Name: 第2回 凍結細胞の解凍から継代まで
Start: 2022-09-13T06:00:00Z
End: 2022-09-13T06:01:00.000Z
Location: BrightTALK
Rating: 5

The Corning Scientific Seminar Series is a series of free, online technical presentations that provide novel tips, best practices and proven techniques to help advance your research. Delivered by scientists to scientists, these one-hour broadcasts offer useful information and tips for lab technicians and researchers.

2023年11月に実施した、九州大学佐々木先生と松田先生によるウェビナー、
【三毛猫遺伝子探索プロジェクト】の完結編として、
今年5月に発表されたオレンジ遺伝子の正体について再びお二方にお話しいただきます。

【三毛猫遺伝子探索プロジェクト】
三毛猫は雌ばかりですが、これはエピジェネティクスによる性染色体の調節が関係するためです。
この遺伝の仕組みが提唱されて60 年に渡り、謎に包まれていた三毛猫の毛色を作る遺伝子「オレンジ遺伝子」を解明することに成功しました。

三毛猫遺伝子探索プロジェクト-完結編-

This talk will highlight the importance of vendor selection, media storage, media usage, and contamination control strategies in efforts to guide scientists to find the right media formulations for their processes. With so many media vendors and options for media out there, how do you choose what is right for your current process and for your long-term process goals? Learn about key factors that can impact your decision when choosing a media vendor.

Cell Culture Media Quality Control Strategies

Understanding cellular organization in 3D systems enables the study of key biological processes such as cell–cell interactions and cell migration in a physiologically relevant context. These insights are critical for advancing personalized medicine and developing targeted therapies for diverse diseases.
In this webinar, we will explore Corning’s innovative portfolio for organoid generation, featuring advanced culture platforms and both biological and synthetic extracellular matrices designed to support next-generation organoid research.

Accelerating Organoid Discovery with Corning’s Advanced Technologies

What type of microplate to choose? The type of assay being performed will determine what kind of surface and/or material is required to generate the desired results. Corning offers microplates with a variety of modified surfaces and material combinations to support the (non-)binding or immobilization of cells, proteins, nucleic acids, and other biomolecules. In this webinar, we will discuss the different options and provide you with guidance in choosing the right microplate for your application.

Is it really just a microplate? The world of microplates revealed.

Learn about cutting-edge advancements in mesenchymal stromal cell (MSC) therapies for graft-versus-host disease (GvHD) and streamlined strategies for scaling up production.

Next-Generation MSC Therapies: Advancing Treatment for GvHD

Corning HYPERStack vessels provide an easy-to-use, space-saving solution for scaling up cell culture in research and production environments. Designed to support efficient, high-yield cell expansion, HYPERStack vessels have been used successfully across a variety of applications.

In this webinar, we will:

• Cover the essential design and operation principles of HYPERStack vessels, with practical tips to simplify use
• Discuss standout features and introduce new accessories to optimize your workflow
• Highlight real-world applications, including mesenchymal stem/stromal cells (MSCs) culture

Join us to explore how HYPERStack vessels can help you improve cell culture efficiency and achieve reliable results with confidence.

Efficient Cell Growth Made Simple with Corning® HYPERStack® Vessels

細胞培養では、どのような培養表面を選択するかはそれぞれの細胞や実験条件で異なってきます。コーニングは、その長い歴史の中で様々な細胞培養用表面を開発し続け、現在では20種を超える表面を有しています。今回のウェビナーでは、コーニングの種々の表面の特長を、日頃研究者の皆様からいただくご相談内容を踏まえた選択ポイントも交えてご紹介します。

最適な培養表面の選び方 ― コーニングの多様な細胞培養表面を徹底解説

This webinar will provide a comprehensive introduction to transepithelial/endothelial electrical resistance (TEER) and its critical role in evaluating the integrity and function of in vitro barrier models. Attendees will be introduced to WPI’s EVOM™ family of instruments, the industry standard in TEER measurement for over 35 years. The presentation will highlight how pairing EVOM systems with Corning Transwell Permeable Supports enable researchers to build robust epithelial and endothelial models, enhance data accuracy and consistency, and address common experimental challenges. During this presentation we will cover important aspects of the assay to consider during optimization in order to achieve consistent results.  We will also highlight both established and emerging applications of TEER across a range of research areas, including drug permeability, toxicity screening, and disease modeling.

Integrating World Precision Instrument’s EVOM™ TEER Technology with Corning® Transwell Permeable Supports

Laboratories have long been vital to innovation and discovery. Yet, their operations can carry a heavy environmental impact—largely due to resource-intensive processes and significant waste generation. As sustainability continues to take center stage for academic and industrial leaders alike, the need to measure and improve lab sustainability has never been more critical. During this webinar, we’ll discuss five key metrics to help you optimize your practices for sustainability. We’ll talk about energy usage, airflow management, emissions goals, waste, and water usage.

Gayatri (Gaya) Kasi has 8 years of product experience ranging from product engineering, product management, and now, product sustainability. Kasi has a bachelor's degree in Chemical Engineering from Worcester Polytechnic Institute and is currently on a path to get her MBA from Boston University with a concentration in Health Sector Management. In her current role as a Product Sustainability Portfolio Manager for Corning Life Sciences, she utilizes her engineering and business background to view sustainability from a design perspective to overcome challenges with single use plastics. Her mission is to innovate and drive adoption of sustainable products and circular solutions while maintaining product performance and quality as the most critical attributes for lifesaving research and production.

Measuring Sustainability in the Lab: 5 Metrics for Success

Three-dimensional (3D) cell culture offers a more physiologically relevant model than traditional 2D models, enabling deeper insights into cell behavior, disease modeling, and drug discovery. Achieving reliable results requires precise handling, well-planned workflows and careful matrix selection.
In this webinar we will:
- Work though the protocol with best practices 
- Discuss the Practical tips for working with Corning Matrigel and Synthegel
- Troubleshooting for Matrigel preparation, plating, and gel handling
- Selection the right Matrigel and Model for your experiment.

Optimizing 3D Cell Culture Workflows: Tips, Tricks, and Insights

In the era of gene therapy, mRNA vaccines and cell based therapeutics, demand for viral vectors is rapidly growing.  However, achieving high-titer, scalable and regulatory compliant viral production remains a major challenge for many manufactures.
This webinar will explore how Corning’s comprehensive portfolio-ranging from high-density cell culture system like CellSTACK and HYPERStack, to closed system and advanced surfaces- can streamline viral vector production at every step.
Whether you’re scaling from R&D to GMP or looking to boost production efficiency this session will provide actionable insights to help you go viral- the right way.

“More Vectors, Less Stress” - Simplifying Viral Production with Corning

Flexibility and scalability are essential for meeting the growing demands of cell culture in research, bioproduction, and cell therapy development. The Corning® CellCube® system offers a robust solution that can be tailored to support a wide range of cell types and workflows. 

In this webinar, we will:
• Highlight system features, demonstrating how protocols can be adjusted to suit specific cell types and applications.
• Explore the ease of setup, starting with the incubator-based configuration for initial feasibility assessments without capital investment.
• Showcase how to tailor the system including coating protocols, multiple seeding methods, and scalable configurations.

Join us to learn how the CellCube system’s flexibility and scalability can help you achieve consistent, high-quality results in your adherent cell culture applications.

Dr. Ann Rossi Bilodeau earned her Ph.D. in Pharmacology from the University of Rochester School of Medicine and Dentistry and completed postdoctoral training at the University of Chicago. A career scientist, Dr. Rossi Bilodeau has a strong foundation in physiology and drug discovery, with a research background that spans both academic and industry settings. Since joining Corning in 2017, she has developed deep expertise in Corning Life Sciences’ Bioprocess portfolio, with particular depth in Corning CellSTACK® and Corning CellCube platforms. Currently, Dr. Rossi Bilodeau is the Senior Bioprocess Applications Scientist, functioning as the technical lead for generating applications across the full range of Corning bioprocess technologies.

Test, Tailor and Scale: Unlocking the Flexibility of the Corning® CellCube® System

Serum Origin, Traceability, and Economics: Ensuring confidence in source through Corning's rigorous systems designed to safeguard your supply

The origin of serum is vital for customers in research, biomanufacturing, and scientific fields as it directly impacts experimental consistency, safety, and regulatory compliance. Trusted serum sources ensure reproducible results, minimize risks of contamination, and adhere to ethical and legal standards. Furthermore, transparency and traceability in serum origin provide customers with confidence in its quality and suitability for specific applications.

Mark Koza serves as Business Director for Media & Serum at Corning Life Sciences, where he has spent his two-decade career. He is responsible for strategy and execution of Corning's global serum portfolio, which includes all stages in the supply chain from supply to customer. 

Over the years at Corning Life Sciences, Mark has held various roles in commercial and business operations. He also serves as board chair with the International Serum Industry Association (ISIA).

Serum Origin, Traceability, and Economics

演題1　均一なスフェロイドの大量培養を実現するCorning® Elplasia® テクノロジー Corning Elplasia テクノロジーは、独自のマイクロキャビティ技術で、足場なしで大量のスフェロイドを形成することができます。多くの細胞タイプに適合し、薬物スクリーニング、がん/腫瘍生物学、幹細胞生物学、細胞治療研究、3D組織工学など、多くのアプリケーションに対応しています。本セミナーではT75フラスコと同様のフットプリントのCorning Elplasia 12Kフラスコと、ANSI/SLAS規格に準拠したCorning Elplasia 12Kオープンウェルプレートに加え、次期新製品もご紹介いたします。 演題2　FlowCam：フローイメージング法によるiPSC 3D培養球の形態解析と均一性評価 iPSCの3D培養では、細胞の均一性と拡張性を適切に評価することが不可欠です。FlowCamはフローイメージング法を採用し、数千のiPSCの形態や、サイズ分布を解析し、細胞培養状況を正確に把握することが可能です。最新のフローイメージング技術を駆使した新たなiPSC品質評価解析手法をご紹介いたします。

YOKOGAWA & Corning 共同セミナー

Join us on July 10, 2025, for a live webinar presented by Jesus Martinez, Laboratory Glass Product Line Manager at Corning Life Sciences. During this presentation we will discuss:
- Low actinic products
- Unique advantages of PYREX® Low Actinic
- The process behind creating low actinic laboratory glassware

Jesús Martínez is the Product Line Manager for the glass business at Corning Life Sciences - PYREX brand. A graduate in Industrial Engineering and Administration from the Autonomous University of Nuevo Leon, Jesús has accumulated eight years of experience in the industry, covering areas such as supply chain, quality, and business operations.  
Throughout his career, he has worked closely with PYREX labware products, understanding their quality and supply, as well as the engineering behind them. This experience has given him a comprehensive and in-depth view of the brand. His goal is to share the PYREX story with the public, highlighting the legacy and innovations of its products, and demonstrating how they can offer effective solutions in various contexts.

PYREX® Low Actinic Laboratory Glassware: Lights Off, Quality On

The field of stem cell therapy has undergone remarkable and dynamic advancements, expanding its applications to address a wide range of target diseases, including ischemic conditions, spinal cord injuries, cartilage defects, and even respiratory infections such as COVID-19. This progress has been accompanied by diversification in cell sources, encompassing both autologous and allogeneic cells, as well as adult stem cells and pluripotent stem cells, such as induced pluripotent stem cells (iPSCs). 
As numerous investigator-initiated clinical trials aim to develop innovative therapies and advance through IND filings and regulatory approvals, researchers often face significant challenges navigating the complex regulatory landscapes of their respective authorities. Corning provides tailored solutions to help researchers meet these stringent requirements, including support for regulatory compliance, optimized resource efficiency, product consistency, and automation. 
With the success story of the Ottawa Hospital in Canada serving as a testament, Corning is committed to empowering researchers who are striving to push the boundaries of scientific discovery and address critical unmet clinical needs. Together, we aim to transform possibilities into realities and drive impactful advancements in the field of regenerative medicine.

Shoot for the Stars: Overcoming GMP Hurdles in Stem Cell Therapy with Corning

This webinar will briefly introduce MSCs in the context of cell therapy and will then show the scale-up process used for the production of this cell type. This will include from the small scale in culture flasks used during preclinical research to the large-scale production for clinical trials using the Corning® HYPERFlask® in GMP production.

Corning HYPER Technology – A Cell Culture Scale-up Solution for Cell Therapy Processes

In this webinar we will discuss ways to achieve more consistent and better-quality results from your cells. We’ll cover concepts like reducing contamination, choosing the best surfaces for your cells, scaling up cultures and the importance of quality cell banking. 

Hilary Sherman is a Senior Scientist in the Corning Life Sciences Applications Lab located in Kennebunk, ME. Hilary has been with Corning since 2005 and has worked with a wide variety of cell types including mammalian, insect, primary, stem cells and organoids in a vast array of applications. Her key roles at Corning involve creating technical documents such as protocols and whitepapers, as well as providing technical support and training for both the Corning sales force and customers. Hilary received her B.S. degree in Biology from the University of New Hampshire.

Cell Culture Fundamentals: Best Practices for Better Cell Culture

実際の細胞培養に必要な凍結保存細胞の解凍・播種、継代までの一連の操作について、デモ動画を用いて解説します

第2回　凍結細胞の解凍から継代まで

細胞培養

凍結

細胞

回答

播種

継代

手技

無菌

融解

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第2回　凍結細胞の解凍から継代まで

Presented by

About this talk

Corning Scientific Seminar Series

第2回 凍結細胞の解凍から継代まで

Presented by

About this talk

Corning Scientific Seminar Series

第2回　凍結細胞の解凍から継代まで