High-Definition Spatial Proteomics for Characterizing the Tumor Microenvironment

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Presented by

Jason Ptacek, PhD, Associate Director of Research Services, IONpath, Inc.

About this talk

The complexity of the TME with variable cellular composition and organization has driven the development of experimental techniques capable of tracking large numbers of biomarkers in tissue with sub-cellular resolution. This webinar will provide an overview of how high-definition spatial proteomics, enabled by Multiplexed Ion Beam Imaging (MIBI), can be used to explore the tumor microenvironment (TME) of tumor biopsy samples with unprecedented depth. MIBI leverages the high resolution of a rastered primaryion beam and the sensitivity of time-of-flight secondary ion mass spectrometry (ToF-SIMS) to image standard frozen or formalin fixed paraffin embedded (FFPE) tissue sections that have been stained with up to 40 different antibodies labeled with metal isotope tags. Masses of detected species are assigned to target biomolecules given the unique label of each antibody. Multi-step processing and segmentation utilizing highly expressed nuclear, membrane, and cytoplasmic markers are then performed to accurately determine cell boundaries. Cell classification is performed to phenotypically characterize the tissue environments and quantify marker expression. Dr. Jason Ptacek, Associate Director of Research Services at IONpath, Inc., will share examples of quantitative single-cell phenotype mapping in tissue analysis applications and review the data analysis methods used to generate actionable insights from this highly multiplexed, spatial proteomic data. Learn how MIBI provides: ● Comprehensive, quantitative single-cell phenotyping across tumor samples ● Characterization of the spatial organization of cell populations of the TME ● Sensitivity to quantify the expression of markers associated with immune suppression

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