A major cost component of cell culture is fetal bovine serum (FBS), an often essential and very expensive. Many researchers routinely use media containing 10 to 20% FBS for growing the cells, but these levels may be higher than cells require. By reducing FBS levels by 50%, researchers may be able to save a considerable amount of money. Not all cell lines will do well at lower FBS levels although many will. This short presentation will help you better understand the steps you can take to reduce your cultures’ FBS levels and save money while keeping your cultures happy.