Certified Reference Materials & Proficiency Testing in the 17025 laboratory

Peter Jenks, our Reference Materials and PT Business Development Specialist
Traceable reference materials. RTC has been producing environmental laboratory Proficiency Testing programs and certified reference materials for more than 20 years. More than 20,000 PT samples a year are sent to over 2,500 worldwide participants analysing soil, sediments and both waste and drinking water. This webinar will focus on how Sigma-Aldrich-RTC can support your laboratory with its PT and CRM needs.
May 29 2012
48 mins
Certified Reference Materials & Proficiency Testing in the 17025 laboratory
PT Proficiency Testing
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  • Practical Utilization of CRISPR in the Laboratory Workflow - Session 2 Recorded: Apr 22 2014 33 mins
    This 40 minute webinar will focus on the practical laboratory techniques needed to create and validate genetically modified cell lines using CRISPR. A Sigma-Aldrich® technology expert will discuss guidelines and techniques utilized when performing genome editing experiments.
    Topics will include:
    •Transfection of CRISPR reagent into a cell line
    •How to validate the activity level of CRISPR
    •Clonal isolation
    •How to screen clonal populations based on desired modification
  • Practical Utilization of CRISPR in the Laboratory Workflow - Session 1 Recorded: Apr 22 2014 30 mins
    This 40 minute webinar will focus on the practical laboratory techniques needed to create and validate genetically modified cell lines using CRISPR. A Sigma-Aldrich® technology expert will discuss guidelines and techniques utilized when performing genome editing experiments.
    Topics will include:
    •Transfection of CRISPR reagent into a cell line
    •How to validate the activity level of CRISPR
    •Clonal isolation
    •How to screen clonal populations based on desired modification
  • Preventing Culture Shocks: Understanding the Issues of Cell Line Authenticity Recorded: Mar 20 2014 54 mins
    Despite our dependence on cultured cells as models, issues surrounding cell identity, choice of appropriate cell lines, conditions of their growth and storage, microbial contamination and over-passaging are often overlooked or misunderstood. Mistakes can have devastating impacts on the legitimacy and reproducibility of science but potentially also, on careers, reputations and funding streams.

    Topics covered:

    Solutions and options for managing cell culture processes
    The Impact of misidentification
    The international consensus on cell identity and qualification
  • Genome Editing with CRISPR Systems - Session 2 Recorded: Jan 14 2014 62 mins
    CRISPR endonucleases have recently taken the research world by storm with their elegant and simple mode of RNA-guided gene targeting. CRISPR publications have flooded the literature in recent months, verifying that CRISPR endonucleases can operate via protocols developed for ZFN-based genome editing in human cells, rats, flies, worms, and plants (to name only a few).
  • Genome Editing with CRISPR Systems - Session 1 Recorded: Jan 14 2014 58 mins
    CRISPR endonucleases have recently taken the research world by storm with their elegant and simple mode of RNA-guided gene targeting. CRISPR publications have flooded the literature in recent months, verifying that CRISPR endonucleases can operate via protocols developed for ZFN-based genome editing in human cells, rats, flies, worms, and plants (to name only a few).
  • Proficiency Testing with Microorganism Standards Recorded: Dec 2 2013 13 mins
    A guide to using Vitroids from Sigma Aldrich as a tool for achieving laboratory efficiency
  • HPLC Method Development and Transfer Utilizing Fused-Core® Particle Technologies Recorded: Dec 5 2012 31 mins
    Superficially porous (Fused-Core, Core-shell) particle technologies have gained acceptance in general high-performance liquid chromatography (HPLC) and ultra-high pressure liquid chromatography (UHPLC) practice over the past several years due to improved efficiency relative to comparably sized fully porous particles. The Fused-Core option has also been shown to be a superior approach toward improving column efficiency as compared to smaller porous particle (sub-2 µm, UHPLC) technologies owing to the lessened backpressure penalties that are paid for the efficiency gains. Ascentis® Express HPLC columns were initially introduced that employed Fused-Core particles with an overall 2.7 µm diameter. These columns provide efficiencies equal to sub-2 µm particles at much lower backpressures as well as superior efficiencies when compared to fully porous 3 µm phases. There are still instances, however, where the backpressure generated by a 2.7 µm particle may preclude their use and there are also situations based on available equipment or regulatory guidance where larger particles are preferred. For these reasons, a new generation of 5 µm Fused-Core columns has been developed.
    In this seminar we will provide an introduction to the new line of Ascentis Express 5 µm HPLC columns and explore several scenarios practicing analytical chemists might encounter:

    • Desire to develop method on the more efficient 2.7 µm column, but need to be able to transfer to different location or lab
    1. Can I easily transfer methods from 2.7 µm to 5 µm?
    2. Can I easily transfer methods from 5 µm to 2.7 µm?

    • Wish to transfer methods based on fully porous column technologies to Fused-Core
    1. Can this be done easily?
    2. What gains should I expect? What can be done to optimize the gains?
    3. Can I transfer both 3 µm and 5 µm fully porous particle method to Fused-Core?
  • Evaluations of a New Ionic Liquid Stationary Phase with PEG Like Selectivity Recorded: Oct 31 2012 49 mins
    Geminal dicationic and polyionic ionic liquids have been prepared for use as stationary phases in capillary gas chromatography. These materials are known to provide higher thermal stability for gas chromatography, broader liquid working ranges and broader selectivity ranges than monocationic ionic liquids and polymeric based stationary phases with similar polarity. Recently, a new ionic liquid stationary phase has been developed that provides a selectivity very similar to a polyethylene glycol (PEG) selectivity but with increased thermal stability and lower bleed. We will compare and contrast the similar but unique selectivity of this new phase with traditional PEG phases using a wide variety of different sample types. We will demonstrate the improved thermal stability and lower bleed through a series of different studies and applications.
  • New! Introducing Ascentis Express 5 um HPLC Columns with Fused-Core Technology Recorded: Sep 21 2012 53 mins
    Core-type particles are competing strongly with small porous particles to improve the speed and resolution of HPLC and UHPLC experiments. The pioneering Ascentis Express column with Fused-Core® 2.7µm particles has exploded in popularity because it operates more ruggedly at much lower pressure than current sub-2µm porous particles, yet delivers the same ultra-high performance. This unique performance has been largely attributed to very narrow particle size distribution. Fused-Core® design advantages have also become popular for LC-MS because Ascentis Express columns surpass performance of columns with 3µm porous particles and operate ruggedly at higher velocities and similar pressures.

    With new, narrow-distribution 5µm Fused-Core® particles, the same design advantages can now be realized over traditional 5µm and 3µm porous particles that remain very popular for HPLC columns. An Ascentis Express 5µm column brings 3µm performance and extreme ruggedness at 5µm pressures to your laboratory. Like Ascentis Express 2.7µm particles, the 5µm particles show flatter van Deemter plots than same-size porous particles and allow separation speed to be maximized with minimal loss of resolution. Extremely high plates per pressure are observed. The core-type 5µm design should replace porous 5µm columns in routine HPLC applications with traditional instruments, and should also compete with porous 3µm columns in many LC-MS applications. Performance will be compared to 5µm and 3µm particle columns, and examples of method transfer will be shown. Ascentis Express 5µm will be available in the same phase modifications as the original Ascentis Express 2.7µm ultra-high performance column. Highly stable columns are available in various IDs and lengths up to 25cm.
  • Retention Mechanisms in HILIC Chromatography: Robust Method Development Recorded: Jun 28 2012 55 mins
    Hydrophilic interaction liquid chromatography (HILIC), especially in conjunction with mass spectrometry (MS), has become a powerful tool for the analysis of a wide variety of challenging analytes. Applications of the technique have increased dramatically over the past decade, especially for the analysis of polar analytes where reversed-phase chromatography suffers. HILIC conditions employ a high percentage of acetonitrile which enables facilitated solvent evaporation in LC/MS sources and thus often an increase in analyte response when compared to more aqueous based systems. The increased retention of polar analytes afforded by HILIC provides improved selectivity and decreases the impact of endogenous species, often leading to improved qualitative and quantitative analyses.

    Although HILIC has proven useful, it has also been thwarted with complications including difficulties in method development and method robustness.

    In this presentation, studies investigating the underlying retention mechanisms dominant in HILIC chromatography are presented and discussed. Along with reversed-partitioning HILIC is well known to exhibit, ion-exchange and the interplay of the dominant mechanisms are unveiled and used to develop a model of overall retention and selectivity. Interactions that operate using different stationary phase chemistries and conditions are presented. The impact of analyte polarity and charge as well as the variations caused by high percentages of organic on these physiochemical parameters are highlighted. Throughout the discussion, examples of use and misuse of HILIC are employed to illustrate these important concepts to build a solid fundamental foundation for efficient and effective use of this powerful technique.
  • Introduction to Air Monitoring Recorded: Jun 19 2012 60 mins
    Whether your laboratory uses passive sampling, or active air sampling, this webinar will run through the basics of air monitoring, giving some advice on the best equipment to use for your laboratories application.
  • Certified Reference Materials & Proficiency Testing in the 17025 laboratory Recorded: May 29 2012 47 mins
    Traceable reference materials. RTC have been producing environmental laboratory Proficiency Testing programs and certified reference materials for more than 20 years. More than 20,000 PT samples a year are sent to over 2,500 worldwide participants analysing soil, sediments and both waste and drinking water. This webinar will focus on how Sigma-Aldrich-RTC can support your laboratory with its PT and CRM needs.
  • Ionic liquid GC columns for the quantitation of trans fatty acids in fats & oils Recorded: May 16 2012 39 mins
    The fatty acid composition of a fat or oil is most commonly assessed by gas chromatography, following conversion of the fatty acids methyl esters. The current most refined analytical methods for the quantitation of trans fatty acids rely on the separations provided by long cyanopropyl siloxane capillary columns. The introduction of capillary columns coated with ionic liquids, such as Supelco SLB-IL111, provide an alternative separation tool characterized by a higher stationary phase polarity and selectivity toward geometric and positional isomers of unsaturated fatty acids. The use of these novel capillary columns can provide more refined separations of complex lipid samples. As a result, most conjugated linoleic acid isomers (including t7,c9- and c9,t11-18:1 FA) can be quantitated in a single separation using a 100 m SLB-IL111 capillary column and most 18:1 FA positional and geometric isomers can be separated using a 200 m SLB-IL111. Ionic liquid columns provide more detailed FA profiles, especially for unsaturated fatty acid positional/geometric isomers.
  • Introduction to Karl Fischer Titration Recorded: May 15 2012 62 mins
    As a leading authority on Karl Fischer Titration, we have extensive experience of product development and customer applications.
    Karl Fischer Titration is generally the most accepted method of water content determination and the webinar will provide an introduction to the technique.
  • Miniaturization of Sample Handling in Bioanalysis with SPME Recorded: May 9 2012 50 mins
    Bio-Solid-phase microextraction (BioSPME) is a simple, fast and sensitive non-exhaustive sample preparation technique that allows the integration of sampling and sample preparation steps. The objective of current research was to automate SPME in 96-well plate format for the first time. The proposed system allows sample preparation of >1000 samples/day, simultaneous determination of both free and total concentration and suitability for performing ligand-receptor binding studies. Open-bed configuration of SPME enables direct handling of heterogeneous matrices such as whole blood, thus further simplifying the entire sample preparation process. The automation of SPME in 96-well plate format enables the highest throughput of any SPME technique to date.
    In metabolomics studies of biofluids, the efficiency of metabolism quenching and stability of analytes in selected biofluid dictate how accurately the analytical results represent true metabolome composition at the time of sampling. However, complete quenching of metabolism is not easily accomplished and/or changes due to poorly stable compounds can occur, so the processes of sampling and sample preparation can significantly affect metabolome’s composition. The use of SPME for direct in vivo sampling of drugs and metabolites in the bloodstream of freely moving animals eliminates the need for blood withdrawal in order to generate pharmacokinetic (PK) or metabolomic profiles in support of pharmaceutical drug discovery studies. This is particularly important for situations with a limited blood volume such as mice because it enables the use of a single animal to construct an entire profile or conduct longitudinal studies. The aim of the current research was to apply SPME for in vivo sampling in mice for the first time. Furthermore, we investigate the use of in vivo SPME as an effective sample preparation method for both targeted pharmacokinetic and untargeted LC-MS metabolomics studies.
  • Introduction to SPME (Solid Phase MicroExtraction) Recorded: Apr 24 2012 69 mins
    SPME (Solid Phase MicroExtraction) is a solventless extraction technique, used to remove and preconcentrate low levels of volatile and semivolatile organic compounds from soil, water and air.
    This webcast will cover the basics of SPME; how to get started, and suggested applications of the technology.
  • Expanding the Capabilities of GC and MS with Ionic Liquids Recorded: Mar 21 2012 41 mins
    Ionic liquids (ILs) and their components have had a substantial impact on the capabilities and performance of gas chromatography, ESI-MS and MALDI-MS. For GC this ranges from the analysis of complex mixtures by GC x GC, water quantitation and food/beverage analysis among other areas. In MS the most sensitive analysis of anions, phospholipids and metal cations is often done by ESI-MS using complex IL cationic reagents. Also the best MALDI matrices for many proteins, peptides, carbohydrates and synthetic polymers are based on ILs. These will be discussed as time permits.
  • Introducing Ascentis Express ES-Cyano HPLC Columns Recorded: Nov 30 2011 37 mins
    10 minute introduction to the Ascentis Express family with an emphasis on the benefits of the new ES-Cyano phase. The presentation will then feature a 20 minute advanced level presentation on the molecular interactions contributing to alternative retention and selectivity using Cyano stationary phases
  • Using LC/MS to meet the challenges of a high through-put clinical diagnostic lab Recorded: Sep 21 2011 42 mins
    "With a BSc in Biochemistry and Molecular Biology from the University of Western Australia, I started my postgraduate career in the world of cell signaling and transgenic research at the Laboratories of Cancer Medicine at Royal Perth Hospital. After a number of years I relocated to the UK and started working in clinical diagnostic laboratories where I had a change of direction in my career. Moving into the development of HPLC and LC-MS/MS methods for clinical diagnostic analysis, I plied my trade at Guys Hospital and King’s College Hospital’s in London for 6 years before taking my current post at Addenbrooke’s hospital in Cambridge in late 2009. My work interests include new born screening, biogenic amines, steroids and vitamin analysis in an array of human sample types."
  • Analysis of Contaminants & Competition: Handling R&D Roadblocks Recorded: Jun 15 2011 29 mins
    Analytical R&D teams are thought of as a core facility within a company. On top of their regular R&D duties, they are often asked to solve production problems, ranging from identifying a contaminant to investigating a competitor’s new product. Learning how to approach these challenges and considering the use of resources beyond your laboratory can save both time and money.

    Three case studies will be presented. The case studies involve method development for sub-ppm detection, identifying an off-odor in food, and the detection of a low level pigment. A focus on the specific analytical technology will be presented in the case studies.
Focusing on new / innovative technologies and industry challenges
The Sigma-Aldrich seminar series features scientific presentations from key specialists in analytical chemistry, chemistry and life sciences, on the practical and technical aspects of new developments and innovations, to help advance your research.

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  • Title: Certified Reference Materials & Proficiency Testing in the 17025 laboratory
  • Live at: May 29 2012 1:00 pm
  • Presented by: Peter Jenks, our Reference Materials and PT Business Development Specialist
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