Specialized Applications Using the Corning® Spheroid Microplate

Presented by

Hilary Sherman, Applications Specialist & Audrey Bergeron, Application Specialist

About this talk

Three-dimensional (3D) cell culture models, which offer significant improvements over traditional two dimensional monolayer cell culture in terms of maintaining morphological and functional characteristics of tissue, are increasingly being incorporated in drug discovery as model systems to study disease or for screening for chemotherapeutic efficacy or drug toxicity. This presentation will demonstrate the use of Corning® spheroid microplates to culture and assay spheroids in a rapid and highly reproducible format that enables the formation of a single multi-cellular spheroid, centered in each well. Corning spheroid microplates are multiple well, cell culture plates with opaque walls and unique clear, round well-bottom geometry that utilize the Corning Ultra-Low Attachment surface coating. The coating is hydrophilic, biologically inert and non-degradable. Representative data will be shown exhibiting the use of the spheroid microplate in more specialized assays including the formation of neurospheres from neural stem cells (NSCs), a valuable model to study neurogenesis and neural development, as well as analysis of NSC proliferation and migration. We will also demonstrate the formation of hepatospheres using Corning HepatoCells, an immortalized alternative to primary human hepatocytes, in combination with the SCREEN-WELL Hepatotoxicity library from Enzo Life Sciences for 3D hepatotoxicity screening. Finally, we will show the use of the Corning spheroid microplate to generate spheroids comprised of multiple cell types, demonstrating the impact that including multiple cell types in 3D assays can have on therapeutic outcome in a chemotherapeutic assay.

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