Name: Tips and Tricks to Help Develop and Run Microplate-based Assays
Start: 2014-09-25T16:00:00Z
End: 2014-09-25T16:58:11.000Z
Location: BrightTALK
Rating: 4.4

The Corning Scientific Seminar Series is a series of free, online technical presentations that provide novel tips, best practices and proven techniques to help advance your research. Delivered by scientists to scientists, these one-hour broadcasts offer useful information and tips for lab technicians and researchers.

This webinar will demonstrate the use of the Corning Matribot Bioprinter to accurately and consistently dispense pancreatic cancer organoids for drug testing. Dispensed organoid cultures were assessed for toxicity to several chemotherapeutics that are traditionally used for treating pancreatic cancer. This data demonstrates an automated way to screen for the best drug choice for an individual’s pancreatic cancer.

Corning® Matribot® Bioprinter for Organoid Assays

This March, Corning Life Sciences is celebrating our female leaders in STEM. As of 2019, women made up 38% of all STEM workers, and that number is on the rise. Hear from Elizabeth Abraham on her experiences starting a career as a woman in STEM, and how she has grown within it.

Growth and Diversity with Careers in STEM

In this webinar, we will detail regulated medical waste (RMW) management innovations including:
-A comparative review of traditional RMW management practices 
-An exciting, new, environmentally friendly treatment alternative for RMW with no hazardous emissions
-Opportunities for significant sustainability improvements in managing RMW, including reliable, safe, efficacious treatment and reclamation of targeted plastic constituents.

Ozone: The Future of Medical Waste Management and More?

Investigating the relative contribution of mutations, nutrient availability, and 2D vs. 3D growth on the behavior of cancer cells.

This webinar covers:
-Constructing a genotypic series of colon cancer organoids
-Characterizing  3D organoids vs. isogenic 2D cell lines
-Mutations, Media, and 2D vs. 3D. What determines the metabolic state of cancer cells?

Tutorial: Genes or Environment? Investigating the Behavior of Cancer Cells

A simplified approach for rapid analysis of thousands of intact spheroids using the Velocyt® LP Large Particle Flow Cytometer.

The Velocyt address the issues of 3D multicellular models: big objects, intact analysis, high sample numbers and total sample recovery. Using Corning® Elplasia® technology simplifies the Velocyt workflow and increases assay robustness and reproducibility.

Tutorial: Rapid Analysis of Intact Spheroids using the Velocyt® LP Cytometer

Throughout the medical community, there is a growing concern about the rising costs for bio-hazardous waste remediation. Organizations have begun to examine the effects on landfills, and the impacts of incineration, originating from this type of waste. Most organizations today are seeking more effective and new approaches to this critical process outside of the standard practice of removal and destruction of the bio-hazardous waste by a 3rd party vendor, but the options have been limited – until now. 

To combat this ever-expanding problem of RMW, Sterilis Solutions sought a solution that addressed all the impacts detailed above. The solution came in the form of their product, the Remediator. The Remediator, which is no bigger than a standard office copier, combines a steam sterilizer and grinder into a single unit. In a few simple steps, the biohazardous waste found in sharps containers and biohazard bags is reduced to what is essentially harmless confetti. It can then be entered into an advanced recycling program.

An Alternative Approach to Regulated Medical Waste (RMW) Hauling

Once thought to be merely evictees of cell debris, extracellular vesicles (EVs) hold the promise of the next generation therapeutic agent. EVs are known cell-to-cell messengers that mediate physiological functions throughout the body. Their ability to stealthily transfer paracrine factors, nucleic acids, proteins, and other essential biomolecules between distant tissues have the potential to usher in a new era of nanomedicine.

This webinar will focus on why stem cell derived EVs are a compelling acellular therapeutic. We will discuss the unique characteristics of EVs compared to other synthetic therapeutic agents and outline the critical attributes necessary to be a successful regenerative medicine alternative to whole stem cell therapies. We will explore how naïve EVs are produced, isolated, and characterized with an emphasis on emerging approaches in characterization and quantification methods. Lastly, we will review the application of EVs as natural therapeutic agents in the clinic, which include regenerative medicine, clinical oncology, and COVID-19 therapies.

Extracellular Vesicles: From Waste EVictees to Therapeutic Reality

Cell culture media is one of the most important components of the in vitro tissue environment. It provides the nutrients, minerals and growth factors necessary to sustain cells outside the body.

This Teach Me in 10 explores the different media options available for plating mammalian cells. It covers preparation techniques, storage options and how to choose the right media for your experiment.

Plating Cells: Preparation and Media Options With Ann Rossi Bilodeau

The field of gene and cell therapy has experienced rapid growth over the past decade, and the expansion of the number of therapeutics coincides with the growth of the scale of manufacturing. Through the seed train process, manufacturing begins with a few million cells and ultimately produces billions of cells to serve patient populations. With so much material on the line, researchers face the task of minimizing risks and maximizing product quality. Closed systems in conjunction with bioreactor systems provide a controlled and monitored environment that can not only enhance cell performance, but also provide a scalable path from process development to manufacturing. This seminar will review adherent cell culture scale up with attention to the following topics:
- Considerations for seed train optimization 
- Considerations for closed system process development and manufacturing
- Adherent cell culture bioreactor systems from Corning, including the Ascent® FBR

Amping Up Yield and Enhancing Quality in Adherent Cell Culture Scale-Up

Novel gene therapies are entering the clinic and generating clinical data readouts at an unprecedented rate. As with any new therapy, the key readouts are safety and efficacy. While there has been substantial progress and clinical success, the gene therapy field must continue to refine the engineering of viral vectors for increased manufacturability, targeted efficacy, and safety.

Exclusively designed for experts working in the Gene Therapy space, this exclusive 1-hour symposium will allow you to:
-Get up to speed with AI and Machine learning for vector engineering and how it can optimize viral vectors
-Discover how to enhance therapeutic efficacy with targeted tissue and cell expression
-Delve into the impact of transgene cassette optimization to understand the next generation of viral vector manufacturing

Gene Therapy 2.0: Leveraging Transgene Cassette Engineering

Advances in the science of gene and cell therapies have created rapid growth in the industry, with the common challenge of producing therapeutic cells or viruses at manufacturing scale. Corning has developed a novel fixed bed bioreactor system to address the need for more efficient solutions for cell and gene therapies as well as vaccine production. This webinar covers:
• Challenges in scaling up stem cells
• How the Corning Ascent FBR System enables high yields of cells and viral vectors through its unique fixed bed design
• How efficient cell-harvest, automation, process control and ready-to-use consumables will improve your adherent bioproduction

Corning® Ascent® FBR System — Technology for Scalable Adherent Cell Culture

Cells cultured in controlled conditions including temperature, gas exchange, pH, and culture media (energy and nutrients) are the foundation of many research and production applications within the life sciences industries. This webinar will introduce basic concepts of cell culture and provide information on pathways to success for culturing mammalian cells. 
 
In this webinar: 
 
1. What is cell culture? 
2. What is the difference between primary and cell line and/or adherent and suspension cell? 
3. Introduction to a variety of culture vessels for adherent and suspension cells. 
4. How can we choose correct surfaces for your cell types? 
5. Troubleshooting cell culture.

Fundamentals of Cell Culture

血球計算盤を用いた細胞数の計数方法についてデモ動画を用いて解説し、得られた細胞数から増殖曲線を作成して倍加時間を算出する方法も説明します

第4回　細胞計数と増殖曲線

Advances in the science of gene and cell therapies have created rapid growth in the industry, with the common challenge of producing therapeutic cells or viruses at manufacturing scale. Corning has developed a novel fixed bed bioreactor system to address the need for more efficient solutions for cell and gene therapies as well as vaccine production. 
In this webinar you will learn how the Corning Ascent FBR System enables high yields of cells and viral vectors through its unique fixed bed design. Further, we will cover how efficient cell-harvest, automation, process control and ready-to-use consumables will improve your adherent bioproduction.

Corning® Ascent® FBR - Innovative technology for scalable adherent cell culture

Single-use technologies in biopharmaceutical manufacturing is rapidly expanding these days due to the several advantages including the minimized risk of contamination, less facility needs, capital costs reducing, and faster time to market. Corning has delivered series of solutions that have advanced the science of suspension culture in the past decades. 
In this webinar, learn more about Corning’s Suspension Culture Flasks and how our unique design of flasks optimizes cell expansion and production value.

Efficient expansion of suspension cells with Corning solutions

培養細胞の選択から入手方法まで説明し、また入手後の凍結保存ストックの作製と品質管理についてデモ動画を用いて解説します

第3回　細胞の入手と品質管理

This session will demonstrate the use of the Corning Matribot Bioprinter to accurately and consistently dispense pancreatic cancer organoids for drug testing. Dispensed organoid cultures were assessed for toxicity to several chemotherapeutics that are traditionally used for treating pancreatic cancer. This data demonstrates an automated way to screen for the best drug choice for an individual’s pancreatic cancer.

Corning® Matribot® Bioprinter Printed Domes for Organoid Drug Testing

The Corning Elplasia Flask is a new high-density static cell culture platform that can support the generation of ~12,000 spheroids in a footprint similar to that of a T-75 flask. Learn how the Elplasia Flask supports 3D cell culture model breakthroughs that 2D cell culture simply cannot.

CLS Practicum: Corning® Elplasia® Flask

Are you relying on biochemical and cell based in vitro assays in your research? In this webinar, Dr. Mark Rothenberg will share “insider” tips and tricks for enhancing your biochemical and cell-based assay performance.
Critical parameters for achieving success with these assays include understanding the assay dynamics, the instrumentation involved in reading the assay, and, in the case of cell-based assays, the environment in which the cells are grown impacts cellular physiology. Factors such as microplate geometry, density, surfaces, and instrumentation all play important roles in the success of the assay.
Learn how to: 
•Select the correct microplate and its role in the success of the assay
•Choose the correct tip for your liquid handling needs
•Determine the correct instrumentation and settings to run your assay

Tips and Tricks to Help Develop and Run Microplate-based Assays

Microplate

Assay

Corning

cell

culture

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Tips and Tricks to Help Develop and Run Microplate-based Assays

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