Tips and Techniques for Culturing and Scaling-Up Primary Cells

Presented by

Mark Rothenberg, Ph.D.

About this talk

Primary cells can more closely mimic an in vivo-like state and generate more physiologically relevant data than immortalized cells. But unlike immortalized cells, primary cells have complex nutritional needs and require optimized growth conditions. In this webinar, you will learn: •Proven techniques for isolating primary cells from tissues •The importance of choosing the correct dissociation method •Troubleshooting techniques for culturing healthier primary cells. •Other topics to be discussed include: -Choosing the optimal cell culture surface -Selecting the optimal cell culture media  -Choosing the correct vessel for scaling-up

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The Corning Scientific Seminar Series is a series of free, online technical presentations that provide novel tips, best practices and proven techniques to help advance your research. Delivered by scientists to scientists, these one-hour broadcasts offer useful information and tips for lab technicians and researchers.