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    • Accelerating Pharma R&D using Mass Spectrometry and NMR Accelerating Pharma R&D using Mass Spectrometry and NMR University of Dundee & Bruker Daltonics Recorded: May 26 2015 2:00 pm UTC 54 mins
    • Accelerating Pharma R&D with Novel MS Applications for High Throughput Screening (HTS), Drug Imaging and Integrating MS and NMR data for high confidence structure verification of small molecules.

      This webinar will introduce novel MS and NMR solutions and its applications in pharmaceutical research:

      > NMR Spectroscopy and Mass Spectrometry are two fundamental techniques for small molecule structure verification. Bruker FUSION-SV - a new software solution for comprehensive fully automated small molecule structure verification – simplifies and accelerates the drug design and discovery phases in the pharmaceutical industry significantly. The new Bruker FUSION-SV™ is a software solution integrating high resolution accurate mass (HRAM) data and complementary NMR data, into one result - increasing significantly the specificity and throughput for small organic molecule structure verification. Synthesis chemists will get a prompt result without being an expert spectroscopist.

      > Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) instruments are ideally suited for high throughput screening (HTS) in drug discovery. Not only speed, simplicity and robustness are the convincing arguments for MALDI-TOF MS but the inherent strength of a label- and solvent-free technique. The webinar will explain the technique and advantages of MALDI-TOF MS and present automation solutions for highest throughput and speed (less than 1 second per sample).

      > Finally the webinar will describe how the MALDI technology is used for small molecule imaging when coupled to modern 7 T (Tesla) FT-MS instrumentation. Drugs and metabolites can be visualized and distinguished one from the other directly measured from tissue samples. These results can be linked with histological information derived from the very same tissue, giving new insights into drug action and toxicity.

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    • Improve Your Mass Spectrometry Workflows With the New NIST Software Improve Your Mass Spectrometry Workflows With the New NIST Software Prof David Sparkman Recorded: Apr 23 2015 1:30 pm UTC 77 mins
    • Improve Your Mass Spectrometry Workflows With the New NIST Software, Featuring the Wiley 10th/NIST 2014

      Hosted by Prof David Sparkman

      Release 2.2 is one of the most significant improvements in NIST’s MS Search software. Prof Sparkman will lead an online webinar providing insight into the latest improvements in the most popular mass spectrometry program on the planet, with insights into how you can improve and speed up your analyses by using the full suite of functions available in MS Search 2.2 and the power of using these with the broadest compound coverage available.
      -Compatibility and systems interface tips
      -Retention time workflow
      -LCMSn workflow
      -Accurate mass workflow
      -Search strategies
      -Compound coverage strategies

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    • Mass Spec - an Essential Tool for Characterization of mAbs during Development Mass Spec - an Essential Tool for Characterization of mAbs during Development Dr Wei Xu & Dr Henry Shion Recorded: Feb 3 2016 2:00 pm UTC 75 mins
    • Monoclonal antibodies (mAbs) represent a big portion of therapeutic proteins. Mass spectrometry (MS) coupled with modern separation technologies has become an essential tool in characterization of mAbs within the Quality by Design (QbD) paradigm during development. In this article, we use case studies to discuss the application of MS analysis in clone selection, optimization of fermentation conditions, development of purification and formulation. Specifically, simultaneously detect and monitor variants due to incomplete leader sequence processing, accurately determine afucosylation level of N-glycosylation, characterize host cell proteins (HCPs), identify degradation pathways and critical quality attributes (CQAs) will be discussed.

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    • Increasing throughput and sensitivity of LC-MS assays Increasing throughput and sensitivity of LC-MS assays AB Sciex: Jason Causon, Senior Applications Specialist & Remco van Soest, Product Manager Recorded: Jul 28 2015 2:00 pm UTC 63 mins
    • Presentation 1: Jason Causon, Senior Applications Specialist, Sciex

      Development of bioanalytical LC-MS methods for the detection of therapeutic peptides and the proteolytic fragments of larger proteins and antibodies is growing along with the increasing number of peptide and protein drugs entering clinical research and development. Development of these methods present challenges that are different to small molecule method development because of the multiple charge states possible for a given peptide, the abundance of product ion possibilities and the large number of proteolytic fragments possible from a protein digest. In addition to optimizing for sensitivity, on-column HPLC method development is time consuming for the same reasons. In this webinar we present an automated tuning and optimization workflow with on-column validation using DiscoveryQuantTM 3.0 software with the new ChromaTune feature to overcome some of these challenges and make the optimization of peptide MRM methods easier and less time consuming.

      Presentation 2: Remco van Soest, Product Manager, Sciex

      Electrospray Ionization efficiency increases at lower flow rates. In this Webinar we will discuss how microLC can help you improve sensitivity in sample limited applications in bio-analysis by LC-MS. In order to analyse the same volume of sample as is typically injected in a conventional LC-MS system, up to 50 µL, we have developed a microLC system that allows for fast on-line pre-concentration on a short trap column. Because of the higher flow rates that can be used for loading the sample on the trap-column, sample throughput is not affected by using microLC flow rates for the LC-MS analysis.

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    • The detection and screening of mycotoxins by LC-MS/MS The detection and screening of mycotoxins by LC-MS/MS Dr Stephen Lock PhD, Dr Ondrej Lacina PhD, Dr Florian Huebner Recorded: Sep 20 2012 8:30 am UTC 63 mins
    • This webinar covers the different approaches to the detection of mycotoxin contamination in food by LC-MS/MS. Examples are covered using highly sensitive systems to simplify the extraction and analysis and detection of these toxins at low levels. The additional benefits of using accurate mass or SelexION™ ion mobility in this application area are also covered.

      Presentations:

      * Screening of mycotoxins in Food by triple quadrupole and qtrap LC-MS/MS - Dr Stephen Lock PhD

      * High Resolution Tandem Mass Spectrometry in mycotoxin analysis: higher selectivity to improve analyte confirmation - Dr Ondrej Lacina PhD

      * Boosting the performance of LC-MS/MS analysis of mycotoxins and their transformation products by using innovative separation techniques - Dr. Florian Huebner

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    • Particle Tracing Simulations with COMSOL Multiphysics® Particle Tracing Simulations with COMSOL Multiphysics® Dr. Edmund Dickinson Application Engineer COMSOL Recorded: Nov 11 2015 3:00 pm UTC 62 mins
    • The Particle Tracing Module extends the functionality of the COMSOL Multiphysics modelling environment, to allow the computation of the trajectories of particles in fluids and charged particles electromagnetic fields. The particles can be subjected to a wide variety of forces, such as electrical, drag, and thermophoretic forces, as well as particle-particle and fluid-particle interactions. This webinar introduces particle tracing for applications such as erosion, etching, mixing, filtration, mass spectrometry, ion optics, and beam physics. The webinar includes a demonstration and will end with a Q&A session.

      Edmund studied chemistry at the University of Oxford from 2004 to 2008. He then continued on for a doctorate at Oxford, researching the charge and mass transfer properties of electrolyte solutions. He joined COMSOL as an application engineer, where he specialises in chemistry, heat transfer and fluid flow.

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    • Enabling Reliable Compound Identification using LC-MSn Enabling Reliable Compound Identification using LC-MSn Herbert Oberacher Recorded: Nov 10 2015 3:00 pm UTC 53 mins
    • Enabling Reliable Compound Identification using LC-MSn

      Reliable compound identification with non-targeted liquid chromatography-tandem mass spectrometry (LC-MS/MS) is a major challenge. It involves matching against reference data. For fast and automated identification, tandem mass spectral libraries are queried with appropriate search tools. Reliability of search as well as time and efforts spent for data reviewing very much depend on the quality of the database involved. In this presentation, we will give an overview on our efforts towards the development of a reliable, robust, and transferable tandem mass spectral database, and how such a database can successfully be implemented in workflows for comprehensive drug analysis.

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    • From Screening to Confirmation: Integrated POPs Analysis Workflow From Screening to Confirmation: Integrated POPs Analysis Workflow Dirk Krumwiede, Hans-Joachim Huebschmann Recorded: Oct 6 2010 3:00 pm UTC 84 mins
    • This webinar focuses on the workflow for the analysis of Persistent Organic Pollutants (POPs) with the determination of dioxin/furans in food samples. A new concept for the combined and integrated use of screening and confirmation methods will be presented. The application of dedicated highly selective mass spectrometry technologies for the analysis of dioxins and furans in milk samples will be discussed using real-life data.

      What you will learn:

      - What is POPs screening? What is confirmation? How do the analytical requirements differ for these tasks?
      - Sample analysis workflow from fast screening to precise confirmation analysis.
      - Latest GC-MS/MS triple quadrupole mass spectrometry solution for optimum screening analysis.
      - Latest GC-HRMS magnetic sector mass spectrometry solution for optimum confirmation analysis.
      - Integrated data evaluation with a common software suite.
      - Approaches and investigations for an optimized sample preparation process.
      - Overall advantages of the integrated POPs analysis workflow.

      Who should attend:
      - Lab managers and Quality Assurance managers from food industry, contract and governmental laboratories.
      - Anybody interested in effective Dioxin/Furan analysis in food, feed environmental or biological samples.

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    • Novel Techniques and New Advances in the Analyses of Fat species in Foods Novel Techniques and New Advances in the Analyses of Fat species in Foods Len Sidisky from Sigma-Aldrich & Dr Peter Q. Tranchida from University of Messina Recorded: Nov 19 2015 3:00 pm UTC 48 mins
    • Th​is webinar will provide an overview on the topic of analyzing various fatty species in foods and methods to determine various classes from total fat to various lipid fractions and the wide variety of analyses available for fat determination. Topics discussed will include the analyses of fatty acid methyl esters (FAMES) in foods and the use of comprehensive two-dimensional gas chromatography (GC x GC) for the enhanced separation of highly complex mixtures. Advances in GC column stationary phases and new phases based on ionic liquid technology for the analyses of saturated and polyunsaturated FAMES will be discussed aswell as the use of GC×GC in the field of food lipid analysis. The considerable advantages of the two-dimensional approach (both with and without MS detection), in experiments related to a series of lipid volatiles (fatty acids, sterols, oxidation products, fatty alcohols, etc) will be illustrated.

      In this webinar, we will:

      > Overview the wide variety of methods available for the analysis of fat in foods.

      > Examine traditional and new classes of GC Stationary Phases for the evaluation of various FAME species.

      > Discuss the power of two-dimensional gas chromatography (GCxGC) for separating highly complex mixtures and how it can be combined with mass spectrometry for analyzing volatile and semi-volatile analytes

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    • Molecular Annotation of EGFR Signaling-Associated Complexes in Human Cancer 02 Molecular Annotation of EGFR Signaling-Associated Complexes in Human Cancer 02 Eric B. Haura M.D., Moffitt Cancer Center Recorded: Mar 26 2015 3:00 pm UTC 53 mins
    • Mass spectrometry and other methods such as yeast two-hybrid can now accurately discern protein complexes or larger protein interactomes in diseases such as cancer, yet it is difficult to forward translate this knowledge into human samples. To overcome this hurdle, we began experiments using proximity ligation assays (PLA) to directly translate protein complexes into human tumor materials. Our assay reflects protein complexes between EGFR and GRB2 protein, a key adaptor protein necessary for EGFR pathway activation and coupling to downstream MAPK signaling. We annotated nearly 300 primary xenograft models (PDX) of cancer and show tumor subtype enrichment of EGFR:GRB2 signaling-associated complexes. Furthermore, tumors with abundant levels of EGFR:GRB2 signaling-associated complexes are more likely to respond to anti-EGFR antibody-based therapy. Finally, in 350 lung cancer tissues, across three distinct cohorts of patients, we demonstrate the ability of EGFR:GRB2 protein complexes to segregate tumors and show benefit to EGFR tyrosine kinase inhibitor therapy in patients whose tumors harbor high levels of EGFR:GRB2 signaling-associated complexes. This suggests that annotation of signaling-associated protein complexes in cancer tissues can not only molecularly annotate disease types but may also have predictive capacity for cancer therapeutics. This work opens up the human protein interactome as a new class of molecular markers for disease in a more practical manner. Proteins, encoded by DNA, do not work in isolation but instead function as part of multi-protein complexes that drive both normal and disease physiology. While we demonstrate the utility of this approach in cancer, receptor tyrosine kinase signaling and tyrosine kinase inhibitor therapeutics, our approach described here could have utility across a wide spectrum of both signaling-associated complexes and different types of disease, and thus would be attractive to a large audience.

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    • Molecular Annotation of EGFR Signaling-Associated Complexes in Human Cancer 01 Molecular Annotation of EGFR Signaling-Associated Complexes in Human Cancer 01 Eric B. Haura M.D., Moffitt Cancer Center Recorded: Mar 26 2015 1:00 am UTC 50 mins
    • Mass spectrometry and other methods such as yeast two-hybrid can now accurately discern protein complexes or larger protein interactomes in diseases such as cancer, yet it is difficult to forward translate this knowledge into human samples. To overcome this hurdle, we began experiments using proximity ligation assays (PLA) to directly translate protein complexes into human tumor materials. Our assay reflects protein complexes between EGFR and GRB2 protein, a key adaptor protein necessary for EGFR pathway activation and coupling to downstream MAPK signaling. We annotated nearly 300 primary xenograft models (PDX) of cancer and show tumor subtype enrichment of EGFR:GRB2 signaling-associated complexes. Furthermore, tumors with abundant levels of EGFR:GRB2 signaling-associated complexes are more likely to respond to anti-EGFR antibody-based therapy. Finally, in 350 lung cancer tissues, across three distinct cohorts of patients, we demonstrate the ability of EGFR:GRB2 protein complexes to segregate tumors and show benefit to EGFR tyrosine kinase inhibitor therapy in patients whose tumors harbor high levels of EGFR:GRB2 signaling-associated complexes. This suggests that annotation of signaling-associated protein complexes in cancer tissues can not only molecularly annotate disease types but may also have predictive capacity for cancer therapeutics. This work opens up the human protein interactome as a new class of molecular markers for disease in a more practical manner. Proteins, encoded by DNA, do not work in isolation but instead function as part of multi-protein complexes that drive both normal and disease physiology. While we demonstrate the utility of this approach in cancer, receptor tyrosine kinase signaling and tyrosine kinase inhibitor therapeutics, our approach described here could have utility across a wide spectrum of both signaling-associated complexes and different types of disease, and thus would be attractive to a large audience.

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    • The Science of Food Profiling The Science of Food Profiling Dr. Tim Jenkins Recorded: Nov 10 2009 4:00 pm UTC 55 mins
    • The Science of Food Profiling: Determining the Adulteration, Authenticity and Origin of Foods

      With reports of 5 to 10% of global food trade involving counterfeit goods, regulatory agencies and food manufacturers are compelled to employ programs that help ensure consumer safety, protect trade markets, maintain product quality and preserve brand image.

      This webinar will demonstrate how the latest analytical instrumentation and data analysis software can assist food testing laboratories in the determination of food adulteration, authenticity and origin.

      • Liquid chromatography and mass spectrometry are key analytical technologies for food profiling. The application and advantages of these techniques will be discussed.

      • Employing chemometrics software for automated data analyses can yield critical information for food testing laboratories. The approach facilitates the identification of differences or similarities between brands or manufacturing batches. This methodology will be examined.

      • Examples of edible oil, tea and fruit juice food profiling applications will be shown.

      Presenter

      Dr. Tim Jenkins is part of Waters Chemical Analysis Market Development group where he leads the Business Operations team. This group has particular expertise in food testing and environmental applications and addresses global market development issues. Prior to joining Waters Dr. Jenkins gained over 15 years experience of practical mass spectrometry and technical management in a variety of environments including academia, corporate R&D and contract analysis laboratories.

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    • Harnessing the Power of High-Res, Accurate Mass LC/MS Technology Harnessing the Power of High-Res, Accurate Mass LC/MS Technology Dipankar Ghosh, Ph.D., Tina Hemenway, Ph.D.,Thermo Fisher Scientific Recorded: Jan 25 2011 4:00 pm UTC 63 mins
    • Harnessing the Power of High-Resolution, Accurate Mass LC/MS Technology in Food Safety Laboratories

      In this webinar, you will learn the advantages of high-resolution, accurate mass LC/MS technology and how it can be applied in the food safety laboratory. Instrumentation that offers high resolving power and ultimate mass accuracy provide unique advantages in screening and quantifying low levels of contaminants in complex food matrices. Software is the key to efficient processing of this data to obtain quantitative and qualitative results. Attend this webinar to learn what features to look for in a high-resolution solution for your lab:

      -- What do you need to streamline your workflow and increase throughput?
      -- How are results verified so you have a high level of confidence in your results?
      -- How can you screen for unknowns?

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    • Overcoming Practical Challenges of Pesticide Residue Analysis Overcoming Practical Challenges of Pesticide Residue Analysis Ken Rosnack, Waters Corporation Recorded: Sep 14 2010 3:00 pm UTC 64 mins
    • Overcoming Practical Challenges of Pesticide Residue Analysis Through Integrated Technology and Workflow Solutions

      One of the biggest challenges in ensuring the safety of our food supplies is the measurement of hazardous, ultra trace level components in the presence of a highly complex sample matrix. For the analysis of pesticides in food matrices the increased use of liquid chromatography systems coupled with tandem quadrupole mass spectrometers has allowed progress in reducing the problems caused by the sample matrix. This talk will focus on an integrated workflow for multi-residue pesticide analysis in complex matrices. The ability to understand the matrix challenge of each injected sample is clearly beneficial, as is the ability to monitor changes in the sample matrix between samples and batches. Integrating an effective workflow can lead to the continuous improvement of productivity and analytical quality in the laboratory. Discussions will include method development / optimization by automating set up of the MS hardware (mass resolution, mass calibration, ion source optimization) as well as the process of developing compound-specific MRM data acquisition methods.

      Webinar Speaker:

      Ken Rosnack
      Business Development Manager
      World Wide Chemical Analysis Marketing Group
      Waters Corporation

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    • Retention Mechanisms in HILIC Chromatography:  Robust Method Development Retention Mechanisms in HILIC Chromatography: Robust Method Development David S. Bell, Ph.D. Recorded: Jun 28 2012 6:00 pm UTC 55 mins
    • Hydrophilic interaction liquid chromatography (HILIC), especially in conjunction with mass spectrometry (MS), has become a powerful tool for the analysis of a wide variety of challenging analytes. Applications of the technique have increased dramatically over the past decade, especially for the analysis of polar analytes where reversed-phase chromatography suffers. HILIC conditions employ a high percentage of acetonitrile which enables facilitated solvent evaporation in LC/MS sources and thus often an increase in analyte response when compared to more aqueous based systems. The increased retention of polar analytes afforded by HILIC provides improved selectivity and decreases the impact of endogenous species, often leading to improved qualitative and quantitative analyses.

      Although HILIC has proven useful, it has also been thwarted with complications including difficulties in method development and method robustness.

      In this presentation, studies investigating the underlying retention mechanisms dominant in HILIC chromatography are presented and discussed. Along with reversed-partitioning HILIC is well known to exhibit, ion-exchange and the interplay of the dominant mechanisms are unveiled and used to develop a model of overall retention and selectivity. Interactions that operate using different stationary phase chemistries and conditions are presented. The impact of analyte polarity and charge as well as the variations caused by high percentages of organic on these physiochemical parameters are highlighted. Throughout the discussion, examples of use and misuse of HILIC are employed to illustrate these important concepts to build a solid fundamental foundation for efficient and effective use of this powerful technique.

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    • Sports Drug Testing: Chromatography & Mass Spec-Based Approaches Sports Drug Testing: Chromatography & Mass Spec-Based Approaches Professor Mario Thevis Recorded: Sep 9 2010 3:00 pm UTC 51 mins
    • Doping control analysis predominantly utilises chromatography and mass spectrometry-based approaches to detect prohibited substances and methods of doping. These compounds and methods present both low and high molecular weight analytes of xenobiotic or natural / endogenous origin, which are to be detected, and occasionally quantified, using state-of-the-art instruments.

      The majority of the employed tools provides low resolving power. However, high resolution / high accuracy mass spectrometry has gained much attention recently due to: constantly increasing analytical requirements concerning the number of target compounds; the complexity of analytes (e.g. peptides and proteins); and the desire to accelerate analyses and obtain information (allowing for retrospective data mining).

      A selection of compounds, new challenges, and methods currently employed in doping control laboratories will be presented to the audience including, for example: new anabolic agents referred to as selective androgen receptor modulators (SARMs); insulins; so-called "releasing peptides" that stimulate the endogenous production of natural hormones; and ways of manipulating drug tests.

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    • Reducing Bottlenecks In GxP Laboratories Reducing Bottlenecks In GxP Laboratories Chris Stumpf, Ph.D. Recorded: Jan 19 2010 4:00 pm UTC 64 mins
    • Reducing Bottlenecks In GxP Laboratories With Electronic SOP Document And Workflow Systems

      In the QC laboratory, adherence to Good Manufacturing Practice (GMP) guidelines necessitates the need to maintain thorough documentation to ensure strict compliance with established procedures. Completing paper documents and ensuring their authenticity creates a burdensome bottleneck for the QC laboratory. This presentation will discuss strategies for using an electronic SOP document and workflow system to boost laboratory productivity.

      • Electronic SOP document and workflow systems improve SOP completion times, reduce procedural and transcription errors, and reduce review & sign-off times

      • A more efficient SOP documentation process translates into a more efficient documentation workflow, more accurate records, and faster product release.

      • An electronic SOP documentation system offers the opportunity to address the three major tenets of Lean Process manufacturing: eliminate waste, improve workflows, and improve quality.

      SPEAKER

      Chris Stumpf, Ph.D.
      Senior Product Marketing Manager, Informatics
      Waters Corporation

      Chris joined Waters in 2001 after earning his Ph.D. in Analytical Chemistry and Mass Spectrometry from Purdue University in 2000, and held a post doctoral position at the University of Cincinnati Medical School conducting proteomics research. During his doctorate and post-doctorate education, he became keenly interested in informatics solutions to manage and mine analytical data

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