3D Cell Cultures and Tissue Clearing

Presented by

Ian MacCloud, Laboratory Manager at Visikol Inc. and Dr. Ann Rossi Bilodeau, Corning Life Sciences

About this talk

There are many important considerations for how best to image a 3D cell culture model. Moreover, there are numerous high content imaging systems available. This webinar reviews some of the systems available,best practices when imaging 3D cell culture models, and how to employ tissue clearing for improved imaging. Additionally, the presenters share several case studies in which tissue clearing and high content confocal microscopy are integral to addressing specific research questions: • LED vs. laser illumination • Dipping vs. air objectives • Basic principles of confocal microscopy • Best practices for labeling 3D cell culture models • Wide field vs. confocal imaging • 3D image analysis Webinar Highlights: - Introduction: 00:00 - 3:18 - The State of 3D Cell Culture: 3:19 - 8:19 - Tissue Clearing and Labeling Techniques for 3D-CCM: 8:20 - 17:20 - High Content Imaging Considerations: 17:21 - 28:07 - Workflow and Case Study - 3D Cell Culture Models for Immunoncology: 28:08 -38:14 - Q&A Session: 38:15 - 51:48

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The Corning Scientific Seminar Series is a series of free, online technical presentations that provide novel tips, best practices and proven techniques to help advance your research. Delivered by scientists to scientists, these one-hour broadcasts offer useful information and tips for lab technicians and researchers.