The Application of NGS in CRISPR Gene Editing & Single-cell Analysis

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Presented by

Jason Liu, PhD, Field Applications Scientist at Roche Sequencing and Life Science

About this talk

Accurate DNA amplification is essential for NGS-based workflows, including CRISPR gene editing, screening of CRISPR clones for on-target and off-target genome modifications, and single-cell RNA sequencing (scRNA). The robust, high-fidelity KAPA HiFi polymerase enables fast, efficient workflows for each of these methods. In this webinar, we demonstrate how KAPA HiFi DNA polymerase and KAPA NGS Library Preparation Kits enable robust workflows for: - CRISPR/Cas-9 technology for genome editing - Indel screening following CRISPR/Cas-9 editing - CIRCLE-seq, a QC methodology for identifying CRISPR/Cas-9 off-target cleavage sites - Single-cell RNA sequencing (scRNA-seq) MC-US-09264

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Next-generation sequencing (NGS) is driving advances in translational and clinical research. From expanding our understanding of cancer, reproductive and developmental biology to unravelling complex microbial populations, NGS is touching all facets of human disease and well­being. Sample prep for NGS holds the key to unlocking the potential of every sample. Explore our integrated Roche Sample Preparation Solutions, encompassing all the steps required to convert a sample to a sequencing-ready library. From sample collection to library quantification, we offer sample prep solutions for different sample types and sequencing applications that are proven, simple, and complete.