Name: Pipetting Techniques and Avoiding Contamination
Start: 2021-05-04T16:00:00Z
End: 2021-05-04T16:00:36.000Z
Location: BrightTALK
Rating: 4.2

The Corning Scientific Seminar Series is a series of free, online technical presentations that provide novel tips, best practices and proven techniques to help advance your research. Delivered by scientists to scientists, these one-hour broadcasts offer useful information and tips for lab technicians and researchers.

Cell culture contamination can result in loss of time, money, and effort. Learn how to reduce contamination risk to optimize your work!

In this webinar, you'll learn about:
-Major cell culture contaminants
-Biological vs Chemical contaminants
-Sources of contaminants
-How to control cell culture contamination

Understanding and Managing Cell Culture Contamination

Exosomes, extracellular vesicles secreted by cells, have emerged as promising candidates for therapeutic applications due to their ability to carry a variety of biomolecules and participate in intercellular communication. This webinar will delve into the exosome production techniques, including production platforms, isolation methods, and quality control measures. Furthermore, in this webinar we will explore the therapeutic potential of exosomes in various diseases, such as cancer, neurodegenerative disorders, and inflammatory conditions.

Exploring the Frontier of Exosome Science: From Production to Therapy

コーニングが独自に開発したハイパーテクノロジー製品は、培養スペースを効率的に活用することが出来る画期的な大量培養向きの細胞培養容器です。このテクノロジーを採用したハイパーフラスコやハイパースタックセルカルチャー容器のアプリケーションをご紹介します。ハイパーテクノロジーと並びスペースを有効活用できるCorning® CellCube® システムも併せてご紹介します。

コーニングが提供する細胞培養のスケールアップソリューション

In this webinar, you'll learn about:
-The difference between 2D and 3D cell culture
-The value of each method
-When to use one method over the other
-Products available to help streamline and improve 3D workflows

2D versus 3D: Benefits of Moving to 3D Cell Culture

Commercial process technologies for biologics manufacturing are facing multiple challenges because of ongoing market economic pressure, uncertainty in market growth and low productivity manufacturing technologies. To overcome these challenges in novel production processes, HYPER Technology is one of technological advantages available. The next generation High Yield PERformance (HYPER) technology lets you grow large quantities of adherent cells in a compact space. The cell production technology uses a unique ultra-thin gas-permeable film to eliminate the air gap within the vessel. This results in a substantial increase in cell growth surface area compared to traditional vessels of comparable cubic footprint.
Join us as we evaluate the available platforms for commercializing adherent cell culture-based products, we will discuss the science of HYPER Technology and how its enabling process intensification and scale up. We will also go through case studies of use of this platform for protein production, cell and gene therapy, including adenovirus, lentivirus and whole cell as final products.

HYPER Technology: Revolutionizing Adherent Cell Culture

Extracellular matrix (ECM) proteins and synthetic hydrogels help cultured cells grow and function in an in vivo-like environment, so choosing the right product is critical.

In this Teach me in 10, we are joined by Dr. Anette Funfak, Field Application manager at Corning Life Sciences. She reviews different types of biological and synthetic hydrogels, as well as their characteristics, limitations and functional applications for building in vivo-like environments.

Synthetic vs. Biological Hydrogels

On-demand content from SLAS2024:

3D cell culture techniques offer significant benefits to researchers trying to recreate a more physiological environment for their experiments. Over the past decade, 3D cell culture has evolved and now encompasses a variety of techniques and applications. In this session, we will discuss emerging trends in 3D cell culture techniques and review technologies that allow users to create 3D environments in a consistent and efficient manner.

Solutions Spotlight: Emerging Trends in 3D Cell Culture Workflows

Spheroids and organoids have significant advantages over traditional two-dimensional (2D) cell cultures, in large part because of their ability to better mimic the architecture of tissue and organs and the in vivo microenvironment. The realistic cellular arrangement makes it possible to study biological behaviors as cell-cell interactions and cell migration in a physiologically meaningful context. Access to this information has important implications for developing personalized medicines and targeted therapies for a wide range of diseases.

In this GEN webinar, Corning Life Sciences’ Hilary Sherman will discuss the advantages of spheroid and organoid models over traditional 2D models and highlight why they are best option for biomedical research and drug development. During the webinar, you’ll learn about the variety of tools available for working with 3D culture and helpful tips for selecting the right tools for different applications.

Flat to Functional: Tools & Techniques for Working with Spheroids and Organoids

Please join us for a free webinar hosted by Pharma's Almanac and sponsored by Corning Life Sciences, "Designing a Recipe for Stem Cell Therapy Success" on Tuesday, December 05, 2023 at 11:00 A.M. EST. 

We are excited to feature two Field Application Scientists from Corning Life Sciences: Ben Josey, Ph.D., and Robert Padilla, MS. 

This webinar will be moderated by David Alvaro, Ph.D., of Pharma's Almanac and will include a live Q&A portion for participants to ask their own questions. 

We will discuss considerations and strategies for transitioning stem cell therapies from research into a production environment. 

In this free webinar, we will cover:  
• Cell type and end-product goals
• Platform and media selection for cell expansion
• Surface chemistry substrate choices and other cell culture success factors
• Succesfully translating your biology as you scale
• De-risking and optimizing processes
• And other biological and operational considerations

Designing a Recipe for Stem Cell Therapy Success

Cell therapies offer a promising approach to treat severe and rare diseases. However, sustained manufacturing of cell therapy products is at risk due to the high costs associated with manufacturing variability and safety assessments. One significant risk in the manufacturing process is contamination of product, which can contribute to millions of dollars in added cost. In this webinar, we will address how integrating closed system approaches during process development or manufacturing can mitigate contamination risk and associated costs.

This webinar will introduce viewers to Corning’s closed system solutions, with attention to the following topics:

Assembly and customization of closed systems
Closed system process considerations and flexibility in design
Scaling up and out using closed systems
Attend this webinar to learn more about:

Benefits of closed systems
Assessing risk of contamination
Key considerations for closed system design

Closed System Solutions for Cell and Gene Therapy Manufacturing

Vaccines are the most effective medical intervention to reduce death and morbidity caused by infectious diseases. They save millions of lives every year and we realized the power of immunization in Covid-19 pandemic. Development of vaccines is difficult because of the biological complexity of the pathogens and the challenges inherent to development of any vaccine.
Join us as we will go through the market and production dynamics in vaccine industry, we will discuss the Corning platforms enabling vaccine process development and commercialization. We will also go through case study of vaccine development process from concept to commercialization including the challenges of platform selection, process optimizations, regulatory pathway and typical timelines for such products.

Vaccines: Concept to Commercialization

Three-dimensional (3D) cultures such as spheroids and organoids are an important part of the research model market, helping to close the gap between cell cultures and animal models. Both organoids and spheroids have been used to create in vivo-like tissue models of cancer subtypes to study novel therapies and to make models for tissue engineering and regenerative medicine studies. But there are some key differences, with important implications for various applications. The right tool for a project is not always obvious. For spheroids and organoids, knowing where the cultures are similar and where they differ will help scientists select the best resource for their projects the first time around.

In this Learning Lab, Hilary Sherman, a Senior Scientist in the Corning Life Sciences Applications Lab, and Robert Padilla, a Field Application Scientist at Corning, dive into the topic of 3D culture techniques and why these technologies should be a part of any researcher’s repertoire. They will discuss the differences between spheroids and organoids, where they can be used, and the pros and cons of each culture type. They will also review customer case studies that exemplify the benefits of each culture type. And our speakers will offer tips for choosing the best models.

Spheroids vs. Organoids—A Data-Driven Approach for 3D Culture Model Selection

Sarah Dowdall, Director of Leadership and Strategy, will introduce Corning EcoChoice™ and explain how the program will benefit your environmental footprint. Learn how you can supply your lab sustainably.

This webinar covers:
-Introduction of the sustainable products program
-What makes a product more sustainable?
-How does this affect you?
-Where is this program headed in the future?

Introducing Corning EcoChoice™

三毛猫は雌ばかりですが、これはエピジェネティクスによる性染色体の調節が関係するため。クラウドファンディングによる支援で実施している私たちの三毛猫プロジェクトについてご紹介します。

三毛猫遺伝子探索プロジェクト

Scaling up adherent cell cultures is often a critical milestone as customers transition from research to clinical stages, but it's no easy feat. For adherent cell lines, this process can be very time-consuming, labor-intensive, and challenging to maintain consistent results over larger surfaces. Join our engaging webinar as we discuss the different strategies of scaling adherent cells with the various technology enablers from Corning Life Science, including the power of Corning HYPER technologies, fixed bed bioreactor and the application of microcarriers in enhancing cell culture scalability. Discover the interesting applications and case studies that demonstrate the incredible potential of these technologies.

The Adherent Cell Multiplier: Strategies for Scaling Success

エピジェネティクスは発生の過程で柔軟に遺伝子の働きを変える仕組みですが、発生が進むと細胞や個体の運命を決めてしまいます。哺乳類のエピジェネティクスと様々な生命現象の関わりについて解説します

エピジェネティクス：運命と偶然の科学

1. Exosomas; aplicaciones. Neil Vásquez (Universidad Nacional de Colombia)
2. Purificación y cuantificación de exosomas para aplicaciones de secretoma. Marío Camacho (Cryocell)

Terapias Celulares Avanzadas - Taller de Corning. Bloque 4

1. Atributos críticos de la calidad, marcadores, proliferación, potencia, diferenciación. Selem Torres (Instituto de Terapia Celular)
2. CRISPR-Cas y células CAR-T. Luis Daniel Hernández (Universidad de Guadalajara)

Terapias Celulares Avanzadas. Bloque 3

While often taken for granted, proper techniques for pipetting and pipettor handling are both very important when performing liquid handling measurements. Using proper technique is imperative for avoiding cross-contamination that can ruin the sample and affect the entire research process. By attending this webinar, you will learn about:

- Good pipetting practices: 2:41 - 5:54
- Proper pipetting techniques: 5:55 - 23:14
- Avoiding contamination: 23:15 - 29:24
- Key takeaways: 29:25 - 30:12 
- Q&A: 30:13 - 36:16

Pipetting Techniques and Avoiding Contamination

Pipettor handling

Pipetting procedures

Avoiding Contamination

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Pipetting Techniques and Avoiding Contamination

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