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    • Characterisation of Host-Cell Proteins using Mass Spectrometry Enables Effective
      Characterisation of Host-Cell Proteins using Mass Spectrometry Enables Effective Dr Li Zang Upcoming: Oct 31 2017 2:00 pm UTC 75 mins
    • Common mammalian cell lines used for biopharmaceutical production include Chinese Hamster Ovary (CHO), NS0 and Human Embryonic Kidney (HEK) cells. Each of these cell lines has been found with over 20,000 genes coded in their genome, which can result in over 10,000 proteins expressed at the same time in these cells. These proteins can be secreted from the living host cells or released to the cell culture supernatant upon lysis of the host cells during the cell culture. Biopharmaceuticals produced using these cell lines can be co-purified with a subset of the host-cell proteins (HCPs) in the cell culture supernatant.

      These co-purified HCPs are considered process-related impurities for biopharmaceuticals. The HCPs can cause potential safety risks by introducing anti-HCP response in the patients. Depending on the biological functions of the residual HCPs, other potential impacts reported include lowering the biopharmaceutical protein stability and affecting the efficacy of the biopharmaceutical protein by exacerbating the symptoms.

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    • Improve Your Mass Spectrometry Workflows With the New NIST Software
      Improve Your Mass Spectrometry Workflows With the New NIST Software Prof David Sparkman Recorded: Apr 23 2015 1:30 pm UTC 77 mins
    • Improve Your Mass Spectrometry Workflows With the New NIST Software, Featuring the Wiley 10th/NIST 2014

      Hosted by Prof David Sparkman

      Release 2.2 is one of the most significant improvements in NIST’s MS Search software. Prof Sparkman will lead an online webinar providing insight into the latest improvements in the most popular mass spectrometry program on the planet, with insights into how you can improve and speed up your analyses by using the full suite of functions available in MS Search 2.2 and the power of using these with the broadest compound coverage available.
      -Compatibility and systems interface tips
      -Retention time workflow
      -LCMSn workflow
      -Accurate mass workflow
      -Search strategies
      -Compound coverage strategies

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    • Mass Spec - an Essential Tool for Characterization of mAbs during Development
      Mass Spec - an Essential Tool for Characterization of mAbs during Development Dr Wei Xu & Dr Henry Shion Recorded: Feb 3 2016 2:00 pm UTC 75 mins
    • Monoclonal antibodies (mAbs) represent a big portion of therapeutic proteins. Mass spectrometry (MS) coupled with modern separation technologies has become an essential tool in characterization of mAbs within the Quality by Design (QbD) paradigm during development. In this article, we use case studies to discuss the application of MS analysis in clone selection, optimization of fermentation conditions, development of purification and formulation. Specifically, simultaneously detect and monitor variants due to incomplete leader sequence processing, accurately determine afucosylation level of N-glycosylation, characterize host cell proteins (HCPs), identify degradation pathways and critical quality attributes (CQAs) will be discussed.

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    • Stable Isotope-Labeled Protein Internal Standards
      Stable Isotope-Labeled Protein Internal Standards James Walters, Ph.D. Processing: Sep 21 2017 7:15 pm UTC 60 mins
    • Mass spectrometry-based protein assays impart increased specificity and more rapid development times versus traditional methods, such as ELISA. Coupled with immunoaffinity enrichment, LC-MS/MS is becoming a powerful tool for the quantitation of proteins in plasma. Such methods typically rely on synthetic stable isotope labeled (SIL) peptide internal standards to correct for instrumental variability. For more accurate protein quantitation by LC-MS/MS, experimental variations throughout the entire sample preparation workflow, including protein fractionation, immunoaffinity enrichment, and enzymatic digestion, must be accounted for. An ideal way of improving assay reproducibility is to add a full-length stable isotope labeled recombinant protein, that is equivalent to the native target protein, to the sample at the initial stage of the assay workflow. We have developed a set of stable-isotope-labeled monoclonal antibodies expressed in CHO cells as well as SIL versions of several clinically-relevant human proteins expressed in E. coli, such as IGF1, and in mammalian HEK293 cells, such as Thyroglobulin (manufactured as a Certified Reference Material). We will present data to demonstrate that the use of full-length SIL proteins and antibodies as internal standards allows for more accurate and rapid quantitation of biotherapeutic antibodies and clinically-relevant human protein biomarkers in plasma by LC-MS/MS.

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    • Stable Isotope-Labeled Protein Internal Standards
      Stable Isotope-Labeled Protein Internal Standards James Walters, Ph.D. Recorded: Sep 20 2017 3:00 pm UTC 56 mins
    • Mass spectrometry-based protein assays impart increased specificity and more rapid development times versus traditional methods, such as ELISA. Coupled with immunoaffinity enrichment, LC-MS/MS is becoming a powerful tool for the quantitation of proteins in plasma. Such methods typically rely on synthetic stable isotope labeled (SIL) peptide internal standards to correct for instrumental variability. For more accurate protein quantitation by LC-MS/MS, experimental variations throughout the entire sample preparation workflow, including protein fractionation, immunoaffinity enrichment, and enzymatic digestion, must be accounted for. An ideal way of improving assay reproducibility is to add a full-length stable isotope labeled recombinant protein, that is equivalent to the native target protein, to the sample at the initial stage of the assay workflow. We have developed a set of stable-isotope-labeled monoclonal antibodies expressed in CHO cells as well as SIL versions of several clinically-relevant human proteins expressed in E. coli, such as IGF1, and in mammalian HEK293 cells, such as Thyroglobulin (manufactured as a Certified Reference Material). We will present data to demonstrate that the use of full-length SIL proteins and antibodies as internal standards allows for more accurate and rapid quantitation of biotherapeutic antibodies and clinically-relevant human protein biomarkers in plasma by LC-MS/MS.

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    • Particle Tracing Simulations with COMSOL Multiphysics®
      Particle Tracing Simulations with COMSOL Multiphysics® Dr. Edmund Dickinson Application Engineer COMSOL Recorded: Nov 11 2015 3:00 pm UTC 62 mins
    • The Particle Tracing Module extends the functionality of the COMSOL Multiphysics modelling environment, to allow the computation of the trajectories of particles in fluids and charged particles electromagnetic fields. The particles can be subjected to a wide variety of forces, such as electrical, drag, and thermophoretic forces, as well as particle-particle and fluid-particle interactions. This webinar introduces particle tracing for applications such as erosion, etching, mixing, filtration, mass spectrometry, ion optics, and beam physics. The webinar includes a demonstration and will end with a Q&A session.

      Edmund studied chemistry at the University of Oxford from 2004 to 2008. He then continued on for a doctorate at Oxford, researching the charge and mass transfer properties of electrolyte solutions. He joined COMSOL as an application engineer, where he specialises in chemistry, heat transfer and fluid flow.

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    • Enabling Reliable Compound Identification using LC-MSn
      Enabling Reliable Compound Identification using LC-MSn Herbert Oberacher Recorded: Nov 10 2015 3:00 pm UTC 53 mins
    • Enabling Reliable Compound Identification using LC-MSn

      Reliable compound identification with non-targeted liquid chromatography-tandem mass spectrometry (LC-MS/MS) is a major challenge. It involves matching against reference data. For fast and automated identification, tandem mass spectral libraries are queried with appropriate search tools. Reliability of search as well as time and efforts spent for data reviewing very much depend on the quality of the database involved. In this presentation, we will give an overview on our efforts towards the development of a reliable, robust, and transferable tandem mass spectral database, and how such a database can successfully be implemented in workflows for comprehensive drug analysis.

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    • From Screening to Confirmation: Integrated POPs Analysis Workflow
      From Screening to Confirmation: Integrated POPs Analysis Workflow Dirk Krumwiede, Hans-Joachim Huebschmann Recorded: Oct 6 2010 3:00 pm UTC 84 mins
    • This webinar focuses on the workflow for the analysis of Persistent Organic Pollutants (POPs) with the determination of dioxin/furans in food samples. A new concept for the combined and integrated use of screening and confirmation methods will be presented. The application of dedicated highly selective mass spectrometry technologies for the analysis of dioxins and furans in milk samples will be discussed using real-life data.

      What you will learn:

      - What is POPs screening? What is confirmation? How do the analytical requirements differ for these tasks?
      - Sample analysis workflow from fast screening to precise confirmation analysis.
      - Latest GC-MS/MS triple quadrupole mass spectrometry solution for optimum screening analysis.
      - Latest GC-HRMS magnetic sector mass spectrometry solution for optimum confirmation analysis.
      - Integrated data evaluation with a common software suite.
      - Approaches and investigations for an optimized sample preparation process.
      - Overall advantages of the integrated POPs analysis workflow.

      Who should attend:
      - Lab managers and Quality Assurance managers from food industry, contract and governmental laboratories.
      - Anybody interested in effective Dioxin/Furan analysis in food, feed environmental or biological samples.

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    • Molecular Annotation of EGFR Signaling-Associated Complexes in Human Cancer 02
      Molecular Annotation of EGFR Signaling-Associated Complexes in Human Cancer 02 Eric B. Haura M.D., Moffitt Cancer Center Recorded: Mar 26 2015 3:00 pm UTC 53 mins
    • Mass spectrometry and other methods such as yeast two-hybrid can now accurately discern protein complexes or larger protein interactomes in diseases such as cancer, yet it is difficult to forward translate this knowledge into human samples. To overcome this hurdle, we began experiments using proximity ligation assays (PLA) to directly translate protein complexes into human tumor materials. Our assay reflects protein complexes between EGFR and GRB2 protein, a key adaptor protein necessary for EGFR pathway activation and coupling to downstream MAPK signaling. We annotated nearly 300 primary xenograft models (PDX) of cancer and show tumor subtype enrichment of EGFR:GRB2 signaling-associated complexes. Furthermore, tumors with abundant levels of EGFR:GRB2 signaling-associated complexes are more likely to respond to anti-EGFR antibody-based therapy. Finally, in 350 lung cancer tissues, across three distinct cohorts of patients, we demonstrate the ability of EGFR:GRB2 protein complexes to segregate tumors and show benefit to EGFR tyrosine kinase inhibitor therapy in patients whose tumors harbor high levels of EGFR:GRB2 signaling-associated complexes. This suggests that annotation of signaling-associated protein complexes in cancer tissues can not only molecularly annotate disease types but may also have predictive capacity for cancer therapeutics. This work opens up the human protein interactome as a new class of molecular markers for disease in a more practical manner. Proteins, encoded by DNA, do not work in isolation but instead function as part of multi-protein complexes that drive both normal and disease physiology. While we demonstrate the utility of this approach in cancer, receptor tyrosine kinase signaling and tyrosine kinase inhibitor therapeutics, our approach described here could have utility across a wide spectrum of both signaling-associated complexes and different types of disease, and thus would be attractive to a large audience.

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    • Molecular Annotation of EGFR Signaling-Associated Complexes in Human Cancer 01
      Molecular Annotation of EGFR Signaling-Associated Complexes in Human Cancer 01 Eric B. Haura M.D., Moffitt Cancer Center Recorded: Mar 26 2015 1:00 am UTC 50 mins
    • Mass spectrometry and other methods such as yeast two-hybrid can now accurately discern protein complexes or larger protein interactomes in diseases such as cancer, yet it is difficult to forward translate this knowledge into human samples. To overcome this hurdle, we began experiments using proximity ligation assays (PLA) to directly translate protein complexes into human tumor materials. Our assay reflects protein complexes between EGFR and GRB2 protein, a key adaptor protein necessary for EGFR pathway activation and coupling to downstream MAPK signaling. We annotated nearly 300 primary xenograft models (PDX) of cancer and show tumor subtype enrichment of EGFR:GRB2 signaling-associated complexes. Furthermore, tumors with abundant levels of EGFR:GRB2 signaling-associated complexes are more likely to respond to anti-EGFR antibody-based therapy. Finally, in 350 lung cancer tissues, across three distinct cohorts of patients, we demonstrate the ability of EGFR:GRB2 protein complexes to segregate tumors and show benefit to EGFR tyrosine kinase inhibitor therapy in patients whose tumors harbor high levels of EGFR:GRB2 signaling-associated complexes. This suggests that annotation of signaling-associated protein complexes in cancer tissues can not only molecularly annotate disease types but may also have predictive capacity for cancer therapeutics. This work opens up the human protein interactome as a new class of molecular markers for disease in a more practical manner. Proteins, encoded by DNA, do not work in isolation but instead function as part of multi-protein complexes that drive both normal and disease physiology. While we demonstrate the utility of this approach in cancer, receptor tyrosine kinase signaling and tyrosine kinase inhibitor therapeutics, our approach described here could have utility across a wide spectrum of both signaling-associated complexes and different types of disease, and thus would be attractive to a large audience.

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    • The Science of Food Profiling
      The Science of Food Profiling Dr. Tim Jenkins Recorded: Nov 10 2009 4:00 pm UTC 55 mins
    • The Science of Food Profiling: Determining the Adulteration, Authenticity and Origin of Foods

      With reports of 5 to 10% of global food trade involving counterfeit goods, regulatory agencies and food manufacturers are compelled to employ programs that help ensure consumer safety, protect trade markets, maintain product quality and preserve brand image.

      This webinar will demonstrate how the latest analytical instrumentation and data analysis software can assist food testing laboratories in the determination of food adulteration, authenticity and origin.

      • Liquid chromatography and mass spectrometry are key analytical technologies for food profiling. The application and advantages of these techniques will be discussed.

      • Employing chemometrics software for automated data analyses can yield critical information for food testing laboratories. The approach facilitates the identification of differences or similarities between brands or manufacturing batches. This methodology will be examined.

      • Examples of edible oil, tea and fruit juice food profiling applications will be shown.

      Presenter

      Dr. Tim Jenkins is part of Waters Chemical Analysis Market Development group where he leads the Business Operations team. This group has particular expertise in food testing and environmental applications and addresses global market development issues. Prior to joining Waters Dr. Jenkins gained over 15 years experience of practical mass spectrometry and technical management in a variety of environments including academia, corporate R&D and contract analysis laboratories.

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    • Harnessing the Power of High-Res, Accurate Mass LC/MS Technology
      Harnessing the Power of High-Res, Accurate Mass LC/MS Technology Dipankar Ghosh, Ph.D., Tina Hemenway, Ph.D.,Thermo Fisher Scientific Recorded: Jan 25 2011 4:00 pm UTC 63 mins
    • Harnessing the Power of High-Resolution, Accurate Mass LC/MS Technology in Food Safety Laboratories

      In this webinar, you will learn the advantages of high-resolution, accurate mass LC/MS technology and how it can be applied in the food safety laboratory. Instrumentation that offers high resolving power and ultimate mass accuracy provide unique advantages in screening and quantifying low levels of contaminants in complex food matrices. Software is the key to efficient processing of this data to obtain quantitative and qualitative results. Attend this webinar to learn what features to look for in a high-resolution solution for your lab:

      -- What do you need to streamline your workflow and increase throughput?
      -- How are results verified so you have a high level of confidence in your results?
      -- How can you screen for unknowns?

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    • Overcoming Practical Challenges of Pesticide Residue Analysis
      Overcoming Practical Challenges of Pesticide Residue Analysis Ken Rosnack, Waters Corporation Recorded: Sep 14 2010 3:00 pm UTC 64 mins
    • Overcoming Practical Challenges of Pesticide Residue Analysis Through Integrated Technology and Workflow Solutions

      One of the biggest challenges in ensuring the safety of our food supplies is the measurement of hazardous, ultra trace level components in the presence of a highly complex sample matrix. For the analysis of pesticides in food matrices the increased use of liquid chromatography systems coupled with tandem quadrupole mass spectrometers has allowed progress in reducing the problems caused by the sample matrix. This talk will focus on an integrated workflow for multi-residue pesticide analysis in complex matrices. The ability to understand the matrix challenge of each injected sample is clearly beneficial, as is the ability to monitor changes in the sample matrix between samples and batches. Integrating an effective workflow can lead to the continuous improvement of productivity and analytical quality in the laboratory. Discussions will include method development / optimization by automating set up of the MS hardware (mass resolution, mass calibration, ion source optimization) as well as the process of developing compound-specific MRM data acquisition methods.

      Webinar Speaker:

      Ken Rosnack
      Business Development Manager
      World Wide Chemical Analysis Marketing Group
      Waters Corporation

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    • Retention Mechanisms in HILIC Chromatography:  Robust Method Development
      Retention Mechanisms in HILIC Chromatography: Robust Method Development David S. Bell, Ph.D. Recorded: Jun 28 2012 6:00 pm UTC 55 mins
    • Hydrophilic interaction liquid chromatography (HILIC), especially in conjunction with mass spectrometry (MS), has become a powerful tool for the analysis of a wide variety of challenging analytes. Applications of the technique have increased dramatically over the past decade, especially for the analysis of polar analytes where reversed-phase chromatography suffers. HILIC conditions employ a high percentage of acetonitrile which enables facilitated solvent evaporation in LC/MS sources and thus often an increase in analyte response when compared to more aqueous based systems. The increased retention of polar analytes afforded by HILIC provides improved selectivity and decreases the impact of endogenous species, often leading to improved qualitative and quantitative analyses.

      Although HILIC has proven useful, it has also been thwarted with complications including difficulties in method development and method robustness.

      In this presentation, studies investigating the underlying retention mechanisms dominant in HILIC chromatography are presented and discussed. Along with reversed-partitioning HILIC is well known to exhibit, ion-exchange and the interplay of the dominant mechanisms are unveiled and used to develop a model of overall retention and selectivity. Interactions that operate using different stationary phase chemistries and conditions are presented. The impact of analyte polarity and charge as well as the variations caused by high percentages of organic on these physiochemical parameters are highlighted. Throughout the discussion, examples of use and misuse of HILIC are employed to illustrate these important concepts to build a solid fundamental foundation for efficient and effective use of this powerful technique.

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    • Sports Drug Testing: Chromatography & Mass Spec-Based Approaches
      Sports Drug Testing: Chromatography & Mass Spec-Based Approaches Professor Mario Thevis Recorded: Sep 9 2010 3:00 pm UTC 51 mins
    • Doping control analysis predominantly utilises chromatography and mass spectrometry-based approaches to detect prohibited substances and methods of doping. These compounds and methods present both low and high molecular weight analytes of xenobiotic or natural / endogenous origin, which are to be detected, and occasionally quantified, using state-of-the-art instruments.

      The majority of the employed tools provides low resolving power. However, high resolution / high accuracy mass spectrometry has gained much attention recently due to: constantly increasing analytical requirements concerning the number of target compounds; the complexity of analytes (e.g. peptides and proteins); and the desire to accelerate analyses and obtain information (allowing for retrospective data mining).

      A selection of compounds, new challenges, and methods currently employed in doping control laboratories will be presented to the audience including, for example: new anabolic agents referred to as selective androgen receptor modulators (SARMs); insulins; so-called "releasing peptides" that stimulate the endogenous production of natural hormones; and ways of manipulating drug tests.

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    • Reducing Bottlenecks In GxP Laboratories
      Reducing Bottlenecks In GxP Laboratories Chris Stumpf, Ph.D. Recorded: Jan 19 2010 4:00 pm UTC 64 mins
    • Reducing Bottlenecks In GxP Laboratories With Electronic SOP Document And Workflow Systems

      In the QC laboratory, adherence to Good Manufacturing Practice (GMP) guidelines necessitates the need to maintain thorough documentation to ensure strict compliance with established procedures. Completing paper documents and ensuring their authenticity creates a burdensome bottleneck for the QC laboratory. This presentation will discuss strategies for using an electronic SOP document and workflow system to boost laboratory productivity.

      • Electronic SOP document and workflow systems improve SOP completion times, reduce procedural and transcription errors, and reduce review & sign-off times

      • A more efficient SOP documentation process translates into a more efficient documentation workflow, more accurate records, and faster product release.

      • An electronic SOP documentation system offers the opportunity to address the three major tenets of Lean Process manufacturing: eliminate waste, improve workflows, and improve quality.

      SPEAKER

      Chris Stumpf, Ph.D.
      Senior Product Marketing Manager, Informatics
      Waters Corporation

      Chris joined Waters in 2001 after earning his Ph.D. in Analytical Chemistry and Mass Spectrometry from Purdue University in 2000, and held a post doctoral position at the University of Cincinnati Medical School conducting proteomics research. During his doctorate and post-doctorate education, he became keenly interested in informatics solutions to manage and mine analytical data

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