrices for growing organoids
    • Recovery of organoids for downstream applications

    Speaker Bio:

    Dr. Nitin Kulkarni is a member of the Scientific Support team at Corning Life Sciences. He has a Ph.D. in Biology and has worked on engineering transgenic mouse models for autoimmune diseases during his post-doctoral research at the Beth Israel Deaconess Medical Center in Boston, MA.
    In his current role, he supports researchers with applications related to cell culture including advanced surfaces and extracellular matrices, genomics, drug discovery and bioprocesses.
  • Rescheduled: Multicellular Tumor Spheroids in HTS: New Assays Recorded: Feb 1 2017 50 mins
    Wojciech Senkowski
    Three-dimensional cell cultures, and multicellular tumor spheroids in (MCTS) in particular, have recently become a widely used tool for preclinical anticancer drug testing in high-throughput screening (HTS) setup. However, even though MCTS have been applied for HTS, their use has been limited to simple assays, such as assessing cell viability or inhibition of growth.

    This webinar will cover new approaches to MCTS-based HTS. It will present a new, robust viability assay, well-suited for HTS and based on green fluorescent protein (GFP) used as a surrogate marker of spheroid viability. It will also review a first-ever approach to obtain information-rich transcriptomic data from drug-treated MCTS on a large scale. In addition, the presenter will demonstrate how this novel platform resulted in the identification of previously unrecognized, context-dependent drug responses of cancer cells and in findings with potential clinical relevance.

    In summary, this webinar will demonstrate new ways of how MCTS-based HTS can be used to provide unique insights into context-dependent biology and cellular drug responses.

    About the Presenter:

    Wojciech Senkowski will soon complete his Ph.D. in Medical Sciences at Uppsala University, Sweden. In his work, he looks for applications of various tumor spheroid models in high throughput drug screening. For his work, Wojciech has received the AACR Scholar-in-Training Award. He was also a presenter and expert panelist at the Genetic Engineering & Biotechnology News webinar on 3D cell cultures, sponsored by Corning in February of 2016.
  • The Impact of Soluble Factors and Substrate on Cell Culture Recorded: Nov 3 2016 48 mins
    Kevin Kelly
    Webinar: The Impact of Soluble Factors and Substrate on Cell Culture: Media Additives, Growth Factors, and Surface

    From basal media with feeder layers or serum to highly defined recombinant growth factors, cytokine, and extracellular matrix, there are many ways to grow the same cell type. The choice is dependent on scale, cost, control, skill, and regulatory factors.

    This webinar will cover:
    - Different ways to grow the same cell type
    - The actual material costs of various methods
    - Methods used to optimize formulations

    Speaker Bio:
    Kevin Kelly graduated from Hawaii Pacific University and for 15 years worked on process scale-up and optimization for extracellular matrix proteins, growth factors, cytokines, antibodies, ELISA kits, and Corning® BioCoat™ products.

    Currently he provides applications support for invasion, migration, permeability, transport, differentiation, and metabolism assays.
  • Current Trends in 3D and Organoid Cell Culture for Cancer Research Recorded: Oct 4 2016 67 mins
    Marshall Kosovsky, Ph.D., Ömer H. Yilmaz, M.D., Ph.D.,Wojciech Senkowski,
    Corning was pleased to have recently sponsored a GEN webinar highlighting the latest techniques for 3D cell culture in cancer research.

    The use of 3D cell cultures has been rising sharply in recent years from its initial introduction, over two decades ago. Because 3D cultures more accurately mimic the cellular environment, they can be used to study various forms of cancer by fostering the growth of organoids that replicate key properties of in vivo organ systems or the original tumors from which they were derived.

    In addition, many 3D cultures are amenable to large-scale drug screens for rapid detection of phenotypic or genetic changes associated with therapeutic compounds—an approach that opens the door for the use of 3D culture as an integral part of personalized medicine.

    In this GEN webinar, panelists discussed how the latest 3D cell culture methods have facilitated breakthroughs in their research projects.

    Panelists:
    Marshall Kosovsky, Ph.D., Global Scientific Support Manager for Corning Life Sciences, will give a brief introduction into advances in Corning’s 3D culturing solutions.

    Ömer H. Yilmaz, M.D., Ph.D., Assistant Professor of Biology at the Massachusetts Institute of Technology, will describe his work on how adult stem cells and their microenvironment adapt to diverse conditions within the context of tissue regeneration and cancer initiation through the use of ex vivo intestinal organoid assays.

    Wojciech Senkowski, Doctoral candidate in the Department of Medical Sciences at Uppsala University in Sweden, will discuss his current work, which looks for applications of various tumor spheroid models in high-throughput drug screening for ways to identify novel compounds that target these cell populations
  • Using Microcarriers to Speed-up Your Scale-up. Recorded: Sep 15 2016 33 mins
    Jennifer Weber
    This presentation describes critical factors for selecting microcarriers, as well as starting protocols to help you optimize the attachment and expansion of cells in spinner flask and bioreactor environments.

    In addition, you’ll learn about new dissolvable microcarrier technology, which provides unique advantages for cells that cannot be easily separated from standard microcarriers.

    Speaker Bio:

    Jennifer Weber is a senior development scientist with Corning Life Sciences. She has helped develop a variety of products for culturing advanced cell types including Corning® Synthemax™, a synthetic, xeno-free surface, and Corning stemgro ® hMSC, a serum-free, chemically defined medium for hMSC culture.

    She recently transitioned to microcarrier product development and customer support for bioprocess applications. As part of this role, she facilitates customer adoption of Corning products for specific applications through protocol development, on-site technical support, and in-house customer-driven projects.
  • Understanding Cell Culture Media Composition to Improve Outcomes Recorded: Jul 28 2016 59 mins
    Brian Posey
    Cell culture media is required for successful and reproducible research but the catalog is full of acronyms and various formulation tables. Classical mammalian cell culture media formulations are very diverse both in terms of the number available and the concentration of constituents. Additionally, each medium was designed for specific cell types and culturing conditions.

    This webinar will cover:
    •The composition, characteristics, environmental factors, and additional supplements required to create optimal conditions for growth and productivity.
    •Determining the right formulation for your application.
    •Serum usage and helpful tips for optimizing your culture conditions.

    Speaker Bio:
    Brian Posey is a Product Development Manager for cell culture media at Corning Life Sciences. Brian has over 10 years experience in cell biology and industrial scale cGMP manufacturing of both liquid and powder cell culture media. Since joining Corning in 2012, Brian has led numerous innovative technology projects for the media business ranging from customer technology transfer for production scale-up to developing new serum-free media for industrial and stem cell lines.
  • In Vitro Characterization of Species Difference of OATP Recorded: May 25 2016 52 mins
    Na Li, Ph.D.
    Organic anion-transporting polypeptides (OATPs) play an important role in hepatic uptake of a variety of clinically important drugs. The significant differences in OATP/Oatp-dependent drug transport between human and preclinical species presents a challenge for interspecies extrapolation of drug pharmacodynamics and pharmacokinetics. The assessment of the difference in hepatic uptake between species using an in vitro model is highly desired to support mechanistic studies and to understand the differences observed between species in vivo.

    The Corning® TransportoCells™ model has demonstrated significant value in terms of supporting in vitro assessment of drug interaction with SLC transporters in drug discovery and development. Recently, several animal species of Oatps were introduced into the Corning TransportoCells portfolio. This webinar will focus on the development of the newly available animal species and characterization of the differences in OATP/Oatp in substrate specificity and kinetics using this "thaw and go" model.
  • Specialized Applications Using the Corning® Spheroid Microplate Recorded: Apr 22 2016 47 mins
    Hilary Sherman, Applications Specialist & Audrey Bergeron, Application Specialist
    Three-dimensional (3D) cell culture models, which offer significant improvements over traditional two dimensional monolayer cell culture in terms of maintaining morphological and functional characteristics of tissue, are increasingly being incorporated in drug discovery as model systems to study disease or for screening for chemotherapeutic efficacy or drug toxicity. This presentation will demonstrate the use of Corning® spheroid microplates to culture and assay spheroids in a rapid and highly reproducible format that enables the formation of a single multi-cellular spheroid, centered in each well.

    Corning spheroid microplates are multiple well, cell culture plates with opaque walls and unique clear, round well-bottom geometry that utilize the Corning Ultra-Low Attachment surface coating. The coating is hydrophilic, biologically inert and non-degradable. Representative data will be shown exhibiting the use of the spheroid microplate in more specialized assays including the formation of neurospheres from neural stem cells (NSCs), a valuable model to study neurogenesis and neural development, as well as analysis of NSC proliferation and migration. We will also demonstrate the formation of hepatospheres using Corning HepatoCells, an immortalized alternative to primary human hepatocytes, in combination with the SCREEN-WELL Hepatotoxicity library from Enzo Life Sciences for 3D hepatotoxicity screening. Finally, we will show the use of the Corning spheroid microplate to generate spheroids comprised of multiple cell types, demonstrating the impact that including multiple cell types in 3D assays can have on therapeutic outcome in a chemotherapeutic assay.
  • There’s More to Choosing a Filter Than You Might Think Recorded: Feb 25 2016 54 mins
    Mark Rothenberg, Ph.D.
    Although filtration is commonly practiced in many laboratories, it is a critical step in preparing media and buffers, clarifying samples, removing microbial contaminants, concentrating and inline filtering gases, to name a few applications. In order to choose the correct filter for your application, you need to consider the application, the material(s) to be filtered, and other factors that will be covered in this presentation. This webinar will focus on:

    An Introduction to Filtration:
    • Basic principles of filtration
    • Overview of membranes
    • Overview of formats

    Choosing the Correct Filtration Product for your Application:
    • Sterilization
    • Clarification of solutions
    • Ultracentrifugation

    About our Presenter:

    Dr. Mark Rothenberg graduated from Emory University with his Ph.D. in Cell and Developmental Biology. Over the past 25 years, Mark has held positions in both academia and industry where he has developed an expertise in the areas of assay development and cell culture. He currently holds the position of Manager Scientific Training and Education with Corning Life Sciences.
  • A New SLC Transporter Model to Study the Impact of OATP1B1 Genetic Variants Recorded: Nov 16 2015 48 mins
    Na Li, Ph.D.
    The importance of genetic variations in drug transporters for drug disposition and response has been increasingly recognized in the past decade. The drug transporter organic anion transporting polypeptide 1B1 (OATP1B1) is genetically polymorphic and plays a major role in hepatic uptake of a variety of clinically important drugs. Predicting the pharmacokinetic effect of these genetic variants on the drug disposition is critical for understanding the inter-individual variations to drug efficacy and safety. Corning TransportoCells was introduced to support in vitro assessment of drug interaction with SLC transporter. Specially, the newly developed TransportoCells OATP1B1 SNPs, alongside with OATP1B1 wild type, offer an in vitro tool kit for studying the potential impact of genetic variants on drug PK.

    This webinar will focus on the development and characterization of the newly available TransportoCells OATP1B1 genetic variants, three animal species Oatp family members and other drug transporters of emerging importance in drug development.
  • Corning® HepatoCells: A Novel Cell-based Model for In Vitro ADME/Tox Studies Recorded: Oct 7 2015 49 mins
    Rongjun, Zuo, Ph.D.
    Primary human hepatocytes (PHHs) and other hepatic cell models possess several limitations. For instance, PHH’s large lot-to-lot variation requires qualification tests with each lot, resulting in high costs and increased lead time. Furthermore, other non-primary hepatic cells can have insufficient fold induction in some lots and conditions.

    This presentation will introduce Corning® HepatoCells for ADME/Tox studies. Derived from primary human hepatocytes, Corning HepatoCells are a renewable, hepatocyte-like cell line that retains most of the physiological properties of their parental hepatocytes such as mature hepatocyte-like morphology and induction response to prototypical inducers of CYP3A4, 1A2, and 2B6. Characterization of Corning HepatoCells for ADME/Tox studies will be presented, along with data demonstrating how the model system can be used for prediction of clinical CYP induction.
  • Your In Vitro ADME CRO: New Assays for Transport, Enzyme Inhibition & Induction Recorded: Jul 29 2015 60 mins
    David M. Stresser, Ph.D.
    Achieving your ADME/Tox testing goals requires experience, quality data, and proper alignment with regulatory guidance. Failure to meet these important requirements can put your drug discovery and pre-clinical goals at risk.
    This presentation will provide an informative overview of how you can advance and reach your pre-clinical drug discovery goals. It will discuss the importance of core contract research capabilities, including enzyme induction, enzyme inhibition, and transporter interaction. In addition, we will review new capabilities and opportunities including CYP induction and SLC transporter assay services – all designed to align with regulatory agency guidance documents.

    Speaker Bio:
    David Stresser is the Program Manager of Corning® Gentest℠ Contract Research Services at Corning Life Sciences since 2001, having held prior positions of Product Manager and Study Director since joining Corning in 1998. Prior to this, he was a post-doctoral associate in the laboratory of David Kupfer at the University of Massachusetts Medical School in Worcester, Massachusetts. He did his graduate work in the laboratory of David E. Williams at Oregon State University in Corvallis, Oregon receiving a Ph.D. in toxicology in 1994. Dr. Stresser has authored or co-authored 40 articles or book chapters in the field of drug metabolism and has been an invited speaker at various national and international meetings, pharmaceutical companies, and universities.
  • Authentication of Cell Lines for Pre-Clinical Cancer Research Recorded: Jun 22 2015 58 mins
    Yvonne A. Reid, Ph.D.
    Join us on June 22nd for a special Corning-sponsored webinar presented by ATCC®.

    Abstract:
    Animal cell lines are important in vitro systems and tools for scientists in diverse disciplines such as basic cell biology, genetic mapping, gene expression and gene therapy. Cell line authentication and characterization are crucial in these activities, yet they are underappreciated by most research scientists. Over the years numerous cell lines have been shown to be misidentified due, in part, to poor techniques and inadequate verification of cell line authenticity. Technological advances have given rise to improved capabilities. Cell line authentication now requires a comprehensive strategy that employs several complementary technologies for systematic testing for morphology, microbial contaminations, cellular identity/cross-contamination as well as functionality. The validity of conclusions drawn from research data is dependent on consistent and unequivocal verification of cell line identity and function. It is estimated that the financial loss incurred by poorly characterized or misidentified cell lines is in the millions of dollars. An overview of the current technologies used to authenticate cell lines will be presented.

    Speaker Bio:
    Dr. Yvonne A. Reid joined ATCC in 1980 and during the mid-1980’s her research focused on the use of DNA hypervariable probes for the intraspecies identification of cell lines. The evolution of this work has led to the implementation of routine screening of all human cell lines by STR analysis. She co-chaired the ATCC SDO committee on the Development Consensus Standard on the Authentication of Human Cell Lines: Standardization of STR profiling. Dr. Reid has more than 30 years of experience in cell biology, immunology and molecular biology. As Collection Scientist for the Cell Biology Program for over 10 years, she was responsible for acquisition of new animal cell lines and hybridomas into the Cell Biology General Collection.
  • An Introduction To Cell Culture Recorded: Apr 30 2015 58 mins
    Mark Rothenberg, Ph.D.
    This webinar will introduce the history, theory, basic techniques, and potential pit-falls of mammalian cell culture. It is designed for students and new lab technicians, as well as bench scientists interested in updating their techniques or knowledge in the field.

    Topics to be discussed include:
    • History and practical theories of cell culture and its impact on today’s science
    • The requirements needed to set up a cell culture laboratory
    • Challenges when performing mammalian cell culture and how to overcome them

    About our Presenter:
    Dr. Mark Rothenberg graduated from Emory University with his Ph.D. in Cell and Developmental Biology. Over the past 25 years, Mark has held positions in both academia and industry where he has developed an expertise in the areas of assay development and cell culture. He currently holds the position of Manager Scientific Training and Education with Corning Life Sciences.
  • Tips and Techniques for Serum-free Expansion of hMSCs Recorded: Mar 26 2015 53 mins
    Brian Posey
    Abstract:
    There is a great interest in application of human mesenchymal stem cells (hMSCs) in cell therapy and tissue engineering due to their self-renewal, multi-lineage differentiation, immunomodulation, and trophic potential. One of the challenges faced in the clinical application of hMSCs is the need for efficient expansion of these cells in vitro without altering their capacity. Serum-free mammalian cell culture media, in particular, require optimization of the expansion protocols. Even subtle changes in routine handling can have a significant impact on the cells’ potential.

    This seminar will cover the variables that can influence the desired regenerative and differentiation properties including medium selection, vessel surface treatment, impact of the cell source, and seeding density. We will also discuss how users can select the correct conditions for optimized growth and functionality.

    Speaker Biography:
    Brian Posey is a Product Development Manager for cell culture media at Corning Life Sciences. Brian has over 10 years experience in cell biology and industrial scale cGMP manufacturing of both liquid and powder cell culture media. Since joining Corning in 2012, Brian has lead numerous innovative technology projects for the media business ranging from customer technology transfer for production scale-up to developing new serum-free media for industrial and stem cell lines.
Training for Life Science Researchers
The Corning Scientific Seminar Series is a series of free, online technical presentations that provide novel tips, best practices and proven techniques to help advance your research. Delivered by scientists to scientists, these one-hour broadcasts offer useful information and tips for lab technicians and researchers.

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  • Live at: Feb 10 2011 3:10 pm
  • Presented by: John So, M.S.
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