Name: Bringing Gene Therapies to Life: Navigating Scale Up, Partnerships, and Barriers
Start: 2022-07-28T17:44:00Z
End: 2022-07-28T18:12:11.000Z
Location: BrightTALK
Rating: 0

The Corning Scientific Seminar Series is a series of free, online technical presentations that provide novel tips, best practices and proven techniques to help advance your research. Delivered by scientists to scientists, these one-hour broadcasts offer useful information and tips for lab technicians and researchers.

Extracellular matrix (ECM) can mimic in vivo environments for 2D and 3D cell culture applications. Corning Matrigel Matrix has been utilized as a powerful tool in general cell culture, assays, in vivo applications, stem cell culture and organoid culture. This webinar will introduce basic information on Corning Matrigel matrix and discuss tips and techniques for handling Corning Matrigel matrix. 

In this webinar, Kyung-A Song will review:
• Introduction to Corning Matrigel matrix
• Key features & material properties of Corning Matrigel matrix
• Usages of Corning Matrigel matrix
• Tips & techniques for handling Corning Matrigel matrix

Basics of Corning® Matrigel® Matrix

これから3D細胞培養を始める方向けに、知っておくべき3D細胞培養の基礎知識と実験に必要な材料について説明します。また、目的に合った3D細胞培養手法の選び方やベストプラクティスをコーニング製品を用いたアプリケーション情報を交えて解説します。今年発売したCorning® Matribot® バイオプリンターについてもご紹介します。

＜セミナーのポイント＞
・目的に応じた3D細胞培養手法の選び方
・すぐに役立つ3D細胞培養のベストプラクティス
・スフェロイドモデルを始める際の培養ポイント
・オルガノイドモデルを始める際の培養ポイント
・創薬・毒性学研究に活用され始めた3Dバイオプリンティング

3D細胞培養の始め方 ～3Dモデルシステムとコーニングが提供するソリューション～

In this webinar, we will introduce Corning®Matribot®, the first benchtop bioprinter designed to handle temperature-sensitive hydrogels. 
We will provide a comprehensive overview of the Matribot®’s features and capabilities, including its unparalleled Matrigel® dispensing and bioprinting functions. We will delve into the device’s precise dispensing function, enabling the formation of organoids, and its biopriting function for the creation of 3D skin models. In this webinar, attendees can expect to have a deep understanding of the Matribot® and to gain invaluable insights into its potential applications.

Introducing Corning® Matribot® Bioprinter for Organoid and Skin Model Formation

GreenLabs Recycling offers a solution for non-hazardous plastic currently going to landfills. This webinar will cover some statistics about non-hazardous waste in laboratories and our current and future solutions.

You will learn:
- How did GreenLabs Start and what do we do today? 
- What does the future look like?

Lab Plastic Recycling in the Greater Boston Area

There is intense interest in research applications of organoids—mini-organs grown in a dish that are made up of different cell types that self-organize into tissue assemblies. By accurately mimicking human tissue biology, organoids hold great promise to revolutionize scientific discovery and healthcare with multimodal potential for new drug development, precision medicine, and regenerative medicine. Human organoids can be derived from either human pluripotent stem cells (hPSCs) or primary biopsy or autopsy tissues. However, there are significant challenges in quality control and reproducibility.

In this webinar, two experts in 3D cell culture and organoid biology will share their expertise on this exciting field. Dr. Elizabeth Abraham (Corning Life Sciences) will discuss the latest developments in 3D cell culture, including key applications, new tools and technologies, and bridging the pre-clinical gap. The main presentation will be given by Dr. Lauretta Lacko (Weill Cornell Medicine), who will discuss the myriad medical applications of organoids. She will draw on her insights running the Human Therapeutic Organoid Core (HTOC) facility, providing quality organoids to the scientific community, in particular the value of sourcing high-quality reagents and optimizing protocols.

Accelerating Organoid-Based Scientific Discovery

Making sustainable and environmentally-friendly choices at home, from the products we buy to the behaviors we practice, is easy. However, once we enter the laboratory, we tend to forget some of these choices.

This webinar will review some best practices to help make your laboratory space more sustainable. Moving toward a more environmentally-friendly lab can include reducing energy usage, choosing consumables that minimize waste, and optimizing lab processes.

We will share tips, tricks, and unexpected places where you can make your laboratory a safe, healthy, and environmentally-conscious place for everyone. Any small change to your daily practices, in the lab and at home, can make a difference to the environment, and if you can foster a culture of sustainability, the impact will be even greater. It’s time to start CELLebrating any victory toward a better planet!

CELLebrate Sustainability in the Lab

It is very important to use proper pipetting techniques when measuring and transferring liquids for your applications in the laboratory. In addition, quick and precise pipetting will be necessary for reliable results and successful laboratory work.

This webinar will review the following:
- Good pipetting practices
- Proper pipetting techniques
- Avoiding contamination
- Key takeaways

Proper Pipetting Techniques

Cell and gene therapies are typically developed using cells grown in a small-scale format, such as a well plate or T-flask. To bring such therapies through the process development, clinical trial, and commercial manufacturing phases, it is necessary to produce a progressively larger number of cells.

Cell cultures can be scaled out by increasing the number of vessels, but this approach increases the processing time proportionally. For adherent cell cultures, the best solution is to scale up by increasing the cell growth surface area of each vessel, which offers reduced processing time per million cells.

This seminar will explore several technologies for the initial scale up of adherent cell cultures and review considerations for subsequent scale up.

A Beginner’s Guide to Scaling Up Cells

This webinar will demonstrate the use of the Corning Matribot Bioprinter to accurately and consistently dispense pancreatic cancer organoids for drug testing. Dispensed organoid cultures were assessed for toxicity to several chemotherapeutics that are traditionally used for treating pancreatic cancer. This data demonstrates an automated way to screen for the best drug choice for an individual’s pancreatic cancer.

Corning® Matribot® Bioprinter for Organoid Assays

This March, Corning Life Sciences is celebrating our women leaders in STEM. As of 2019, women made up 38% of all STEM workers, and that number is on the rise. Hear from Elizabeth Abraham on her experiences starting a career as a woman in STEM, and how she has grown within it.

Growth and Diversity with Careers in STEM

In this webinar, we will detail regulated medical waste (RMW) management innovations including:
-A comparative review of traditional RMW management practices 
-An exciting, new, environmentally friendly treatment alternative for RMW with no hazardous emissions
-Opportunities for significant sustainability improvements in managing RMW, including reliable, safe, efficacious treatment and reclamation of targeted plastic constituents.

Ozone: The Future of Medical Waste Management and More?

3D cell culture allows you to more accurately model in vivo tissue under in vitro conditions. However, traditional techniques and workflows to generate these cultures can be tedious, inconsistent, and time consuming. This talk will highlight new tools available for researchers to help accelerate and scale their spheroid and organoid workflows, including the Corning® Matribot® bioprinter and Corning Elplasia® 12K flask.

3D Cell Culture Solutions to Maximize Biological Complexity and Throughput

Investigating the relative contribution of mutations, nutrient availability, and 2D vs. 3D growth on the behavior of cancer cells.

This webinar covers:
-Constructing a genotypic series of colon cancer organoids
-Characterizing  3D organoids vs. isogenic 2D cell lines
-Mutations, Media, and 2D vs. 3D. What determines the metabolic state of cancer cells?

Tutorial: Genes or Environment? Investigating the Behavior of Cancer Cells

A simplified approach for rapid analysis of thousands of intact spheroids using the Velocyt® LP Large Particle Flow Cytometer.

The Velocyt address the issues of 3D multicellular models: big objects, intact analysis, high sample numbers and total sample recovery. Using Corning® Elplasia® technology simplifies the Velocyt workflow and increases assay robustness and reproducibility.

Tutorial: Rapid Analysis of Intact Spheroids using the Velocyt® LP Cytometer

Throughout the medical community, there is a growing concern about the rising costs for bio-hazardous waste remediation. Organizations have begun to examine the effects on landfills, and the impacts of incineration, originating from this type of waste. Most organizations today are seeking more effective and new approaches to this critical process outside of the standard practice of removal and destruction of the bio-hazardous waste by a 3rd party vendor, but the options have been limited – until now. 

To combat this ever-expanding problem of RMW, Sterilis Solutions sought a solution that addressed all the impacts detailed above. The solution came in the form of their product, the Remediator. The Remediator, which is no bigger than a standard office copier, combines a steam sterilizer and grinder into a single unit. In a few simple steps, the biohazardous waste found in sharps containers and biohazard bags is reduced to what is essentially harmless confetti. It can then be entered into an advanced recycling program.

An Alternative Approach to Regulated Medical Waste (RMW) Hauling

Once thought to be merely evictees of cell debris, extracellular vesicles (EVs) hold the promise of the next generation therapeutic agent. EVs are known cell-to-cell messengers that mediate physiological functions throughout the body. Their ability to stealthily transfer paracrine factors, nucleic acids, proteins, and other essential biomolecules between distant tissues have the potential to usher in a new era of nanomedicine.

This webinar will focus on why stem cell derived EVs are a compelling acellular therapeutic. We will discuss the unique characteristics of EVs compared to other synthetic therapeutic agents and outline the critical attributes necessary to be a successful regenerative medicine alternative to whole stem cell therapies. We will explore how naïve EVs are produced, isolated, and characterized with an emphasis on emerging approaches in characterization and quantification methods. Lastly, we will review the application of EVs as natural therapeutic agents in the clinic, which include regenerative medicine, clinical oncology, and COVID-19 therapies.

Extracellular Vesicles: From Waste EVictees to Therapeutic Reality

Cell culture media is one of the most important components of the in vitro tissue environment. It provides the nutrients, minerals and growth factors necessary to sustain cells outside the body.

This Teach Me in 10 explores the different media options available for plating mammalian cells. It covers preparation techniques, storage options and how to choose the right media for your experiment.

Plating Cells: Preparation and Media Options With Ann Rossi Bilodeau

The field of gene and cell therapy has experienced rapid growth over the past decade, and the expansion of the number of therapeutics coincides with the growth of the scale of manufacturing. Through the seed train process, manufacturing begins with a few million cells and ultimately produces billions of cells to serve patient populations. With so much material on the line, researchers face the task of minimizing risks and maximizing product quality. Closed systems in conjunction with bioreactor systems provide a controlled and monitored environment that can not only enhance cell performance, but also provide a scalable path from process development to manufacturing. This seminar will review adherent cell culture scale up with attention to the following topics:
- Considerations for seed train optimization 
- Considerations for closed system process development and manufacturing
- Adherent cell culture bioreactor systems from Corning, including the Ascent® FBR

Amping Up Yield and Enhancing Quality in Adherent Cell Culture Scale-Up

Cell and gene therapies are rapidly advancing and with that, the need to effectively generate large volumes of viable cell and gene therapy products to fuel the pipeline. In this fast-paced industry, researchers, manufacturers, life science companies, and academics are working together to solve the significant challenges. Join Corning and Expression Therapeutics in a discussion about their experiences transitioning from R&D to upstream manufacturing for gene therapies: navigating many variables and overcoming obstacles to innovate the process and make novel gene therapies a reality. 

This discussion will cover:
- Key challenges and considerations for moving gene therapies into clinical trials
- How to overcome challenges in manufacturing with creative thinking to push technologies forward
- How industry partnerships are evolving and how you can leverage them to drive innovation

Roundtable Guests: 
William Swaney, M.S., President, Expression Manufacturing LLC
Gabriela Denning, Ph.D., Vice President of Research & Development, Expression Therapeutics 
Anindya Dasgupta, Ph.D., Director GMP Manufacturing, Expression Manufacturing LLC

Bringing Gene Therapies to Life: Navigating Scale Up, Partnerships, and Barriers

cell therapy

gene therapy

cell and gene therapy

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Bringing Gene Therapies to Life: Navigating Scale Up, Partnerships, and Barriers

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