Name: Gene Synthesis as an Alternative to Molecular Cloning Session 2
Start: 2016-02-16T14:00:00Z
End: 2016-02-16T14:30:22.000Z
Location: BrightTALK
Rating: 3

The Life Science Business of Merck KGaA, Darmstadt, Germany Webinar Channel features scientific presentations from key specialists in analytical chemistry, biology, chemistry and life sciences on the practical and technical aspects of new developments and innovations, to help advance your research.

Imaging of live cells and tissues is a rapidly growing field and has revolutionized how biologists study cells, proteins and a multitude of processes and molecular interactions. Live cell imaging techniques allow scientists to observe cell structures, study dynamic processes and the localization of molecules in real time, and over time. The observation of dynamic changes provides more insight into the operations of a cell than a snapshot provided by imaging fixed cells. Moreover, the spatial resolution of light microscopy allows observing subcellular structures and cellular processes, by using a range of fluorescent probes. Hence, live cell probes are being used more frequently used, instead of antibodies. Moreover, since live cell imaging is less prone to experimental artifacts, it usually provides more reliable and relevant information than fixed cell microscopy.

In this webinar researchers will:
      • Learn about some of the new live cell probes for cell structures, reactive ions, endocytosis, 
         exocytosis, hypoxia, nucleotide transporters, and more.
      • Learn about probes for cellular stress by tracking reactive oxygen and nitrogen species in 
         real-time.  
      •Learn the benefits of imaging live cells as compared to fixed cells

Live Cell Imaging: Novel Probes and Imaging Reagents

Important updates to the IFU Method No. 12 for Alicyclobacillus. Spore-forming Thermo-acidophilic spoilage bacteria are one of the major frequent concerns in the juice and juice added beverages.

Update of the IFU Method No.12 for Alicyclobacillus spp

LC/MS is an excellent tool for analyzing samples. This presentation provides valuable tips and tricks to get the best performance out of your LC/MS instrument regarding sensitivity and limit the instrument downtime by different approaches. In detail HPLC column technologies as well as advanced LC/MS solvents and additives for improved LC/MS performance will be discussed. Furthermore, the use of reference materials as internal standards and for calibration will be addressed. Finally the importance of Sample Preparation for robust and consistent results will be highlighted.

Tips and Tricks for LC/MS

Have you ever found yourself wondering which clone of Napsin A to use? What about PAX-8? Or the ongoing dilemma of ordering a mouse monoclonal vs rabbit polyclonal? Then this webinar is for you! 
In this presentation we will explore how antibody's are produced in their corresponding hosts. From there we will identify the main differences between the most popular types of antibodies, including mouse and rabbit monoclonals, and rabbit polyclonals. We will then compare the performance of specific clones that are backed by NordiQC data. We hope this will aid in choosing the best clone for your IHC tests.

Clone Wars: An In Depth Look at Mouse vs Rabbit Antibodies

Il s’agit d’un webinar visant la stratégie de maîtrise de la qualité des dispositifs médicaux stériles avec un renforcement de leur réglementation et l’intérêt croissant de la surveillance environnementale de leurs zones à risque de fabrication donc à atmosphère maitrisée 
Rappel des bases de la surveillance de la biocontamination environnementale -Air Surface Personnel - des zones à atmosphère contrôlée des DM et informations sur le nouveau projet de norme européen EN 17141 sur le contrôle de la biocontamination des salles propres en préparation avec une annexe dédiée.

Importance de la surveillance microbiologique de l'environnement de fabrication

Interest in chromatography using hydrophilic interaction liquid chromatography (HILIC) has continued to build in recent years. Although HILIC chromatography is known to provide valuable retention and selectivity of polar compounds and provide highly compatible conditions for coupling with mass spectrometry, it is sometimes avoided due to issues surrounding robustness and repeatability. In this webinar stationary and mobile phase selection will be discussed in detail together with the impact of sample solvent and equilibration procedures on retention times. Based on this HILIC method development practices can be improve and better understanding of HILIC is gained.

Tutorial for a better understanding of HILIC

Fatty acids are important analytes for nutritional value, product taste/texture, shelf-life and place of origin studies. They are not only relevant in food, but also in clinical, biochemical, industrial, agriculture testing. 

In this talk we will give the background of fatty acid analysis and describe some current techniques and new developments for the GC analysis of fatty acids in a variety of applications. A special focus of the webinar is the separation of cis and trans isomers by either a sample prep approach using SPE or by improved GC approaches.

Superior Sample Preparation and GC of Fatty Acid Methyl Esters

It’s no secret that we live in a celebrity centric culture hungry for the latest news about our favorite famous faces. This lecture will employ the fascinating world of celebrities to introduce the immunohistochemical grid approach to cancer disease states. We will specifically use cancer stories from past and present entertainers, athletes, and politicians to examine the histological characteristics and statistics that define various tumor types. These cases will also be our launching pad as we take a deep dive into the intricate world of tumor immunophenotyping by highlighting recent antibody additions and their impact on modern detection. Attendees will learn the utility of these new antibodies and gain a better overall understating of how diagnostic grids are used by pathologists to provide thorough and accurate patient care.

Famous Cancers

Aplicaciones para agricultura, acuacultura y agua de mar a través del Método fotométrico.

Determinación de macro y micronutrientes

Evolutions des référentiels qualité et crises sanitaires, comment le nettoyage et la désinfection apportent des réponses aux changements majeurs. 
Nous présenterons les solutions de contrôle rapide du nettoyage allant des méthodes conventionnelles microbiologiques aux méthodes rapides en passant par le contrôle de l’air.

BRC v8 et ISO 22000 : 2018, du Système de management de la qualité au process de

Biomagnetic separation has proven to be a quick, efficient and clean process in Life Sciences. However, most researchers and developers focus only on the magnetic beads or particles to optimize their separation process. The effectivity of the biomagnetic separation depending on the magnetic carrier is only half of the story. To have the complete picture we also need to pay attention to the role of the applied magnetic field on the play. Not understanding or controlling the parameters linked to the magnetic separator will result in failure when developing new applications, and also in industrializing lab-scale developments. The webinar will review the basic concepts of magnetic separation and help the attendees understand how advanced systems may enlight key aspects of the process. These concepts will be applied to parameterize, monitor and validate the magnetic beads behavior in controlled conditions. Afterwards, the discussion will focus on how to transfer the correctly characterized biomagnetic separation process from laboratory to production scale. Finally, the webinar will address how to use this knowledge to assure the quality of the magnetic-carriers based products.

Enlighten Biomagnetic Separation Processes

Chemiluminescent immunoassays (CLIAs) offer one of the best solutions for the quantification of low concentrations of specific analytes from a complex mixture for in vitro diagnostic industry. The assay format is similar to enzyme-linked immunoassays, usually based on heterogeneous assays where antibodies or antigens are immobilized on a solid phase but one of the components is conjugated with a chemiluminescent label. 

 

The benefits of CLIAs can be enhanced using magnetic beads as a solid platform which improves the separation of the un-bound reagents and reduces the interferences using a magnetic field.  

 

The CLIA assays based on magnetic beads together with chemiluminescent tagging of immunoreagents are widely used in high throughput automated platforms obtaining an amplified signal and decreasing the matrix interferences. The complexity of these type of immunoassays rely on the optimization of several components and parameters. The critical points are highly related to the type of immunoassay format that best suits the desired specifications, magnetic beads selection and conjugation conditions for magnetic particles and chemiluminescent labelling parameters.

Development of Chemiluminescent Immunoassays Based on Magnetic Beads

Routine and special stains are usually selected to demonstrate a special structure, chemical or molecular characteristic of the tissue. They are the first tool in a pathologist’s arsenal in detecting cancer in a patient's tissue. If there still are questions, a pathologist will request more advanced staining. This workshop will look at the more commonly requested special stains, and we will review the methods, application and results of these stains. We will also look at the complimentary IHC stains and how they compare.

Routine, Special, and Advanced Staining for the IHC Laboratory

Solid Phase Microextraction (SPME) is an invaluable tool for sample preparation in Environmental Analysis, because it is a trace analysis technology that is easy to fully automate. SPME is method of choice in many official analytical methods In Environmental Testing. This webinar provides an introduction into SPME and showcases how SPME facilitates different environmental applications for water testing.

Improving Environmental Analysis using SPME in Sample Preparation

Advances in gene editing technologies have generated a great amount of interest within the scientific community over the past few years.  In addition to the ability to make precise double stranded DNA cuts virtually anywhere in the human genome, new variations of these tools show promise in the ability to activate or repress the expression of individual genes. Besides the obvious interest in clinical applications for these tools, there are practical uses of these tools for modifying and improving in vitro cell-based assays in areas such as preclinical ADME/Tox.  This webinar will highlight these recent advances in gene editing technology and provide several examples of how this technology has been applied to ADME/Tox assays, including intestinal, hepatic and renal proximal tubule cell lines.

Tapping the Potential of Gene Editing Technology to Improve Cell-Based Assays fo

Adoptive transfer of lymphocytes expressing engineered T cell receptors (TCRs) is a promising immunotherapeutic option which specifically targets antigens from viral-infected cells and tumors. Prof. Bertoletti and his team engineered a library of TCRs specific for different Hepatitis B Virus (HBV) antigens to generate a pool of HBV-specific TCR-T cells. These TCR-T cells have been shown to recognize HBV epitopes presented on infected hepatocytes and hepatocellular carcinoma (HCC) cells with HBV-DNA integration in both experimental models and selected patients with HBV-related HCC relapses. However, safety concerns on possible irreversible structural and functional liver damage by cytotoxic TCR-T cells have limited the clinical usage of this immunotherapeutic approach. To overcome this limitation, they selected patients with HCC relapses after liver transplantation for immunotherapy since TCR-T cells only target HBV epitopes presented on HCC-relapses. Moreover, they also modified TCR-T cell production methods to reduce cytotoxicity and life span of TCR-T cell. These strategies that lead to the successful production of safe TCR-T cell effectors for immunotherapy of HBV infection and HBV-related cancers will be discussed in this webinar.

Personalized T-cell therapy against HBV infection & HBV-related HCC

Latex enhanced immunoassay reagents can be adapted to automated biochemistry bio-analyzers ,equipment available in every modern biochemistry laboratory. Combining existing knowledge in the areas of antibody development and bead conjugation these reagents are suitable for the determination of proteins in patient samples. Their use can override the need for specific and expensive equipment necessary to perform these tests in the past. This talk will focus on the methods we could follow in the design of these reagents, the optimization of crucial factors in the development phase and the validation procedures to reach the expected analytical performance.

Principles for the Development of Latex Enhanced Immunoassays 

Gene synthesis has become a quick and cost-effective way to build your DNA construct for a variety of applications - from protein engineering to bio-based circuit and pathway design. This webinar, presented by Sigma-Aldrich and GENEWIZ will explain the process of gene synthesis, how it compares to traditional cloning techniques, and review some key applications researchers are addressing with synthetic DNA.

Gene Synthesis as an Alternative to Molecular Cloning Session 2

gene synthesis

synthetic DNA

protein

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Gene Synthesis as an Alternative to Molecular Cloning Session 2

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