Understanding the cellular heterogeneity of tissues such as tumors is crucial for both translational and clinical research. As a result, highly focused techniques such as single-cell RNA sequencing (scRNA-seq) and ultra-low-input mRNA-seq are expanding rapidly to overcome the challenges that single cells and restricted cell populations pose for library preparation and reliable transcriptome analysis.
In many scRNA-seq workflows, the initial steps employ the template switching method for cDNA synthesis followed by cDNA normalization prior to library preparation. Roche’s KAPA HyperPrep Kit is effective across a 30-fold range of cDNA input amounts, eliminating the need for cDNA normalization in scRNA-seq workflows while also generating complex, uniform libraries and high-quality sequencing data.
In this webinar, we will:
- Present an ultra-low-input workflow that yields high-quality libraries from as little as 10 pg of input RNA using the template switching method for cDNA synthesis followed by library preparation using KAPA HyperPlus Kit
- Demonstrate the advantages of the KAPA HyperPlus Kit over the tagmentation-based methods, including greater flexibility on cDNA input quantities
- Present scRNA data from libraries prepared with KAPA HyperPlus Kit