Ultra-low input and single-cell mRNA-seq library prep using KAPA HyperPlus Kit

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Presented by

Mariana Fitarelli Kiehl, PhD, Senior Applications Scientist at Roche Sequencing and Life Science

About this talk

Understanding the cellular heterogeneity of tissues such as tumors is crucial for both translational and clinical research. As a result, highly focused techniques such as single-cell RNA sequencing (scRNA-seq) and ultra-low-input mRNA-seq are expanding rapidly to overcome the challenges that single cells and restricted cell populations pose for library preparation and reliable transcriptome analysis. In many scRNA-seq workflows, the initial steps employ the template switching method for cDNA synthesis followed by cDNA normalization prior to library preparation. Roche’s KAPA HyperPrep Kit is effective across a 30-fold range of cDNA input amounts, eliminating the need for cDNA normalization in scRNA-seq workflows while also generating complex, uniform libraries and high-quality sequencing data. In this webinar, we will: - Present an ultra-low-input workflow that yields high-quality libraries from as little as 10 pg of input RNA using the template switching method for cDNA synthesis followed by library preparation using KAPA HyperPlus Kit - Demonstrate the advantages of the KAPA HyperPlus Kit over the tagmentation-based methods, including greater flexibility on cDNA input quantities - Present scRNA data from libraries prepared with KAPA HyperPlus Kit

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Next-generation sequencing (NGS) is driving advances in translational and clinical research. From expanding our understanding of cancer, reproductive and developmental biology to unravelling complex microbial populations, NGS is touching all facets of human disease and well­being. Sample prep for NGS holds the key to unlocking the potential of every sample. Explore our integrated Roche Sample Preparation Solutions, encompassing all the steps required to convert a sample to a sequencing-ready library. From sample collection to library quantification, we offer sample prep solutions for different sample types and sequencing applications that are proven, simple, and complete.