Hi [[ session.user.profile.firstName ]]

Ask the Experts: 3D Cell Culture and Assays

3D culture experts from Corning and Promega answer questions about common methods to form 3D structures, and factors to consider when evaluating cell-based assays for use with 3D structures. Learn about the novel viability and cytotoxicity assays that do not lyse cells and allow for additional multiplexing with nucleic acid purification or other markers of cell function.

Presenters:

Dr. Terry Riss started the Cell Biology program at Promega Corporation in 1990 and held several R&D and Project Management positions since. Dr. Riss managed development of cell viability, cytotoxicity, apoptosis, and protease assay systems and also lead efforts to identify and promote multiplexing of cell-based assays to determine the mechanism of cell death. Dr. Riss now serves as Senior Product Manager, Cell Health involved in outreach educational training activities including validating assay systems applied to 3D cell culture models. Dr. Riss also serves as an editor of the In Vitro Cell-Based Assays section of the Assay Guidance Manual hosted by The National Center for Advancing Translational Sciences (NCATS) at the NIH.

Dr. Austin Mogen is a Field Application Scientist at Corning Life Sciences. He received his doctorate from the University of Florida and gained industry experience as both a Senior Scientist of upstream process development and Supervisor for manufacturing of viral vectors. In this position he focused on bioprocess development, closed system solutions for cell culture scale-up, and production of viral vectors for cell and gene therapy. Dr. Mogen works extensively with academic researchers and process development groups, optimizing cell culture assays and cellular scale-up conditions. In addition, he focuses on collaborations utilizing high-end 3D technology products such as the Corning spheroid microplates, transwell inserts, and extracellular matrices to provide more in vivo models for biological research and therapeutic response.
Recorded Oct 23 2019 70 mins
Your place is confirmed,
we'll send you email reminders
Presented by
Terry Riss, Ph.D. and Austin Mogen, Ph.D.
Presentation preview: Ask the Experts: 3D Cell Culture and Assays

Network with like-minded attendees

  • [[ session.user.profile.displayName ]]
    Add a photo
    • [[ session.user.profile.displayName ]]
    • [[ session.user.profile.jobTitle ]]
    • [[ session.user.profile.companyName ]]
    • [[ userProfileTemplateHelper.getLocation(session.user.profile) ]]
  • [[ card.displayName ]]
    • [[ card.displayName ]]
    • [[ card.jobTitle ]]
    • [[ card.companyName ]]
    • [[ userProfileTemplateHelper.getLocation(card) ]]
  • Channel
  • Channel profile
  • オルガノイド培養とハイスループット化の試み Dec 17 2019 6:00 am UTC 60 mins
    コーニングインターナショナル株式会社 ライフサイエンス事業部 江藤哉子
    スフェロイドとオルガノイドの違いに始まり、オルガノイドの活用や2020年1月発売予定の新製品、マトリゲル基底膜マトリックス オルガノイド形成用を使用した、3D気道オルガノイドのハイスループット遺伝子発現解析についてお話します。

    <セミナーのポイント>
    オルガノイドとスフェロイドとの違いやオルガノイドの特長について、実例や画像を交えながらご紹介します。
  • Ask a Corning Scientist: 3D Cell Culture and Bioprocess Recorded: Dec 5 2019 31 mins
    Audrey Bergeron, Applications Scientist & Ann E. Rossi, Ph.D, Senior Bioprocess Applications Scientist, Corning Life Sciences
    Advanced cell culture techniques and scale-up offer exciting research opportunities; however, they also come with complex challenges and questions. Corning Life Sciences knows cells – you might even say it’s in our DNA with over 100 years of experience in the field.

    In this webinar, Corning scientists put their expertise in the areas of 3D cell culture and bioprocess to work as they answered submitted questions from the audience.

    Webinar highlights:
    •Our lab is new to working with spheroids. What are some options for spheroid formation? (2:02 - 4:10)
    •How do you perform media changes in the 96-well Corning spheroid microplate without losing spheroids? (4:11 - 5:49)
    •How do you dissociate single cells from a spheroid for analysis? (5:50 - 6:51)
    •How do you transfer spheroids? (6:54 - 7:30)
    •What is the surface area of the Corning® CellSTACK® 10-layer vessels? (7:56 - 10:02)
    •Is there an easier way to fill Corning CellSTACK 10-layer vessels? (10:03 - 11:33)
    •What is the maximum culture volume for the 5L expansion bag? (11:34 - 13:25)
    •What cell types can I culture in the 5L Erlenmeyer flask? (13:26 - 15:25)

    Live Q&A session with audience (16:10 - 29:30)
    •How do you prevent organoids from sticking to collection tubes?
    •How do I check the cell confluence in a HYPERFlask?
    •And many more questions
  • Special Joint Webinar on Three-dimensional Models and Retinal Organoids Recorded: Dec 5 2019 50 mins
    Franziska Wienholz and Philip Wagstaff
    Three-dimensional Models: Organoid Study and Co-culture for Oncology
    Three-dimensional (3D) cell culture environments provide structural and biochemical clues for cellular differentiation and functionality. For specialized cell types such as primary cells and stem cells, a two-dimensional (2D) growth substrate may not be sufficient to support complex cellular behaviors such as cell polarity, morphology, spheroid formation, signal transduction, and tissue-specific gene expression. This seminar will introduce the newest tools developed to help researchers: Corning® Matrigel® matrix for organoid culture and Corning Elplasia® plates for bulk production of spheroids. We will also present advanced case studies using spheroids and organoids.

    Presenter:
    Franziska Wienholz is a Scientific Support Specialist at Corning Life Sciences.

    Retinal Organoids: Developing Disease Models to Better Understand Glaucoma Pathogenesis
    Glaucoma refers to a group of complex genetic diseases that affect the retinal ganglion cells that relay visual input along the optic nerve to the visual cortex. In glaucoma, these cells are damaged and degenerate, resulting in permanent progressive visual field loss and ultimately, blindness. Retinal organoids are routinely used to model glaucoma in vitro; however, a drawback is the lengthy culture times to generate retinal ganglion cells, which can be 2 months or longer. This can be reduced by using a 3D Corning Matrigel matrix culture, which has been used to generate retinal ganglion cells in just 28 days. With advances in gene editing, retinal organoids can be used to model genetic mutations that lead to the development of glaucoma and provide clues to future therapies to halt the loss of vision.

    Presenter:
    Philip Wagstaff is currently a Ph.D. researcher studying the genetics of glaucoma at University of Amsterdam, Netherlands.
  • バイオ医薬研究・開発・製造における技術導入ポイントとアプリケーションの紹介 Recorded: Nov 19 2019 61 mins
    コーニングインターナショナル株式会社  ライサイエンス事業部 石渡 孝至
    ベクター生産や再生医療用細胞の産業応用への期待が高まるにつれ、生存細胞を最大効率で回収することが求められています。そこでシングルユース製品の攪拌システム(旋回・振とう・上下対流)について細胞培養事例を含めて概説します。

    ポイント:
    ・大量培養容器とクローズドシステムソリューション
    ・ポリスチレン製マイクロキャリア法の紹介
    ・品質管理体制 (原料樹脂・溶出物・リーク・滅菌など)
    ・最新の培養事例紹介 (Vero・hMSC・HEK293Tなど)
  • Ask the Experts: 3D Cell Culture and Assays Recorded: Oct 23 2019 70 mins
    Terry Riss, Ph.D. and Austin Mogen, Ph.D.
    3D culture experts from Corning and Promega answer questions about common methods to form 3D structures, and factors to consider when evaluating cell-based assays for use with 3D structures. Learn about the novel viability and cytotoxicity assays that do not lyse cells and allow for additional multiplexing with nucleic acid purification or other markers of cell function.

    Presenters:

    Dr. Terry Riss started the Cell Biology program at Promega Corporation in 1990 and held several R&D and Project Management positions since. Dr. Riss managed development of cell viability, cytotoxicity, apoptosis, and protease assay systems and also lead efforts to identify and promote multiplexing of cell-based assays to determine the mechanism of cell death. Dr. Riss now serves as Senior Product Manager, Cell Health involved in outreach educational training activities including validating assay systems applied to 3D cell culture models. Dr. Riss also serves as an editor of the In Vitro Cell-Based Assays section of the Assay Guidance Manual hosted by The National Center for Advancing Translational Sciences (NCATS) at the NIH.

    Dr. Austin Mogen is a Field Application Scientist at Corning Life Sciences. He received his doctorate from the University of Florida and gained industry experience as both a Senior Scientist of upstream process development and Supervisor for manufacturing of viral vectors. In this position he focused on bioprocess development, closed system solutions for cell culture scale-up, and production of viral vectors for cell and gene therapy. Dr. Mogen works extensively with academic researchers and process development groups, optimizing cell culture assays and cellular scale-up conditions. In addition, he focuses on collaborations utilizing high-end 3D technology products such as the Corning spheroid microplates, transwell inserts, and extracellular matrices to provide more in vivo models for biological research and therapeutic response.
  • Cerebral Organoids as a 3D Model for Glioma Recorded: Sep 26 2019 50 mins
    Amanda Linkous, Ph.D.
    The prognosis of patients with glioblastoma (GBM) remains dismal with a median survival of approximately 15 months. Current preclinical GBM models are limited by the lack of a “normal” human microenvironment and the inability of many tumor cell lines to accurately reproduce GBM biology. To address these limitations, our guest presenter and her team have established a unique model system whereby they can retro-engineer patient-specific GBMs using patient-derived glioma stem cells (GSCs) and human embryonic stem cell (hESC)-derived cerebral organoids. Their cerebral organoid glioma (GLICO) model shows that GSCs home toward the human cerebral organoid, and deeply invade and proliferate within the host tissue forming tumors that closely phenocopy patient GBMs. Furthermore, cerebral organoid tumors form rapidly and are supported by an interconnected network of tumor microtubes that aids in the invasion of normal host tissue. This GLICO model provides a new system for modeling primary human GBM ex vivo and for high throughput drug screening.


    Guest Presenter Bio:
    Dr. Amanda Linkous previously served as the Director of the Starr Foundation Cerebral Organoid Translational Core at Weill Cornell Medicine (New York, NY). She completed her postdoctoral training in the Neuro-Oncology Branch at the National Cancer Institute (Bethesda, MD). Dr. Linkous is currently the Scientific Center Manager for the NCI's Center for Systems Biology of Small Cell Lung Cancer (SCLC) at Vanderbilt University, where she is developing similar 3D model systems to study the biology and refractory nature of SCLC (Nashville, TN)
  • Corningシングルユース技術による細胞培養アプリケーションセミナー Recorded: Sep 3 2019 62 mins
    コーニングインターナショナル株式会社ライフサイエンス事業部 石渡孝至
    製品プロトコールを基に細胞培養事例の紹介と周辺装置を含めたプロセスソリューションについて概説します。
  • Cell Culture Fundamentals Recorded: Aug 15 2019 44 mins
    Kyung-A (Katie) Song, Ph.D.
    Cell culture is a fundamental technique for a variety of applications. Cells are cultured in controlled conditions including temperature, gas exchange, pH, and culture media (energy and nutrients). This webinar will introduce basic concepts of cell culture and provide tips on how to succeed in culturing mammalian cells.

    In this webinar, we will review:
    •Introduction to cell culture
    •Critical factors for successful cell culture
    •Cell culture tips and techniques

    Presenter Bio:

    Dr. Kyung-A Song graduated from Sungkyunkwan University with a Ph.D. in molecular biology and cancer immunology. She completed her postdoctoral fellowship in pharmaceutical research and drug discovery at Virginia Commonwealth University. She has extensive experience in a variety of cell culture techniques including isolation and culture of primary patient cells. She has worked with xenograft models and many cancer cell lines, and she has expertise in in vitro cell based functional assays and in vivo experiments. She is currently a Scientific Support Specialist for Corning Life Sciences where she answers customer inquiries about all products in the extensive Corning Life Sciences product portfolio.
  • Advanced Models for 3D Cancer Applications Recorded: May 22 2019 23 mins
    Audrey Bergeron, Applications Scientist
    In recent years, three-dimensional (3D) cell culture models have been emerging as the preferred in vitro cancer model as they better mimic the in vivo tumor microenvironment. For instance, 3D cultures develop hypoxic cores and demonstrate gradients of soluble factors and a diffusion profile for drugs similar to solid tumors. Understanding the interactions between cancer cells and other cell types in the tumor microenvironment is also critical to predicting therapeutic efficacy.

    In this webinar, Audrey Bergeron will review:
    •An introduction to 3D cell culture
    •Assay techniques in spheroid microplates
    •Several 3D cancer models and technology tools used to better capture the complexity of the tumor microenvironment in assay-compatible formats.

    About the Presenter:
    Audrey Bergeron is an applications scientist for Corning Life Sciences. She evaluates new products and develops protocols and technical documents using Corning cell culture products. She also provides product training to Corning employees and customers, and assists technical services with customer support by helping to troubleshoot customer experiments.
  • Corning®バイオプロセスの最新培養技術とシングルユース技術導入ポイントセミナー Recorded: Mar 13 2019 60 mins
    コーニングインターナショナル株式会社  ライサイエンス事業部 石渡 孝至
    バイオロジクスの大量培養を具現化に向け、幹細胞、癌細胞、タンパク発現系細胞の基礎的培養技術を示しながら、弊社のシングルユース品質規格(ISO / GMP / QMS)について概説します。

    <セミナーのポイント>
    細胞治療・遺伝子治療を中心にバイオプロセス開発が急速に進められております。幹細胞、癌細胞や標的タンパクを発現する細胞による再生医療・遺伝子治療を具現化する際に、最も大きな懸案事項となりますのが質、量そしてコストの問題があります。 そこでコーニングは、安全、安価かつ簡便な最新シングルユース技術についてレギュレーション、導入事例によるマーケットトレンドを示しながら、セルカルチャー(可溶性マイクロキャリア、撹拌・振盪容器、多段型培養容器)、閉鎖系チュービング(プレアッセンブル、無菌接続)、パッケージング(回収 / セルバンク)、データインテグリティ(バーコードによるトレーサビリティ管理)、品質規格(成型施設環境、BSE/TSE、発熱物質(Pyrogen)、USPクラスⅥ原料樹脂規格 / 滅菌保証レベル / エンドトキシン規格 / QC試験)および品質に関するドキュメンテーションサポート(バリデーションガイド)に関する情報を概説します。
  • Cell Culture Masterclass: A 10-Point Plan to Prevent Contamination Recorded: Jan 28 2019 44 mins
    Franziska Wienholz, Scientific Support Specialist, Corning Life Sciences
    Cell Culture Masterclass: A 10-Point Plan to Prevent Contamination

    All cell culture laboratories have experienced it: cell culture contamination and its inevitable loss of material. Contaminants may be biological or chemical, visible or invisible, destructive or seemingly benign, but in all cases, they adversely affect both the use of your cell cultures and the quality of your research.

    Identification and, even better, prevention of contamination are crucial for ensuring high-quality research. This expert webinar will present an overview on how to prevent, recognize and tackle contamination by introducing good lab practices and targeted interventions.

    If you work with cell culture daily or are interested in setting up prevention strategies to minimize contamination, then join SelectScience and Franziska Wienholz, scientific support specialist of Corning Life Sciences, for this educational masterclass. Franziska will share a 10-point plan to prevent contamination and provide a comprehensive refresher on all aspects of cell culture.

    Key learning objectives:

    Understand the basic rules of cell culture
    Know how to properly manage cell lines to prevent cross-contamination
    Be able to implement a 10-point plan to contamination prevention in your laboratory

    Who should attend:
    Scientists working with cell culture on a daily basis
    Cell culture managers who are interested in setting up programs to prevent contamination and determine authentication of cells
    Researchers that struggle with contamination issues
  • Advances in three-dimensional Cell Culture Recorded: Jan 28 2019 62 mins
    Dr. Richard E. Eglen, Vice President and General Manager, Corning Life Sciences.
    Advances in three-dimensional Cell Culture in Drug Research, Discovery and Biologic Manufacture.

    Cellular research is optimal when using physiologically relevant cell phenotypes and genotypes of human origin. This assertion has accelerated the adoption of primary cells, stem cells, and, increasingly, patient-specific cells in drug discovery. Over the past five years, technological improvements in 3D cell culture technology, to better mimic in vivo physiology, have also advanced. This is true not only in the areas of cancer and neurological research, but also in the assessment of clinical candidates for metabolic and toxicological liabilities. Furthermore, 3D cell culture is facilitating novel approaches to both the scale-up and manufacture of biologics, including those used in immuno- and stem cell-based therapies.

    Are you a scientist interested in 3D cell culture? Join this expert webinar, in which Dr. Richard Eglen will discuss the importance of 3D cell culture in drug discovery and manufacture and how technological improvements are causing significant advancements in this field.

    Learn about:

    - The existing and future impacts of 3D cell culture technology on fundamental research
    - The impacts of 3D cell culture on drug discovery and manufacture, particularly in the context of using phenotypically relevant cells?
    - The potential for spheroids, organoids, scaffolds, and hydrogels in cellular research and compound identification, screening, and development?
  • 3Dスフェロイド培養のアプリケーションとミニチュアライズ Recorded: Dec 20 2018 54 mins
    コーニングインターナショナル株式会社 ライフサイエンス事業部 江藤 哉子
    弊社独自の超低接着表面・形状のプレートを用いた三次元がんスフェロイドや、免疫細胞の作用モデルなどのハイスループットスクリーニング、オルガノイド作製の実例を交えて概説いたします。

    今回は、3Dスフェロイドを使用したアプリケーションをご検討中のみなさまに向けて、基礎から実際のアプリケーションまでをお話しいたします。
  • 多段型セルカルチャーおよびマイクロキャリアによる細胞培養技術セミナー Recorded: Nov 21 2018 61 mins
    コーニングインターナショナル株式会社  ライサイエンス事業部 石渡 孝至
    バイオロジクスの治験薬製造における効果的な細胞培養と分離工程の簡素化を実現させる幹細胞・癌細胞の基礎的培養技術を概説いたします。
    今回は、大量培養に向けたシングルユース技術導入のポイントを概説します。

    <セミナーのポイント>
    実際に静置型/撹拌型のシングルユース培養容器を使用した時のヒト間葉系幹細胞(hMSC)及びヒト胎児腎細胞(HEK293T)の培養データを例示します。
  • Short Tutorial: Techniques for Harvesting Adherent Cells Recorded: Jun 18 2018 5 mins
    Corning Life Sciences
    Harvesting your cells is an important step in maintaining healthy cultures. Good technique will lead to a healthy single cell suspension. There are many harvesting options depending on the cell line and application. In this video, we will discuss some tips/tricks to harvesting cells.
  • Short Tutorial: Corning® Matrigel® Matrix: Thin Coating Method Best Practices Recorded: Jun 4 2018 3 mins
    Corning Life Sciences
    Looking for tips using Matrigel matrix before you coat and plate your cells? We’ve got you covered. Watch our short tutorial for best practices on how to coat Matrigel Matrix as a thin gel layer.
    In search of more Matrigel matrix user information? We’ve summarized some other great tips and tricks for Matrigel in our newly published "Ultimate Guide to Matrigel Matrix" – download it today: https://goo.gl/9Nq5Td
  • Short Tutorial: Corning® Matrigel® Matrix: Thin, Non-gelled Protein Layer Method Recorded: Jun 4 2018 3 mins
    Corning Life Sciences
    Trying to use Matrigel matrix for cell attachment? Watch our short video to learn the best way to coat Matrigel matrix in a thin, non-gelled protein layer.
    Interested in some more Matrigel matrix best practices? We’ve summarized some other great tips and tricks for Matrigel in our newly published "Ultimate Guide to Matrigel Matrix" – download it today: https://goo.gl/9Nq5Td
  • Short Tutorial: Corning® Matrigel® Matrix: Thick Coat Method Best Practices Recorded: Jun 4 2018 3 mins
    Corning Life Sciences
    Did you know that cells can be cultured inside Matrigel matrix? Learn more about the “thick-coating” method to embed cells in a 3D, more in vivo-like environment.
    Looking for more great Matrigel Matrix tips and tricks? We’ve summarized a bunch in our newly published "Ultimate Guide to Matrigel Matrix" – download it today: https://goo.gl/9Nq5Td
  • Short Tutorial: Corning® Matrigel® Matrix: Thawing Best Practices Recorded: Jun 1 2018 3 mins
    Corning Life Sciences
    We get a lot of questions on the best ways to thaw Matrigel matrix. Are the color changes normal? What are the best ways to ensure homogeneity? Get the answers to these questions and other best practices for effectively thawing the most widely used ECM in research, Corning Matrigel Matrix. We’ve summarized some other great tips and tricks for Matrigel in our newly published "Ultimate Guide to Matrigel Matrix" – download it today: https://goo.gl/9Nq5Td
  • 最新技術 Corning®可溶性マイクロキャリアの培養システムを初公開 Recorded: May 29 2018 30 mins
    コーニングインターナショナル株式会社 ライフサイエンス事業部 石渡孝至
    シングルユース技術導入ポイントとなる効果的な細胞培養と分離工程の簡素化を実現するアプリケーションをMSC(間葉系幹細胞)を例に概説します。
Training for Life Science Researchers
The Corning Scientific Seminar Series is a series of free, online technical presentations that provide novel tips, best practices and proven techniques to help advance your research. Delivered by scientists to scientists, these one-hour broadcasts offer useful information and tips for lab technicians and researchers.

Embed in website or blog

Successfully added emails: 0
Remove all
  • Title: Ask the Experts: 3D Cell Culture and Assays
  • Live at: Oct 23 2019 7:35 pm
  • Presented by: Terry Riss, Ph.D. and Austin Mogen, Ph.D.
  • From:
Your email has been sent.
or close