High-Resolution Optical and Dielectric Methods Monitoring Cells in Bioprocesses

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Presented by

Michael Butler, PhD, Professor and Principal Investigator, National Institute for Bioprocessing Research and Training (NIBRT)

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Presented by Michael Butler, PhD, Professor and Principal Investigator, National Institute for Bioprocessing Research and Training (NIBRT) The metabolic state of mammalian cells in a bioprocess can be monitored by high-resolution optical systems or by changes in dielectric properties. Each method can determine a metabolic inflection points just prior to loss of cell viability. Such changes can be observed up to 20h before cells stain with trypan blue. We have established a threshold of cytoplasmic conductivity below which cells cannot be recovered from apoptosis. This point occurs at a time when there are observable changes in the cell membrane that can be detected by high-resolution optics. This early detection of metabolic changes allows dielectrics and high-resolution optics to be offered as a means of monitoring a bioprocess and maintaining cell viability over prolonged time periods.

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