Daniel Mahony, manager of Polar Capital Global Healthcare Growth & Income discusses the outlook for the Biotechnology and Healthcare sector.Read more >
Hydrophilic interaction liquid chromatography (HILIC), especially in conjunction with mass spectrometry (MS), has become a powerful tool for the analysis of a wide variety of challenging analytes. Applications of the technique have increased dramatically over the past decade, especially for the analysis of polar analytes where reversed-phase chromatography suffers. HILIC conditions employ a high percentage of acetonitrile which enables facilitated solvent evaporation in LC/MS sources and thus often an increase in analyte response when compared to more aqueous based systems. The increased retention of polar analytes afforded by HILIC provides improved selectivity and decreases the impact of endogenous species, often leading to improved qualitative and quantitative analyses.
Although HILIC has proven useful, it has also been thwarted with complications including difficulties in method development and method robustness.
In this presentation, studies investigating the underlying retention mechanisms dominant in HILIC chromatography are presented and discussed. Along with reversed-partitioning HILIC is well known to exhibit, ion-exchange and the interplay of the dominant mechanisms are unveiled and used to develop a model of overall retention and selectivity. Interactions that operate using different stationary phase chemistries and conditions are presented. The impact of analyte polarity and charge as well as the variations caused by high percentages of organic on these physiochemical parameters are highlighted. Throughout the discussion, examples of use and misuse of HILIC are employed to illustrate these important concepts to build a solid fundamental foundation for efficient and effective use of this powerful technique.
On the surface, ClearCase and Git seem like polar opposites, yet setting architecture aside, they have interesting similarities. A deeper understanding of ClearCase and Git will ease the migration planning process.
Register for this webinar to learn:
- How Git and ClearCase differ and how they support some of the same development models
- Options for moving data into Git with low technical risk
- How ClearCase MultiSite, ClearCase UCM, and ClearQuest fit into the picture
- ...and more!
Divergence remains a strong theme in this multi-polar world.
In its annual Tech Heads study, Octopus Group spoke to over 400 UK directors to explore trends in content consumption and buyer behaviours for B2B businesses. (To view the full Tech Heads report, visit: http://bit.ly/TechHeads15)
The findings reveal a paradox: attention spans are shortening, but decision making time is extending (an average of 16.5 weeks currently according to respondents).
Octopus Group calls this challenge the ‘Content Pinch Point’, and it’s a big problem for marketers. It means that it’s never been harder to connect with and subsequently convert prospects.
Marketers now face a difficult task: catching prospects’ attention quickly through bold, creative, differentiating campaigns that are short on words and big on impact; yet once sales teams are engaged with prospects, marketing needs to suddenly become considered, consultative and big on substance to help their sales colleagues get over the finishing line.
Two very different styles of content marketing, which are incredibly difficult to integrate without appearing to have a bi-polar brand.
But there’s a huge opportunity for marketers who do manage to get it right, as their brand will give prospects a truly elevated and differentiated experience, which will tell in the pipeline they generate.
This webinar aims to help marketers capitalise on the opportunity and Billy Hamilton-Stent, Octopus Group’s client strategy director, will cover:
How the content ‘pinch point’ is adversely affecting marketing departments.
The tell-tale signs that reveal the pinch in your campaigns.
Strategies to rectify the problem and turn results around.
Practical ways marketers can support sales in the journey from interested lead to high value customer.
Click here to view Tech Heads: http://bit.ly/TechHeads15
How concerned is your organisation about adverse weather? Recent polar vortexes and flooding across the North America and Europe have highlighted once again that Mother Nature can cause significant disruption and let us not forget the utter devastation that typhoon Haiyan caused the Philippines in November 2013. Understanding the risk and impact to your organisation before an incident occurs is critical to your resiliency. Join us for this webinar to participate in live polling, discuss industry research and understand how we can all benefit from adopting best practice principles to mitigate the risk.Read more >
We review the latest developments in investment trusts - new launches, new issuance, funds closing and restructuring. We look at the bull and bear cases for the different trusts - and how they can serve a range of investor needs - income, diversity, growth.
The panel will also broaden this out into a wider discussion of the current market environment and how new issuance meets the needs of that environment - for example, the spike in bond yields, or the improving equity markets.
Moderator: Jackie Beard, Morningstar UK
John Yakas, Polar Capital
Robert Worthington, J.P. Morgan Asset Management
Glycols are particularly difficult to extract from water because traditional methods like LLE and standard SPE phases fail due to low recovery. ENVI-Carb Plus extraction results in high retention/recovery and better sensitivity for glycols by GC analysis.Read more >
Increasing case loads and budget and staffing cuts in forensic laboratories continue to motivate the development of higher throughput methods, particularly for confirmatory analysis of regulated intoxicants. In this work, we have focused on the development of rapid LC/MS/MS methods for the determination of nine opiates including two glucuronide metabolites, and 16 benzodiazepines, including two amino- metabolites. Here we aim to analyze both the parent compounds and important polar metabolites in a single analysis. To this end we have compared the retention of the target compounds on two different reversed-phase HPLC stationary phases: a conventional C18 type phase, and a perfluorinated phenyl (PFP or F5) phase built upon the increasingly popular Fused-Core particle morphology. We see that the F5 phase not only generally exhibits higher retention than the C18 type phase, but also exhibits very different selectivity such that the nine opiates can be nearly completely resolved in under four minutes. We find that the mixture of 16 benzodiazepines cannot be completely resolved in a reasonable (i.e., less than 20 min.) time, however we have developed a separation with no more than three overlapping peaks in an analysis time of five minutes.
DEVELOPMENT OF RAPID LC/MS/MS-BASED METHODS FOR CONFIRMATORY ANALYSIS OF OPIATES AND BENZODIAZEPINES
SPENCER BONNERUP, D. CHRISTOPHER HARMES, TOMAS LISKUTIN, JONNA BERRY, AND DWIGHT R. STOLL
Department of Chemistry
Gustavus Adolphus College
800 West College Avenue
St. Peter, MN 56082