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A Practical 2D-LC System Developed for Complex Pharmaceuticals

A comprehensive two-dimensional liquid chromatograph (LC × LC) was constructed from commercially available conventional HPLC equipments. This system utilizes two independently configurable 2nd dimension binary pumping systems to deliver independent flow rates, gradient profiles and mobile phase compositions to dual Fused-Core secondary columns. Very fast gradient separations (30 seconds total cycle time) were achieved at ambient temperature without excessive backpressure and without compromising optimal 1st dimension sampling rates by using superficially porous stationary phases. A practical approach to optimize the various inter-related instrumental parameters will also be presented.
Recorded Mar 23 2011 31 mins
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Presented by
Lianjia Ma, Ph.D.* and Anthony Alexander, Ph.D.
Presentation preview: A Practical 2D-LC System Developed for Complex Pharmaceuticals

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  • Title: A Practical 2D-LC System Developed for Complex Pharmaceuticals
  • Live at: Mar 23 2011 3:00 pm
  • Presented by: Lianjia Ma, Ph.D.* and Anthony Alexander, Ph.D.
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