Despite our dependence on cultured cells as models, issues surrounding cell identity, choice of appropriate cell lines, conditions of their growth and storage, microbial contamination and over-passaging are often overlooked or misunderstood. Mistakes can have devastating impacts on the legitimacy and reproducibility of science but potentially also, on careers, reputations and funding streams.
Solutions and options for managing cell culture processes
The Impact of misidentification
The international consensus on cell identity and qualification
What’s in your vial? Best practices for maintaining microbial QC strains.
Director, Standards and Certification,
Microbiology, Biodefense and Emerging Infectious Diseases
Jaspreet Sidhu, Ph.D.
Vice President of Business Development and Pharmaceutical Microbiology
Molecular Epidemiology, Inc.
In the pharmaceutical and personal care industries, products, processes and environments are quality control tested to prevent microbial contamination. Microbial strains with confirmed identity, viability and purity, produced by meticulous laboratory procedures that minimize subculturing, are important components of quality control testing programs. Responding to industry demands for rapid microbiological testing, instrumentation and associated databases have been developed that allow for the standardized testing of phenotypic and genotypic traits across a wide array of microorganisms. A polyphasic approach to identification provides strain confirmation and avoids the pitfalls of misidentification, painful recalls and regulatory repercussions. Case studies will be provided to illustrate key points.
Major Learning Objectives:
•Understanding the importance of polyphasic microbial identification
•Best practices for maintaining the stability and integrity of microbial QC strains.
•Importance of confirming microbial identification – pitfalls of misidentification.