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Your In Vitro ADME CRO: New Assays for Transport, Enzyme Inhibition & Induction

Achieving your ADME/Tox testing goals requires experience, quality data, and proper alignment with regulatory guidance. Failure to meet these important requirements can put your drug discovery and pre-clinical goals at risk.
This presentation will provide an informative overview of how you can advance and reach your pre-clinical drug discovery goals. It will discuss the importance of core contract research capabilities, including enzyme induction, enzyme inhibition, and transporter interaction. In addition, we will review new capabilities and opportunities including CYP induction and SLC transporter assay services – all designed to align with regulatory agency guidance documents.

Speaker Bio:
David Stresser is the Program Manager of Corning® Gentest℠ Contract Research Services at Corning Life Sciences since 2001, having held prior positions of Product Manager and Study Director since joining Corning in 1998. Prior to this, he was a post-doctoral associate in the laboratory of David Kupfer at the University of Massachusetts Medical School in Worcester, Massachusetts. He did his graduate work in the laboratory of David E. Williams at Oregon State University in Corvallis, Oregon receiving a Ph.D. in toxicology in 1994. Dr. Stresser has authored or co-authored 40 articles or book chapters in the field of drug metabolism and has been an invited speaker at various national and international meetings, pharmaceutical companies, and universities.
Recorded Jul 29 2015 60 mins
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David M. Stresser, Ph.D.
Presentation preview: Your In Vitro ADME CRO: New Assays for Transport, Enzyme Inhibition & Induction

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    Looking for more great Matrigel Matrix tips and tricks? We’ve summarized a bunch in our newly published "Ultimate Guide to Matrigel Matrix" – download it today: https://goo.gl/9Nq5Td
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    コーニングインターナショナル株式会社 ライフサイエンス事業部 石渡孝至
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    By attending this webinar you will learn about:
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    • Ways to enhance imaging and characterization of 3D spheroids

    Presenter Bios:
    Dr. Tom Villani is the CSO and Co-founder of Visikol Inc and is responsible for the companies scientific strategy. Since launching Visikol with Co-Founders Dr. Michael Johnson and Nick Crider, Dr. Villani has led the development of the Visikol HISTO tissue clearing technology for three-dimensional tissue imaging as well as a suite of digital pathology tools. Visikol has leveraged these technologies in its 3Screen service offering where the company is focused on transforming tissues into actionable insights as a service for primarily pharmaceutical companies.

    Dr. Ann Rossi graduated from the University of Rochester School of Medicine and Dentistry with a Ph.D. in Pharmacology and received postdoctoral training at the University of Chicago. Prior to joining Corning, Ann worked as a Senior Scientist at ARMGO Pharma, Inc., a small private pharmaceutical company, contributing her expertise in calcium signaling toward developing new assays for the company’s screening cascade. Ann is new to Corning Life Sciences as the Applications Lab Manager in Kennebunk, Maine and is drawing on her strong academic and industry research experience to direct the activities of the applications group.
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    Samuel Constant, PhD, Co-founder, CEO
    In this special webinar, our guest presenter Samuel Constant Ph.D., Co-founder, CEO for OncoTheis will review:

    - Novel in vitro tests for modelling lung cancer
    - A model that allows long term monitoring of toxicity or efficacy on respiratory tract
    - How OncoCilAir™ is a 3D human airway epithelium with tumors reconstituted in vitro

    Abstract:
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  • Modeling NAFLD and TGFβ-induced Fibrosis in 3D Bioprinted Human Liver Tissue Recorded: Oct 5 2017 52 mins
    Jeff Irelan, Ph.D.
    We hope you join us for this special webinar. Jeff Irelan, Director of Scientific Applications for Organovo will be our guest presenter.

    Abstract:
    Nonalcoholic fatty liver disease (NAFLD) is the most common liver disorder with an estimated prevalence of over 25% worldwide and is projected to become the leading indication for liver transplant by 2025. Despite decades of research focused on NAFLD, an effective treatment has yet to be approved. This is due in part to the reliance on cell culture and animal models that present challenges in translation due to limited functional longevity and species differences, respectively.

    ExVive™ 3D Bioprinted Human Liver Tissue, a clinically-translatable in vitro model, is ideal for studying the effects of drugs on liver disease progression, regression, and the mechanisms involved. Here, we present results showing a nutrient overload induction of liver disease and TGFβ-induced fibrosis in ExVive™ Human Liver Tissue. A variety of disease-relevant phenotypes including steatosis, inflammation, and fibrosis can be demonstrated in the model:
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    •A TGFβR1 kinase inhibitor effectively blocks TGFβ-induced fibrosis.

    Presenter Bio:
    Jeff Irelan holds a Ph.D. in molecular biology from the University of Oregon. As Director of Scientific Applications, Jeff interfaces with Organovo’s customers and R&D team to implement and expand the company’s portfolio of service offerings utilizing bioprinted tissue models.
  • New Technologies for Cellular Research: 3-Dimensional Cell Culture and Screening Recorded: Sep 22 2017 54 mins
    Richard M. Eglen, Ph.D
    It is now recognized that target and compound identification, as well as validation, are better conducted using cells with physiologically relevant phenotypes and genotypes. This assertion has accelerated the adoption of primary cells, stem cells, or patient-specific cells in cellular research, in general; and drug discovery, in particular.

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    In this presentation, the existing and future impact of 3D cell culture technology on fundamental research, and drug discovery and manufacture will be addressed, particularly in the context of using phenotypically relevant cells. Specifically, it will discuss the potential for spheroids, organoids, scaffolds, and hydrogels in cellular research and compound identification, screening, and development.

    Future directions will also be covered, including organs-on-chips, hydrostatic flow technologies, microfluidics, and 3D bioprinting. Some of these approaches will allow for real-time observation of cellular responsiveness to novel compounds and drugs … boldly taking the researcher into a fourth dimension of 3D cell culture!
  • 浮遊系細胞培養用シングルユースシステム Recorded: Sep 12 2017 60 mins
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    近年、バイオ医薬品開発に於けるシングルユース製品の市場が活況を呈しております。抗体ワクチン、幹細胞/ヒトiPSや浮遊系細胞による製剤化やバイオバンキン グを具現化する際に、最も大きな懸案事項となりますのが“質”、“量”そして“コスト”の問題があります。そこでコーニングでの安全、安価かつ簡便なシングルユース製品の特徴や仕様についてご紹介致します。
     また治験薬に向けた品質規格や導入事例によるマーケットトレンドを示しながら、トータルソリューションシステムの振盪培養(フラスコ、べセル、バッグ)、チュービング&コネクション(閉鎖系システム、無菌接続)、パッケージング(培養 / 回収 / 凍結保存)および品質規格(施設環境、BSE/TSE、発熱物質(Pyrogen)、USPクラス6規格準拠 / 滅菌保証レベル / エンドトキシン規格 / QC試験)について概説します。
  • バイオ医薬品製造におけるシングルユースシステム Recorded: Jun 6 2017 77 mins
    コーニングインターナショナル株式会社ライフサイエンス事業部 石渡孝至
    バイオロジクスの治験薬製造におけるシングルユース技術導入ポイントであります製品ソリューション、クローズドシステムおよび品質保証体制について概説します。

    再生・細胞医薬を中心にバイオ医薬品の製造開発が急速に進められております。

    幹細胞やヒトES / iPS細胞による再生医療を具現化する際に、最も大きな懸案事項となりますのが“質”、“量”そして“コスト”の問題があります。

    そこでコーニングは、安全、安価かつ簡便な最新シングルユース技術についてレギュレーション、導入事例によるマーケットトレンドを示しながら、トータルソリューションシステムのセルカルチャー(多層式大量培養容器)、チュービング&コネクション(プレアッセンブル特注、無菌接続)、パッケージング(培養 / 回収 / 凍結保存)、データインテグリティ(バーコードによるトレーサビリティ管理)、品質規格(成型施設環境、BSE/TSE、発熱物質(Pyrogen)、USPクラス6規格準拠 / 滅菌保証レベル / エンドトキシン規格 / QC試験)および品質に関するドキュメンテーションサポート(証明書、バリデーションバインダ)に関する情報を概説します。
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    Van Dang, D.V.M., Ph.D., Abhi Saharia, Ph. D., and Audrey Bergeron, Applications Scientist
    Webinar Date & Time: Mar 30 2017 12:00 p.m. EST

    Chimeric antigen receptor (CAR)-T cells, which are engineered to recognize target cell surface antigens expressed on tumor cells, have shown promise to affect complete remission in patients with B-cell malignancies. However, applying this approach to target solid tumors has resulted in adverse effects in clinical studies. Methods for testing different models of CAR-T cells in vitro can provide further insight into viable antigen targets. Historically, two-dimensional (2D) cell culture models have been used in drug discovery. However, more elaborate, three-dimensional (3D) cell culture models better mimic the in vivo tumor microenvironment and help bridge the gap between in vitro studies and clinical outcomes.

    In this special joint webinar, panelists from ProMab Biotechnologies, DiscoverX, and Corning Life Sciences will present data on a high-throughput, easy-to-use, highly reproducible method for screening CAR-T cells in a 3D cell culture model by combining various technologies.


    Speakers:
    Van Dang, D.V.M., Ph.D.
    Scientist and coordinator for CAR-T research
    ProMab Biotechnologies, Inc.

    Abhi Saharia, Ph. D.
    Director, Cell-based Assays and Biologics
    DiscoverX

    Audrey Bergeron
    Applications Scientist
    Corning Life Sciences
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    Nitin Kulkarni, Ph.D.
    3D culture is gaining pivotal importance for attaining in vivo-like conditions in a dish to study developmental cues as well as therapeutic possibilities. Organoid development promises to be one of the most important research tools in the near future. This presentation will cover:

    • Methodologies used in organoid culture
    • Matrices for growing organoids
    • Recovery of organoids for downstream applications

    Speaker Bio:

    Dr. Nitin Kulkarni is a member of the Scientific Support team at Corning Life Sciences. He has a Ph.D. in Biology and has worked on engineering transgenic mouse models for autoimmune diseases during his post-doctoral research at the Beth Israel Deaconess Medical Center in Boston, MA.
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  • Rescheduled: Multicellular Tumor Spheroids in HTS: New Assays Recorded: Feb 1 2017 50 mins
    Wojciech Senkowski
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    In summary, this webinar will demonstrate new ways of how MCTS-based HTS can be used to provide unique insights into context-dependent biology and cellular drug responses.

    About the Presenter:

    Wojciech Senkowski will soon complete his Ph.D. in Medical Sciences at Uppsala University, Sweden. In his work, he looks for applications of various tumor spheroid models in high throughput drug screening. For his work, Wojciech has received the AACR Scholar-in-Training Award. He was also a presenter and expert panelist at the Genetic Engineering & Biotechnology News webinar on 3D cell cultures, sponsored by Corning in February of 2016.
  • The Impact of Soluble Factors and Substrate on Cell Culture Recorded: Nov 3 2016 48 mins
    Kevin Kelly
    Webinar: The Impact of Soluble Factors and Substrate on Cell Culture: Media Additives, Growth Factors, and Surface

    From basal media with feeder layers or serum to highly defined recombinant growth factors, cytokine, and extracellular matrix, there are many ways to grow the same cell type. The choice is dependent on scale, cost, control, skill, and regulatory factors.

    This webinar will cover:
    - Different ways to grow the same cell type
    - The actual material costs of various methods
    - Methods used to optimize formulations

    Speaker Bio:
    Kevin Kelly graduated from Hawaii Pacific University and for 15 years worked on process scale-up and optimization for extracellular matrix proteins, growth factors, cytokines, antibodies, ELISA kits, and Corning® BioCoat™ products.

    Currently he provides applications support for invasion, migration, permeability, transport, differentiation, and metabolism assays.
  • Current Trends in 3D and Organoid Cell Culture for Cancer Research Recorded: Oct 4 2016 67 mins
    Marshall Kosovsky, Ph.D., Ömer H. Yilmaz, M.D., Ph.D.,Wojciech Senkowski,
    Corning was pleased to have recently sponsored a GEN webinar highlighting the latest techniques for 3D cell culture in cancer research.

    The use of 3D cell cultures has been rising sharply in recent years from its initial introduction, over two decades ago. Because 3D cultures more accurately mimic the cellular environment, they can be used to study various forms of cancer by fostering the growth of organoids that replicate key properties of in vivo organ systems or the original tumors from which they were derived.

    In addition, many 3D cultures are amenable to large-scale drug screens for rapid detection of phenotypic or genetic changes associated with therapeutic compounds—an approach that opens the door for the use of 3D culture as an integral part of personalized medicine.

    In this GEN webinar, panelists discussed how the latest 3D cell culture methods have facilitated breakthroughs in their research projects.

    Panelists:
    Marshall Kosovsky, Ph.D., Global Scientific Support Manager for Corning Life Sciences, will give a brief introduction into advances in Corning’s 3D culturing solutions.

    Ömer H. Yilmaz, M.D., Ph.D., Assistant Professor of Biology at the Massachusetts Institute of Technology, will describe his work on how adult stem cells and their microenvironment adapt to diverse conditions within the context of tissue regeneration and cancer initiation through the use of ex vivo intestinal organoid assays.

    Wojciech Senkowski, Doctoral candidate in the Department of Medical Sciences at Uppsala University in Sweden, will discuss his current work, which looks for applications of various tumor spheroid models in high-throughput drug screening for ways to identify novel compounds that target these cell populations
  • Using Microcarriers to Speed-up Your Scale-up. Recorded: Sep 15 2016 33 mins
    Jennifer Weber
    This presentation describes critical factors for selecting microcarriers, as well as starting protocols to help you optimize the attachment and expansion of cells in spinner flask and bioreactor environments.

    In addition, you’ll learn about new dissolvable microcarrier technology, which provides unique advantages for cells that cannot be easily separated from standard microcarriers.

    Speaker Bio:

    Jennifer Weber is a senior development scientist with Corning Life Sciences. She has helped develop a variety of products for culturing advanced cell types including Corning® Synthemax™, a synthetic, xeno-free surface, and Corning stemgro ® hMSC, a serum-free, chemically defined medium for hMSC culture.

    She recently transitioned to microcarrier product development and customer support for bioprocess applications. As part of this role, she facilitates customer adoption of Corning products for specific applications through protocol development, on-site technical support, and in-house customer-driven projects.
  • Understanding Cell Culture Media Composition to Improve Outcomes Recorded: Jul 28 2016 59 mins
    Brian Posey
    Cell culture media is required for successful and reproducible research but the catalog is full of acronyms and various formulation tables. Classical mammalian cell culture media formulations are very diverse both in terms of the number available and the concentration of constituents. Additionally, each medium was designed for specific cell types and culturing conditions.

    This webinar will cover:
    •The composition, characteristics, environmental factors, and additional supplements required to create optimal conditions for growth and productivity.
    •Determining the right formulation for your application.
    •Serum usage and helpful tips for optimizing your culture conditions.

    Speaker Bio:
    Brian Posey is a Product Development Manager for cell culture media at Corning Life Sciences. Brian has over 10 years experience in cell biology and industrial scale cGMP manufacturing of both liquid and powder cell culture media. Since joining Corning in 2012, Brian has led numerous innovative technology projects for the media business ranging from customer technology transfer for production scale-up to developing new serum-free media for industrial and stem cell lines.
  • In Vitro Characterization of Species Difference of OATP Recorded: May 25 2016 52 mins
    Na Li, Ph.D.
    Organic anion-transporting polypeptides (OATPs) play an important role in hepatic uptake of a variety of clinically important drugs. The significant differences in OATP/Oatp-dependent drug transport between human and preclinical species presents a challenge for interspecies extrapolation of drug pharmacodynamics and pharmacokinetics. The assessment of the difference in hepatic uptake between species using an in vitro model is highly desired to support mechanistic studies and to understand the differences observed between species in vivo.

    The Corning® TransportoCells™ model has demonstrated significant value in terms of supporting in vitro assessment of drug interaction with SLC transporters in drug discovery and development. Recently, several animal species of Oatps were introduced into the Corning TransportoCells portfolio. This webinar will focus on the development of the newly available animal species and characterization of the differences in OATP/Oatp in substrate specificity and kinetics using this "thaw and go" model.
Training for Life Science Researchers
The Corning Scientific Seminar Series is a series of free, online technical presentations that provide novel tips, best practices and proven techniques to help advance your research. Delivered by scientists to scientists, these one-hour broadcasts offer useful information and tips for lab technicians and researchers.

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  • Title: Your In Vitro ADME CRO: New Assays for Transport, Enzyme Inhibition & Induction
  • Live at: Jul 29 2015 4:00 pm
  • Presented by: David M. Stresser, Ph.D.
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