High Precision PCR utilizing ThermaGenix Reagents

Presented by

Professor Lawrence Wangh, Founder and CSO of ThermaGenix, Inc.

About this talk

The polymerase chain reaction (PCR) is a mainstay of molecular biology and genomics that provides an efficient and rapid in vitro method for enzymatic amplification of DNA or RNA sequences from various sources. There are three unique, easy-to-use PCR additives that act at different temperatures to improve sensitivity and product yield by preventing mis-priming: 1. ThermaStop™: a novel reagent that acts like a universal hot-start for Type A and Type B DNA polymerases 2. ThermaGo™: a unique reagent that enhances the specificity of these same enzymes during the course of amplification 3. ThermaStop™-RT: a first-in-class reagent that acts like a hot-start for many reverse transcriptases. Each reagent is a chemically modified oligonucleotide produced under GMP conditions and sold as a dry powder. Stable at room temperature, each reagent can simply be added to the enzyme of your choice prior to adding that enzyme/reagent complex to the master mix. These improvements are observed for both inexpensive Type A Taq polymerases and very expensive HiFi Type B DNA polymerases in applications such as qPCR, multiplexing, and preparation of DNA amplicons prior to next-generation sequencing (NGS).

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