Practical Utilization of CRISPR in the Laboratory Workflow - Session 2

Presented by

Erika Holroyd, ZFN and CRISPR Technical Scientist

About this talk

This 40 minute webinar will focus on the practical laboratory techniques needed to create and validate genetically modified cell lines using CRISPR. A Sigma-Aldrich® technology expert will discuss guidelines and techniques utilized when performing genome editing experiments. Topics will include: •Transfection of CRISPR reagent into a cell line •How to validate the activity level of CRISPR •Clonal isolation •How to screen clonal populations based on desired modification

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