HPLC Method Development and Transfer Utilizing Fused-Core® Particle Technologies

Presented by

David S. Bell, Ph.D.

About this talk

Superficially porous (Fused-Core, Core-shell) particle technologies have gained acceptance in general high-performance liquid chromatography (HPLC) and ultra-high pressure liquid chromatography (UHPLC) practice over the past several years due to improved efficiency relative to comparably sized fully porous particles. The Fused-Core option has also been shown to be a superior approach toward improving column efficiency as compared to smaller porous particle (sub-2 µm, UHPLC) technologies owing to the lessened backpressure penalties that are paid for the efficiency gains. Ascentis® Express HPLC columns were initially introduced that employed Fused-Core particles with an overall 2.7 µm diameter. These columns provide efficiencies equal to sub-2 µm particles at much lower backpressures as well as superior efficiencies when compared to fully porous 3 µm phases. There are still instances, however, where the backpressure generated by a 2.7 µm particle may preclude their use and there are also situations based on available equipment or regulatory guidance where larger particles are preferred. For these reasons, a new generation of 5 µm Fused-Core columns has been developed. In this seminar we will provide an introduction to the new line of Ascentis Express 5 µm HPLC columns and explore several scenarios practicing analytical chemists might encounter: • Desire to develop method on the more efficient 2.7 µm column, but need to be able to transfer to different location or lab 1. Can I easily transfer methods from 2.7 µm to 5 µm? 2. Can I easily transfer methods from 5 µm to 2.7 µm? • Wish to transfer methods based on fully porous column technologies to Fused-Core 1. Can this be done easily? 2. What gains should I expect? What can be done to optimize the gains? 3. Can I transfer both 3 µm and 5 µm fully porous particle method to Fused-Core?

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