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    • Panel Discussion: Innovation in Medtech
      Panel Discussion: Innovation in Medtech Pengson Ji (Chairman Ankon Technologies), Jingwei Lou (Chairman Biotecan Biology) Recorded: Jul 25 2017 9:00 am UTC 29 mins
    • Innovations in MedTech are playing an important role in China's economic restructuring. MedTech is undoubtedly one of the key sectors expected to be at the forefront of strategic technological progress in the coming years. Revolutionary potential lies in the conception and development of devices such as new imaging equipment and medical robots, in addition to the enhancement of biological 3D printing techniques.

      Due to factors such as an ageing population and greater personal consumption, China will continue to see the MedTech industry thrive. According to data from the China Medical Pharmaceutical Material Association (CMPMA), the Chinese medical equipment market has grown rapidly from just RMB 14.5 billion in 2004 to RMB 255 billion in 2014, representing a 16-fold increase in the short space of 10 years. The market is expected to exceed RMB 300 billion this year thanks to the effect of the ‘Made in China 2025’ strategy.

      Led by the panel moderator Tina Tan (Editor-in-Chief of Medtech insight), the panelists discussing such transformations are Jingwei Lou (Chairman, Biotecan Biology), Neil White (Head of Life Sciences of Medopadand) and Shibing Xia (Shibing Xia, General Manager at Golden Sunflower Capital Management).

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    • Literature search on a connected path
      Literature search on a connected path Elaine Reynolds, Assoc Prof of Biology & Neuroscience, Lafayette College; Rick Misra, PhD, ScienceDirect Product Manager Recorded: Sep 29 2017 3:00 pm UTC 54 mins
    • "What if researchers — particularly those new to certain disciplines or to research itself — could have a Wikipedia-like experience that was streamlined and trusted?" This is the question that led two neuroscientists on a fascinating journey to improve literature search, from exploring users' needs to applying cutting edge technologies.

      Students in an introductory and a higher-level class were assessed to determine where they were doing their research, their comfort level with reading reviews and primary literature, how frequently they came across unfamiliar terms, and how they handled cases where they needed additional clarity. Then they were provided the beta version of ScienceDirect Topics, an enhancement to the database that provides links within journal articles to 80,000+ topic pages with citable and trusted definitions that are contextualized within a discipline.

      Did it make a difference? Find out by attending the Library Connect webinar: Literature search on a connected path.

      Registration is required for this live one-hour webinar. It will be broadcast internationally and includes time to ask the presenters questions during the session. The webinar is a complimentary event and part of Elsevier's Library Connect program for academic, medical, corporate and government librarians.

      Cannot attend on Sept 29? Register for the webinar and you will be notified when it can be viewed online after the event.

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    • Generation of a landing-pad T cell line useful for T cell receptor customization
      Generation of a landing-pad T cell line useful for T cell receptor customization Stacey Ward, PhD Upcoming: Apr 17 2018 5:00 pm UTC 75 mins
    • T cell biology is integral to the study of normal immune regulation as well as cancer biology, Car-T cells, epitope specificity and antigen presentation. However, primary T cells can be difficult to propagate in culture for the length of time necessary for functional assays. In addition, primary T cells express variant T cell receptor (TCR) heterodimers that can be challenging to identify and may not be optimal for downstream studies. We sought to simplify this system using transformed T cells which can be grown in culture for extended periods of time. We engineered a floxed landing pad sequence into the safe harbor AAVS1 genetic locus using CompoZr zinc finger nucleases. Both the promoter and landing pad expression cassette are flanked by unique lox sites, allowing swapping of either the promoter and/or expression cassette as needed. We ensured that only one copy of this sequence was found within the genome to avoid any complications associated with random insertion events. We also generated a landing pad cell line null for the endogenous TCR using Cas9/CRISPR ribonucleotide complexes. Both the TCR alpha and beta loci were rendered null due to non-homologous end joining and the presence of insertions and deletions culminating in premature stop codons were genotyped using next generation sequencing. The absence of a functional TCR was validated using flow cytometry staining for surface TCR and CD3. This cell line was then used to generate a knock-in of the desired exogenous TCR heterodimer to the landing pad locus, verified using flow cytometry staining. These lines will be very useful for a multitude of studies where a researcher needs to express a gene of interest in a discrete genetic locus or wants to generate a panel of TCR expressing cell lines.

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    • Amplified Detection of Proteins and their Interactions using Duolink PLA
      Amplified Detection of Proteins and their Interactions using Duolink PLA Manpreet Mutneja, Ph.D, MBA Sr. Product Manager, Molecular Platforms Life Science Research, MilliporeSigma Recorded: Jun 15 2017 5:00 pm UTC 44 mins
    • Understanding the movements, modifications and interactions of proteins within a cell is key to unraveling the fundamental tenets of biology. However, the low-level expression of many proteins, combined with the transient nature of their interactions and movements, makes analyzing and understanding these processes quite difficult. Duolink® PLA, which is based on the principles of the proximity ligation assay (PLA), offers a solution to overcome these hurdles and to study the actions of endogenous proteins within cells and tissues. Combining the specificity of antibodies with the sensitivity afforded by rolling circle amplification, Duolink® PLA allows you to detect, visualize, and quantitate proteins and their interactions (even single events) where they happen within cells or tissue, all without overexpression or genetic manipulation. This seminar will cover the basic assay principle and advantages of the Duolink® PLA technology, and discuss recent applications and developments of the technology that make it an excellent tool to understand the fundamental mechanisms of biology, as well as disease states. Applications of Duolink® PLA include the investigation of cellular responses to varying stimuli, receptor dimerization and signalling cascades, post-translational modifications, and regulation of protein expression. New developments include use in flow cytometry and multiplexed detection.

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    • The Laboratory’s Role in the National Healthcare Safety Network
      The Laboratory’s Role in the National Healthcare Safety Network Robert L. Sautter, Ph.D., HCLD (ABB), CC Upcoming: Apr 9 2018 6:00 pm UTC 60 mins
    • In this webinar, Dr. Sautter will discuss the important collaboration efforts between the government and healthcare facilities to stop prevalent and dangerous hospital-acquired infections. After the webinar, you will be able to:

      • Explain how the laboratory and the NHSN can work together to lower infection rates in hospitals
      • Learn how to lower rates of infections, such as blood culture contamination, MRSA and C. difficile
      • Identify how pre-analytic culture collection can affect the results of clean catch and catheter-related infections
      • Discuss how laboratories can work together with the NHSN, an infection surveillance program created by the CDC to eliminate HAIs, to identify problem areas and measure the progress of prevention initiatives

      P.A.C.E. credit is available for your participation.*

      Dr. Sautter is a teacher, lecturer and industry consultant. He earned his B.S. and M.S. degrees from Eastern Michigan University in the areas of biology and molecular biology, respectively, and his Ph.D., from Wayne State University, in Detroit, Mich., in microbiology. During his career, he held positions as a medical technologist, director of microbiology and medical director for a number of laboratories, before becoming an esteemed consultant for a variety of industry leaders in the area of microbiology.

      *Beckman Coulter Inc. is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E.® Program. These credits are recognized by the State of California. Most programs also provide State of Florida credits (with valid license number). At this time, we cannot issue continuing education credits for those who provide healthcare (or work for an institution that provides healthcare) in Massachusetts or Vermont.

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    • Modeling NAFLD and TGFβ-induced Fibrosis in 3D Bioprinted Human Liver Tissue
      Modeling NAFLD and TGFβ-induced Fibrosis in 3D Bioprinted Human Liver Tissue Jeff Irelan, Ph.D. Recorded: Oct 5 2017 4:00 pm UTC 52 mins
    • We hope you join us for this special webinar. Jeff Irelan, Director of Scientific Applications for Organovo will be our guest presenter.

      Nonalcoholic fatty liver disease (NAFLD) is the most common liver disorder with an estimated prevalence of over 25% worldwide and is projected to become the leading indication for liver transplant by 2025. Despite decades of research focused on NAFLD, an effective treatment has yet to be approved. This is due in part to the reliance on cell culture and animal models that present challenges in translation due to limited functional longevity and species differences, respectively.

      ExVive™ 3D Bioprinted Human Liver Tissue, a clinically-translatable in vitro model, is ideal for studying the effects of drugs on liver disease progression, regression, and the mechanisms involved. Here, we present results showing a nutrient overload induction of liver disease and TGFβ-induced fibrosis in ExVive™ Human Liver Tissue. A variety of disease-relevant phenotypes including steatosis, inflammation, and fibrosis can be demonstrated in the model:
      •Nutrient overload leads to the accumulation of lipid droplets in hepatocytes.
      •Incorporation of Kupffer cells and stimulation induces inflammatory cytokine release.
      •Chronic exposure to nutrient overload leads to stellate cell activation and fibrosis.
      •Chronic exposure to chemical inducers of fibrosis or TGFβ stimulation leads to stellate cell activation and fibrosis.
      •A TGFβR1 kinase inhibitor effectively blocks TGFβ-induced fibrosis.

      Presenter Bio:
      Jeff Irelan holds a Ph.D. in molecular biology from the University of Oregon. As Director of Scientific Applications, Jeff interfaces with Organovo’s customers and R&D team to implement and expand the company’s portfolio of service offerings utilizing bioprinted tissue models.

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    • Developing Novel Microfluidic Devices with Multiphysics Simulation
      Developing Novel Microfluidic Devices with Multiphysics Simulation Robyn Pritchard, Consultant Physicist The Technology Partnership (TTP) Andrew Young, Technical Manager COMSOL Upcoming: Apr 25 2018 1:00 pm UTC 60 mins
    • If you are interested in using simulation to enhance the development of a microfluidic device, then tune into this webinar with Robyn Pritchard from The Technology Partnership (TTP).

      Cell sorting is a staple of many cell biology labs. The current gold standard, fluorescence-activated cell sorting (FACS), is limited to processing roughly 10,000 cells per second to avoid irreparable damage. For many new advances in cell therapy (e.g., autologous T-cell therapy for cancer), throughput of up to a billion cells in a few hours is essential. TTP set out to break through the current limit by developing a novel microfluidic cell sorter.

      In this webinar, Robyn will discuss how TTP used the COMSOL Multiphysics® software to design and invent the world’s smallest ultrafast microfluidic cell sorter, where the key discovery was the inertial vortex. Robyn will also show how TTP's simulations correspond to reality with their fully working microfluidic chip.

      The webinar will include a live demonstration and conclude with a Q&A session.

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    • Surfaces for Organoid Culture
      Surfaces for Organoid Culture Nitin Kulkarni, Ph.D. Recorded: Feb 23 2017 5:00 pm UTC 50 mins
    • 3D culture is gaining pivotal importance for attaining in vivo-like conditions in a dish to study developmental cues as well as therapeutic possibilities. Organoid development promises to be one of the most important research tools in the near future. This presentation will cover:

      • Methodologies used in organoid culture
      • Matrices for growing organoids
      • Recovery of organoids for downstream applications

      Speaker Bio:

      Dr. Nitin Kulkarni is a member of the Scientific Support team at Corning Life Sciences. He has a Ph.D. in Biology and has worked on engineering transgenic mouse models for autoimmune diseases during his post-doctoral research at the Beth Israel Deaconess Medical Center in Boston, MA.
      In his current role, he supports researchers with applications related to cell culture including advanced surfaces and extracellular matrices, genomics, drug discovery and bioprocesses.

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    • An introduction to best practices for refereeing a journal manuscript
      An introduction to best practices for refereeing a journal manuscript Professor Simon Cherry, University of California, Davis, USA Recorded: Feb 13 2018 4:00 pm UTC 45 mins
    • Peer review is one of the most critical components of high-quality scientific publishing. It impacts on the timeliness, accuracy and clarity of research findings that—if published—will be accessible and visible to everyone for years to come.

      At its best, the peer-review process establishes whether the work makes a genuine and valuable contribution to the literature, and can remove errors and help authors to better explain their results and the significance of their work to the broad audience who may read the final journal article. At its worst, however, inadequate or superficial peer review can allow substandard work or poorly explained research to be published, which is to the detriment of the scientific enterprise. In addition, the peer-review process can also impede the publication of high-quality work through unnecessary delays or personal bias.

      The goal of this webinar is to address best practices for reviewing a journal manuscript and address some of the challenges and pitfalls that frequently crop up during the peer review process. This webinar is aimed at those new to serving as a referee for a scientific journal – whether that be more generally, within the field of medical physics, or for Physics in Medicine & Biology specifically.

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    • Designing the Next Phase in Genome Editing: Advanced CRISPR Applications
      Designing the Next Phase in Genome Editing: Advanced CRISPR Applications Jeremy Lehmann- CRISPR Product Specialist, Functional Genomics, MilliporeSigma Recorded: Mar 1 2016 6:00 pm UTC 44 mins
    • CRISPR Cas9 nucleases have revolutionized the field of genome editing enabling unprecedented efficiency of gene targeting in a vast array of cell types and organisms. Even with such powerful technology at hand, researchers who are new to the field may find genome modification to be challenging and time-consuming. As CRISPR becomes a focus of the molecular biology research community, MilliporeSigma seeks to share the best approaches learned and methods applied in our years of genome editing experience. Today’s presentation will focus on practical applications of CRISPR for pristine genome editing to achieve knockout as well as specific sequence changes to include donor-mediated snps, reporter-tags and conditional knockouts. Special attention will be paid to design considerations for the donor constructs necessary to achieve specific sequence changes. Finally, the frontiers of CRISPR technology, including synthetic crRNA to fast-track genome editing experiments, whole genome screening and targeted gene activation will be explored.

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