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Ask a Corning Scientist: 3D Cell Culture and Bioprocess

Advanced cell culture techniques and scale-up offer exciting research opportunities; however, they also come with complex challenges and questions. Corning Life Sciences knows cells – you might even say it’s in our DNA with over 100 years of experience in the field.

In this pre-recorded webinar, Corning scientists put their expertise in the areas of 3D cell culture and bioprocess to work as they answered some of the commonly asked questions they receive.

Webinar highlights:
•Our lab is new to working with spheroids. What are some options for spheroid formation? (2:02 - 4:10)
•How do you perform media changes in the 96-well Corning spheroid microplate without losing spheroids? (4:11 - 5:49)
•How do you dissociate single cells from a spheroid for analysis? (5:50 - 6:51)
•How do you transfer spheroids? (6:54 - 7:30)
•What is the surface area of the Corning® CellSTACK® 10-layer vessels? (7:56 - 10:02)
•Is there an easier way to fill Corning CellSTACK 10-layer vessels? (10:03 - 11:33)
•What is the maximum culture volume for the 5L expansion bag? (11:34 - 13:25)
•What cell types can I culture in the 5L Erlenmeyer flask? (13:26 - 15:25)

Live Q&A session with audience (16:10 - 29:30)
•How do you prevent organoids from sticking to collection tubes?
•How do I check the cell confluence in a HYPERFlask?
•And many more questions

If you have a question about 3D cell culture or bioprocess while watching the webinar live, we encourage you to submit it using the questions on the viewing screen and a member of our scientific support team will follow-up with you via email.
Recorded Apr 29 2020 30 mins
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Presented by
Audrey Bergeron, Applications Scientist & Ann E. Rossi, Ph.D, Senior Bioprocess Applications Scientist, Corning Life Sciences
Presentation preview: Ask a Corning Scientist: 3D Cell Culture and Bioprocess

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  • AJINOMOTO & Corning 共同セミナー Nov 18 2021 6:00 am UTC 60 mins
    コーニング 江藤 哉子 / 味の素 原田 英里
    演題1
    間葉系幹細胞の培養に適した培養表面と使用例
    間葉系幹細胞の培養では様々な培養表面が用いられています。コーニングが提供する培養表面には、プレートの素材であるポリスチレンに酸素導入により官能基を付与したCorning® CellBIND® surfaceや、幹細胞の培養に用いられるビトロネクチンの部分合成ペプチドであるCorning Synthemax® -II などがあります。今回はその特長や使用例をご紹介します。

    演題2
    臨床研究に向けた間葉系幹細胞大量培養の最適化を目指して
    StemFit For Mesenchymal Stem Cell(MSC)は、高い性能、安定性、安全性を兼ね備えた間葉系幹細胞(MSC)用の拡大培養培地です。培地組成中に、ヒト由来成分(血清成分、血小板溶解物等)を含まないため、ロット間の性能差が少なく、安定した細胞培養が可能です。MSCの培養において、本培地は従来の血清添加培地に比べ、高い増殖性能を発揮します。また、PMDAの再生医療等製品材料適格性確認書を取得しています。このStemFit For MSCとコーニングの CellBIND 表面やCorning Synthemax -II との組み合わせにより、組織からの効率的なMSC単離や臨床研究に必要な量への細胞拡大培養が実現可能です。本ウェビナーでは、その事例をご紹介いたします。


    ※本セミナーお申込みの際に入力いただきました個人情報は、コーニングインターナショナル株式会社と味の素株式会社とで共有させていただきます。またその情報は、質問の回答およびCORNING NEWS などのお知らせメールの配信を含む弊社および味の素株式会社の営業・マーケテイング活動に使用させていただきます。
    【コーニングのプライバシーポリシー】https://www.corning.com/jp/jp/about_us/japan_privacy_policy.aspx
    【味の素のプライバシーポリシー】https://www.ajinomoto.co.jp/privacy/
  • Choosing the Right Cell Culture Surface: Modified Surfaces Oct 28 2021 4:00 pm UTC 60 mins
    Ann Rossi Bilodeau, PhD, Corning Life Sciences
    Choosing the right cell culture surface can be a challenge, especially with a diverse range of options extending beyond traditional tissue culture-treated polystyrene. Ultimately, the cell culture surface mimics cells’ native environments and drives cell morphology, phenotype, and function. Consequently, the choice of cultureware is critical for successful cell growth.

    In this webinar you will learn:
    • A brief history of the evolution of cell culture surfaces
    • The difference between surface modifications and coating
    • Basic principles behind tissue culture treatment
    • When to use enhanced attachment surfaces
  • 3D Cell Culture Techniques; For Spheroid and Organoid Applications Oct 28 2021 5:00 am UTC 60 mins
    Meng-Tsz Tsai
    3D cell culture is gaining traction in basic research and medicine discovery due to the more physiologically relevant characteristics than traditional (2D) cell culture. Corning has delivered pioneer tools that have advanced the science of 3D cell culture within the past decades. In this webinar we will introduce 3D cell culture techniques, applications, and culture tips and tricks that will help you to get started on the path to 3D cell culture.
  • Understanding Cell Culture Media Recorded: Oct 21 2021 40 mins
    Sherwin (Xiaoyu) Zhu, PhD
    This webinar will introduce the primary characteristics of basal media and will provide tips and best practices to select correct basal media and optimize cell culture. In addition, you will learn:

    • Considerations to select correct media formulation
    • Key factors that affect media performance

    Dr. Xiaoyu Zhu is a Scientific Support Specialist for North America at Corning Life Sciences. He received a Ph.D. in Cell Biology at Beijing Normal University. Dr. Zhu was an Associate Professor at the Chinese Center for Disease Control and Prevention (China CDC). In his current role, he supports customers with product selection, application support, and troubleshooting. He has been with Corning for eight years.
  • Biomaster & Corning 共同セミナー Recorded: Oct 19 2021 61 mins
    コーニングインターナショナル 石渡 孝至 / バイオマスター 吉田 進也
    演題1:
    幹細胞を用いた多段型培養容器システムと応用
    CPC内で無菌的に細胞を大量培養するにあたり、特別な設備投資なくスケールアップの予測が容易な培養システムの最新動向を幹細胞開発研究を事例に概説します。

    演題2:
    細胞濃縮洗浄システムの紹介
    細胞濃縮洗浄システムは、細胞懸濁液の濃縮、洗浄を自動的に行うシステムで、培養した間葉系幹細胞、iPS細胞、リンパ球などの濃縮洗浄に用いることができ、例えば細胞懸濁液3Lを約30分で濃縮洗浄できます。本セミナーでは、システムの特徴である「自動化処理による工程の標準化」「閉鎖系回路によるコンタミリスクの低減」「凝集過程を経ずにシングルセルのままで濃縮洗浄」をご紹介します。

    ※本セミナーお申込みの際に入力いただきました個人情報は、コーニングインターナショナル株式会社と株式会社バイオマスターとで共有させていただきます。またその情報は、質問の回答およびCORNING NEWS などのお知らせメールの配信を含む弊社の営業・マーケテイング活動に使用させていただきます。
    【コーニングのプライバシーポリシー】https://www.corning.com/jp/jp/about_us/japan_privacy_policy.aspx
  • Characterization and Diagnostic Applications of Patient-derived Tumor Organoids Recorded: Oct 18 2021 22 mins
    Florencia Madorsky Rowdo, Ph.D., Postdoctoral Associate at Englander Institute for Precision Medicine
    Title: Characterization and Diagnostic Applications of Patient-derived Tumor Organoids

    3D CELL CULTURE SUMMIT MASTER SERIES

    As part of the Englander Institute for Precision Medicine, the Tumor Organoid Platform is an active program focused on development of 3D patient derived tumor organoids (PDTO) from metastatic and primary anatomic sites, obtained through biopsies, surgical resections and rapid autopsy procedures. In this setting, the Tumor Organoid Platform has established a “living biobank” of 160 patient tumor organoids comprising 14 different tumor types. These models are used for High Throughput Drug Screening, the identification of new targets using CRISPR Cas9 Screenings and the generation of co-cultures with different cell types present in the tumor microenvironment, including immune cells and fibroblasts.
  • Cultivo celular en 3D Recorded: Oct 14 2021 58 mins
    Gisela Pennacchio, pHD
    Principales Aspectos del Cultivo 3D
    Ventajas y Herramientas para su Uso
    Tecnicas de Generacion de Esferoides
    Caracterizacion de Esferoides
  • Male Sex Hormones Increase Excitatory Neuron Production in HCO Recorded: Oct 12 2021 26 mins
    Iva Kelava, Ph.D., Postdoctoral Fellow, MRC Laboratory of Molecular Biology
    Title: Male Sex Hormones Increase Excitatory Neuron Production in Human Cerebral Organoids

    3D CELL CULTURE SUMMIT MASTER SERIES

    Bioengineered tissue platforms offer a new paradigm for modeling human pathophysiology and testing drug efficacy and safety. To model whole-body physiology and systemic diseases, we developed the biomimetic InterOrgan platform in which matured human tissues are connected in a way that allows maintaining distinct functionalities of different organs. Each tissue is cultured in its own optimized environment and integrated via recirculating vascular flow containing monocytes, by a matured and selectively permeable endothelial barrier. Tissues linked by vascular perfusion maintained their molecular, structural, and functional phenotypes over four weeks of culture, recapitulate multi-organ toxicity of doxorubicin and clinically relevant miRNA biomarkers, and show utility for immune-oncology applications.
  • Upstream Viral Vector Manufacturing: Transfections of Adherent Cultures at Scale Recorded: Oct 7 2021 44 mins
    Cassie-Marie Peigné, PhD, Polyplus-transfection® and Ann Rossi Bilodeau, PhD, Corning Life Sciences
    Many cell and gene therapy applications are dependent on viral vectors for recombinant gene delivery. Efficient transfection of a large biomass of cells represents a significant challenge for transient viral vector production systems. However, identifying a scalable adherent cell culture platform for high density cell culture and relying on a transfection reagent that can ensure robust and reproducible viral yields at scale are essential to streamline transition from process development to manufacturing.

    Introduction: 1:36 – 7:57
    – Viral vector use in cell and gene therapy applications
    – Challenges for vector production
    Considerations for Choosing a Cell Culture Platform: 7:58 – 14:43
    – Adherent versus suspension
    – Typical upstream scale-up/scale-out
    Bottlenecks to Scalable Transfections: 14:44 – 30:40
    – Science behind Polyplus® reagents, featuring PEIpro®
    – Protocol review for Corning® CellSTACK® and CellCube® vessels
    Q&A Session: 30:41 – 43:09
  • Engineering Integrated Multi-Tissue Platforms for Modeling Human Pathologies Recorded: Sep 20 2021 30 mins
    Kacey Ronaldson Bouchard, Ph.D., Associate Research Scientist, Columbia University
    Title: Engineering Integrated Multi-Tissue Platforms for Modeling Systemic Human Pathologies

    3D CELL CULTURE SUMMIT MASTER SERIES

    Bioengineered tissue platforms offer a new paradigm for modeling human pathophysiology and testing drug efficacy and safety. To model whole-body physiology and systemic diseases, we developed the biomimetic InterOrgan platform in which matured human tissues are connected in a way that allows maintaining distinct functionalities of different organs. Each tissue is cultured in its own optimized environment and integrated via recirculating vascular flow containing monocytes, by a matured and selectively permeable endothelial barrier. Tissues linked by vascular perfusion maintained their molecular, structural, and functional phenotypes over four weeks of culture, recapitulate multi-organ toxicity of doxorubicin and clinically relevant miRNA biomarkers, and show utility for immune-oncology applications.
  • Yamaha Motor & Corning 共同セミナー Recorded: Sep 15 2021 61 mins
    コーニング 江藤 哉子 / ヤマハ発動機 原田 額郎 Ph.D.
    演題1
    コーニングが提供する3D培養製品-超低接着表面とゲル基質
    オルガノイドやスフェロイドを形成するために広く用いられる、コーニングの超低接着表面及びCorning® マトリゲル基底膜マトリックスとその培養手法をご紹介いたします。

    演題2
    細胞ピッキング&イメージングシステムCELL HANDLER™ の基本機能と活用例
    CELL HANDLERは、シングルセルから3D細胞まで幅広い細胞を対象としたイメージベースの細胞ピッキング装置です。独自のピッキング技術は、ダメージレスに目的細胞を分取し、人手では困難であったゲル包埋オルガノイドや2D接着細胞の単離も実現します。これにより、実験効率や再現性、トレーサビリティを向上し、幹細胞研究やがん研究分野の発展に貢献します。

    ※本セミナーお申込みの際に入力いただきました個人情報は、コーニングインターナショナル株式会社とヤマハ発動機株式会社(ych@yamaha-motor.co.jp,
    https://www.yamaha-motor.co.jp/hc/)とで共有させていただきます。
    またその情報は、質問の回答およびCORNING NEWS などのお知らせメールの配信を含む弊社の営業・マーケテイング活動に使用させていただきます。
    【コーニングのプライバシーポリシー】https://www.corning.com/jp/jp/about_us/japan_privacy_policy.aspx
    【ヤマハ発動機のプライバシーポリシー】https://global.yamaha-motor.com/jp/privacy/
  • 코닝의 3D spheroid와 organoid 배양 관련 제품과 사용 예시 Recorded: Aug 13 2021 53 mins
    Seo Kyubin (Andrew)
    3D 세포 배양에 대한 연구는 여전히 활발하게 진행되고 있으며, 이에 대한 관심과 수요가 지속적으로 증가하고 있는 상황입니다.
    많은 3D 세포 배양 방법 중에서도 스페로이드 & 오가노이드 배양과 관련된 코닝의 제품을 소개하고, 관련 예와 실험적인 팁을 설명합니다.
    Airway 오가노이드에 대한 배양법과 관련 제품들을 소개하며, COVID-19 virus에 대한 오가노이드 model로 어떻게 사용될 수 있는지 설명합니다.
  • Olympus & Corning共同セミナー Recorded: Jul 20 2021 46 mins
    コーニングインターナショナル株式会社 石渡 孝至 / オリンパス株式会社 横町 祐樹
    演題1
    コーニングバイオプロセス多層型培養システムCellSTACK、HYPERStack容器の導入事例紹介
    近年、バイオロジクス分野におけるベクター産生や再生医療用細胞の産業応用で期待が高まり大量培養が求められています。本セミナーでは大量細胞培養におけるクローズドシステムによる無菌操作法と細胞培養事例を概説いたします。

    演題2
    細胞培養スケールアッププロセス開発の品質管理・省力化に貢献するCM20
    インキュベーションモニタリングシステム CM20は、インキュベーター内で培養中の細胞数や密度を自動で計測します。培養従事者がクリーンルームに入ることなく培養状況を確認でき、これまで計測に費やしていた時間やコストが大幅に削減されます。大型容器や多層フラスコまでのスケールアッププロセスにおいても一貫したデータ計測が可能なため、スケールアップのプロセス開発にも大いに貢献します。

    ※本セミナーお申込みの際に入力いただきました個人情報は、コーニングインターナショナル株式会社とオリンパス株式会社とで共有させていただきます。またその情報は、質問の回答およびCORNING NEWS などのお知らせメールの配信を含む弊社の営業・マーケテイング活動に使用させていただきます。
    【コーニングのプライバシーポリシー】https://www.corning.com/jp/jp/about_us/japan_privacy_policy.aspx
    【オリンパスのプライバシーポリシー】https://www.olympus.co.jp/products/policy/privacy_management/policy_jp.html
  • Challenges and Processes for Development and Scale-up of Stem Cell Therapies Recorded: Jul 16 2021 62 mins
    Carolyn Wrightson, Ph.D., VetStem Biopharma and Austin Mogen, Ph.D., Corning Incorporated
    GMP cell manufacturing for new clinical cell therapies is a rapidly expanding need in the life science space. This presentation will discuss the foundation of cell therapy product development considerations when preparing to launch into manufacture of materials in support of early Phase clinical trials to commercialization. Historical development of an autologous canine mesenchymal stem-cell product that facilitated the ability to rapidly respond to develop and manufacture an allogeneic human MSC product for treatment of COVID-19 patients with ARDS will be presented.
    Learning Objectives:
    - MSC isolation from tissue to success in primary cell culture
    - Characterization of cell lines
    - Avoiding development pitfalls in the scale-up of cell therapy manufacturing

    Speaker:
    Carolyn Wrightson, Ph.D.
    Chief of Operations
    VetStem Biopharma

    Austin Mogen, Ph.D.
    Senior Field Application Scientist
    Corning Incorporated | Life Science
  • Corning® CellBIND: Reducing Serum Levels and Culture Costs (Vietnamese Version) Recorded: Jul 13 2021 35 mins
    Dzung Do Van
    A major cost component of cell culture is fetal bovine serum (FBS), an often essential and very expensive. Many researchers routinely use media containing 10 to 20% FBS for growing the cells, but these levels may be higher than cells require. By reducing FBS levels by 50%, researchers may be able to save a considerable amount of money. Not all cell lines will do well at lower FBS levels although many will. This short presentation will help you better understand the steps you can take to reduce your cultures’ FBS levels and save money while keeping your cultures happy.
  • コーニングの3D細胞培養関連製品と使用例 Recorded: Jun 29 2021 53 mins
    コーニングインターナショナル株式会社 ライフサイエンス事業部 江藤 哉子
    ますます需要の高まる3D細胞培養について、その概要とコーニングの各種関連製品を、使用例を含めながらご説明します。また6月1日発売の新製品Corning® 3D組織透明化試薬もご紹介します。

    ※下記URLのコーニングのプライバシーポリシーにご同意のうえご登録ください。ご登録いただいたお名前、ご所属、電子メールアドレス、電話番号、その他の情報は、質問の回答およびCorning Newsなどのお知らせメールの配信を含む弊社の営業・マーケテイング活動に使用させていただきます。
    【プライバシーポリシー】https://www.corning.com/jp/jp/about_us/japan_privacy_policy.aspx
  • 3D Human Liver Spheroids: Moving Towards Meaningful In Vitro Assays Recorded: Jun 9 2021 39 mins
    Michael Johnson, Ph.D., CEO, Visikol, Inc. and Feng Li, Ph.D., Development Associate Corning Life Sciences
    Human liver spheroids are being utilized as a novel in vitro tool for drug safety assessment and liver disease modeling. Corning® HepGo™ Assay-ready 3D Liver Spheroid Kit featuring pre-qualified primary human hepatocytes has been developed to provide researchers an easy access to the more physiologically relevant tool. In this tutorial, we will share our experiences to facilitate a conversation on how to use liver spheroids for in vitro assays.
  • Disease Modeling Using ALI Tools and Systems Recorded: Jun 4 2021 12 mins
    Roxana Ghadessy, Ph.D. and Shabana Islam, Ph.D.- Corning Life Sciences
    From asthma to COPD, ALI models have been critical in researching respiratory diseases. With the recent outbreak and severity of the SARS-CoV-2 virus, this technology is becoming even more critical in the race to understand and treat disease.

    With the popularity of these more complex models growing, join this Teach Me in 10 session to hear Corning Life Sciences Digital Manager Roxana Ghadessy and Corning Life Sciences Product Line Manager Shabana Islam talk to us about disease modeling using ALI tools and systems.
  • Corning Solutions for Cell Therapy Bioprocessing Recorded: May 27 2021 48 mins
    Franco So
    This webinar illustrates the Corning cell isolation and expansion platforms to meet scale-up and other challenges in cell therapy bioprocessing.
    Corning’s recent investments into providing automation around Stackable Vessels operations as well as in optical based cell isolation technologies would decrease lot-to-lot variability for manufacturing.
    For larger-scale applications, Corning’s focus into microcarrier-based technologies accelerates allogenic large-scale production of stem cells.
    Fixed bed platforms such as CellCUBE can also support adherent-based perfusion cultures which would be applicable for exosome-based therapies.
  • 多細胞系からなるiPS細胞由来ヒト肝臓オルガノイドを用いた脂肪性肝炎モデリング Recorded: May 21 2021 31 mins
    東京医科歯科大学 統合研究機構 先端医歯工学創成研究部門 創生医学コンソーシアム臓器発生・創生ユニット 特任研究員 大内 梨江先生
    本セミナーでは、世界に先駆けて開発した免疫系細胞を含む複数種類の細胞で構成されたiPS細胞由来ヒト肝臓オルガノイドの作製方法について解説します。さらに、本オルガノイドを用いて脂肪性肝炎を試験管内で再現した病態モデルの構築手法及び当モデルの活用例について紹介します。
Training for Life Science Researchers
The Corning Scientific Seminar Series is a series of free, online technical presentations that provide novel tips, best practices and proven techniques to help advance your research. Delivered by scientists to scientists, these one-hour broadcasts offer useful information and tips for lab technicians and researchers.

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  • Title: Ask a Corning Scientist: 3D Cell Culture and Bioprocess
  • Live at: Apr 29 2020 5:00 am
  • Presented by: Audrey Bergeron, Applications Scientist & Ann E. Rossi, Ph.D, Senior Bioprocess Applications Scientist, Corning Life Sciences
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