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Designing the Next Phase in Genome Editing: Advanced CRISPR Applications

CRISPR Cas9 nucleases have revolutionized the field of genome editing enabling unprecedented efficiency of gene targeting in a vast array of cell types and organisms. Even with such powerful technology at hand, researchers who are new to the field may find genome modification to be challenging and time-consuming. As CRISPR becomes a focus of the molecular biology research community, MilliporeSigma seeks to share the best approaches learned and methods applied in our years of genome editing experience. Today’s presentation will focus on practical applications of CRISPR for pristine genome editing to achieve knockout as well as specific sequence changes to include donor-mediated snps, reporter-tags and conditional knockouts. Special attention will be paid to design considerations for the donor constructs necessary to achieve specific sequence changes. Finally, the frontiers of CRISPR technology, including synthetic crRNA to fast-track genome editing experiments, whole genome screening and targeted gene activation will be explored.
Recorded Mar 1 2016 44 mins
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Presented by
Jeremy Lehmann- CRISPR Product Specialist, Functional Genomics, MilliporeSigma
Presentation preview: Designing the Next Phase in Genome Editing: Advanced CRISPR Applications

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  • Title: Designing the Next Phase in Genome Editing: Advanced CRISPR Applications
  • Live at: Mar 1 2016 6:00 pm
  • Presented by: Jeremy Lehmann- CRISPR Product Specialist, Functional Genomics, MilliporeSigma
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