Live Cell Imaging: Novel Probes and Imaging Reagents

Presented by

Dr. Mahesh Dodla, MilliporeSigma, US & Tomáš Kraus Ph.D., Institute of Organic Chemistry & Biochemistry AS CR, Czech Republic

About this talk

Imaging of live cells and tissues is a rapidly growing field and has revolutionized how biologists study cells, proteins and a multitude of processes and molecular interactions. Live cell imaging techniques allow scientists to observe cell structures, study dynamic processes and the localization of molecules in real time, and over time. The observation of dynamic changes provides more insight into the operations of a cell than a snapshot provided by imaging fixed cells. Moreover, the spatial resolution of light microscopy allows observing subcellular structures and cellular processes, by using a range of fluorescent probes. Hence, live cell probes are being used more frequently used, instead of antibodies. Moreover, since live cell imaging is less prone to experimental artifacts, it usually provides more reliable and relevant information than fixed cell microscopy. In this webinar researchers will: • Learn about some of the new live cell probes for cell structures, reactive ions, endocytosis, exocytosis, hypoxia, nucleotide transporters, and more. • Learn about probes for cellular stress by tracking reactive oxygen and nitrogen species in real-time. •Learn the benefits of imaging live cells as compared to fixed cells

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