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Tips and Techniques for Enhancing Your Cell Culture

Advances in the understanding of the behavior and function of biological systems are dependent on the study of cells and tissues. An essential tool in this research is the use of in vitro cell culture. Many variables contribute to providing a physiological environment for the cell in the laboratory, a few examples include cell source, isolation techniques, growth conditions such as matrix proteins and soluble factors, and cell age. Basic laboratory practices are sometimes overlooked as a source of discrepancy in data; however the application of fastidious and reproducible technique can reduce cell culture as a source of data variation. As specialized techniques have been developed to modulate cells and tissues in vitro, the importance of reproducible data has become paramount. In this presentation, we will identify and discuss basic principles of in vitro mammalian cell culture that influence the quality of experimental results.
Recorded May 14 2013 61 mins
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Presented by
Paula M. Flaherty
Presentation preview: Tips and Techniques for Enhancing Your Cell Culture

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    Tim Baranowski, Ph.D.,Field Applications Scientist at Molecular Devices and Erin Edwards, Ph.D., Head of Services at Visiko
    For this webinar, we have partnered with Molecular Devices and Visikol to show how integrating novel 3D cell culture, tissue clearing, and high-content imaging techniques can better identify therapeutic solutions to NASH/NAFLD.


    Learn how to:
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    •Apply tissue clearing techniques to fully characterize 3D cell culture models in a single workflow
    •Utilize water immersion objectives and targeted acquisition with confocal imaging to fully characterize 3D fibrosis models in a high throughput manner
    •Perform 3D volumetric analysis and other custom analyses for unique feature extraction and quantification
  • 3D Cell Cultures and Tissue Clearing Recorded: Nov 25 2020 51 mins
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    There are many important considerations for how best to image a 3D cell culture model. Moreover, there are numerous high content imaging systems available. This webinar reviews some of the systems available,best practices when imaging 3D cell culture models, and how to employ tissue clearing for improved imaging. Additionally, the presenters share several case studies in which tissue clearing and high content confocal microscopy are integral to addressing specific research questions:
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    • Dipping vs. air objectives
    • Basic principles of confocal microscopy
    • Best practices for labeling 3D cell culture models
    • Wide field vs. confocal imaging
    • 3D image analysis

    Webinar Highlights:
    - Introduction: 00:00 - 3:18
    - The State of 3D Cell Culture: 3:19 - 8:19
    - Tissue Clearing and Labeling Techniques for 3D-CCM: 8:20 - 17:20
    - High Content Imaging Considerations: 17:21 - 28:07
    - Workflow and Case Study - 3D Cell Culture Models for Immunoncology: 28:08 -38:14
    - Q&A Session: 38:15 - 51:48
  • COVID-19: Insights Through 3D Cell Culture Recorded: Nov 19 2020 53 mins
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  • SARS-CoV-2: Insights Through 3D Cell Culture Recorded: Oct 20 2020 58 mins
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    Speaker Bio:
    Mourad Ferhat, Ph.D.,
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  • Maximizing Cell Yields Using Perfusion Recorded: Oct 1 2020 35 mins
    Dr. Ann Rossi Bilodeau
    Progress in cell and gene therapy research will accelerate clinical cell therapy programs in the coming years. As cell therapy trials advance from pre-clinical to late-stage trials, cell culture platforms will need to meet increasing demands for cell yield.

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    00:00 - 01:30: Introduction
    1:31 - 7:50: Considerations and challenges for scaling adherent cell growth
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    14:08 - 24:30: Case Study - HEK293T and Vero Cell Expansion
    24:31 - 34:30: Q&A Session

    About the Presenter:
    Dr. Ann Rossi Bilodeau graduated from the University of Rochester School of Medicine and Dentistry with a Ph.D. in Pharmacology and received postdoctoral training at the University of Chicago. Ann began her career with Corning Life Sciences as the Applications Lab Manager where she utilized her strong academic and industry research experience to direct the activities of the Applications Laboratory. Currently, Dr. Rossi Bilodeau is the Senior Bioprocess Applications Scientist, functioning as the technical lead for generating applications in support of Corning Life Sciences’ Bioprocess and Cell/Gene Therapy portfolios including CellCube and HYPERStack platforms.
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    About the presenter:
    Dr. Francesco Boccellato studied at Sapienza University of Rome. Ever since he was a student, he's been interested in the impact of pathogens in causing cancer in humans. During his Ph.D. in Rome, he studied the role of the Epstein - Barr virus (EBV) in deregulating microRNAs in diffuse large B cell lymphomas. After his Ph.D., Francesco moved to Berlin to the Max Planck Institute for Infection Biology, were he became interested in the causality of bacterial infection in human cancers. To study the impact of infection on healthy epithelial barriers Francesco invented a new cultivation systems for healthy epithelial cells called the mucosoids. The mucosoids are monolayers of polarised epithelial cells that recapitulate all the functions of an epithelial barrier in the body, including mucus production. Francesco recently started his independent group in the University of Oxford (UK) at the Ludwig Institute for Cancer Research.
  • 3D Cell Cultures and Tissue Clearing Recorded: Sep 24 2020 52 mins
    Ian MacCloud, Laboratory Manager at Visikol Inc. and Dr. Ann Rossi Bilodeau, Corning Life Sciences
    There are many important considerations for how best to image a 3D cell culture model. Moreover, there are numerous high content imaging systems available. This webinar reviews some of the systems available,best practices when imaging 3D cell culture models, and how to employ tissue clearing for improved imaging. Additionally, the presenters share several case studies in which tissue clearing and high content confocal microscopy are integral to addressing specific research questions:
    • LED vs. laser illumination
    • Dipping vs. air objectives
    • Basic principles of confocal microscopy
    • Best practices for labeling 3D cell culture models
    • Wide field vs. confocal imaging
    • 3D image analysis

    Webinar Highlights:
    - Introduction: 00:00 - 3:18
    - The State of 3D Cell Culture: 3:19 - 8:19
    - Tissue Clearing and Labeling Techniques for 3D-CCM: 8:20 - 17:20
    - High Content Imaging Considerations: 17:21 - 28:07
    - Workflow and Case Study - 3D Cell Culture Models for Immunoncology: 28:08 -38:14
    - Q&A Session: 38:15 - 51:48
  • Qualidade e Acreditação em Laboratorios - Uma abordagem da influência do vidro Recorded: Sep 17 2020 76 mins
    Jerónimo Figueiredo
    Qualidade e Acreditação em Laboratorios - Uma abordagem da influência da vidraria
    Jerónimo Figueiredo - Business Operations Manager
  • HUB Organoids™: Impacting Clinical Outcomes Using Organoid Disease Models Recorded: Sep 17 2020 45 mins
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    Learn how our guest presenter is using organoid models to predict patient response and provide access to patients to treatments for Cystic Fibrosis.
    This webinar covers:
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    Webinar highlights:
    - Introduction: 00:00 - 2:23
    - HUB Organoids - Adult Stem Cell-Derived Organoids: 2:24 - 7:55
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    - Oncology and HUB Tumor Organoid Models: 21:17 - 30:18
    - Q&A Session: 30:19 - 44:11
  • Segurança e Qualidade em Laboratórios - Impacto da vidraria Recorded: Sep 16 2020 91 mins
    Jerónimo Figueiredo - Business Operations Manager
    Segurança e Qualidade em Laboratórios - Impacto da vidraria
    Jerónimo Figueiredo - Business Operations Manager
  • Organogenesis to Oncogenesis Recorded: Sep 15 2020 46 mins
    Dr. Joseph Regan
    Organogenesis to Oncogenesis: Modeling Development and Cancer with Organoids
    Organoid cell culture allows mammalian stem cells to differentiate and self-organize into three-dimensional organ-like tissues that reflect the key structural and functional properties of the organs from which they are derived. In addition, organoids can be grown from patient-derived healthy and tumor tissue to provide genetically and phenotypically stable personalized preclinical models. Organoid technologies therefore provide a unique in vitro system in which to study human organ development and cancer.

    In this webinar, Dr. Regan will discuss the use of organoid technologies to study organogenesis and cell fate determination in the postnatal mammary gland. In addition, the use of patient-derived organoids (PDOs) to elucidate the molecular mechanisms that regulate oncogenesis and contribute to tumor heterogeneity in colon cancer will be discussed.

    Presenter:
    Dr. Joseph Regan graduated from The Institute of Cancer Research (University of London, UK) with a Ph.D. in Cell Biology and received postdoctoral training at the Breast Cancer Now Research Centre (London, UK) and the Max Planck Institute for Molecular Genetics (Berlin, Germany). Dr. Regan subsequently worked as a Research Scientist with Bayer AG and the Charité Medical University (Berlin, Germany), where he uses patient-derived organoid models to study cancer stem cells and tumor heterogeneity in colon cancer.
  • 最新バイオロジクス技術を用いた細胞・遺伝子治療開発の海外導入事例を紹介 Recorded: Sep 8 2020 60 mins
    コーニングインターナショナル株式会社  ライサイエンス事業部 石渡 孝至
    欧米を中心に実用化が加速し始めた細胞・遺伝子治療分野の幹細胞やウイルスベクターの産業化に向け、コーニング独自のハイパーテクノロジー(HYPERFlask®、HYPERStack®)を中心にクローズドシステムによる無菌操作法や最終製剤化の安全操作性について概説いたします。
  • Generando un Microambiente Óptimo en Todas las Etapas del Cultivo Celular Recorded: Aug 28 2020 26 mins
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    Corning y Aplicaciones en Bioprocesos: Medio de Cultivo y Suero - Parte 3/3

    El medio de cultivo y suero son componentes esenciales del cultivo celular – suministrando nutrientes y factores claves para las células – en ese sentido, seleccionar los productos óptimos es esencial.
    En este Webinar presentaremos una revisión general del porfolio de medio y suero de Corning, y como estos productos pueden ser usados en aplicaciones de bioprocesos para realzar tanto procesos de producción e investigación.
    El portfolio de Corning incluye:
    -Diversas formulaciones de medio estándar para requerimientos específicos
    -Medio libre de suero y medios especializados
    -Suero estándar y premium
    -Selección de suero especializado.

    Biografía del Expositor
    Luego de graduarse como Bioquímico de la Universidad de Santiago de Chile, Constanza prosiguió su carrera en la industria farmacéutica veterinaria, desempeñándose como Científico de Bioprocesos tanto para el desarrollo de vacunas bacterianas y virales, estando a cargo de los procesos de escalado y generación de antígenos. Luego continuo su carrera en Bélgica, esta vez en el área de la producción de anticuerpos y proteínas recombinantes en sistemas celulares en suspensión, generando optimización de protocolos y experimentos de escalado.
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    Los procesos en suspensión juegan un rol clave en el mercado de biofarmacéuticos, donde sistemas celulares en suspensión son usados para producir anticuerpos y proteínas recombinantes. En este contexto, la selección de las plataformas de cultivo adecuadas favorece el uso eficiente del espacio, reduce costos y horas de trabajo. En este Webinar haremos una revisión general del portfolio de Corning para el cultivo en suspensión, aplicado tanto al ámbito de la investigación y desarrollo y producción. Apoyado además con datos experimentales generados por nuestros equipos de desarrollo.

    Biografía del Expositor
    Luego de graduarse como Bioquímico de la Universidad de Santiago de Chile, Constanza prosiguió su carrera en la industria farmacéutica veterinaria, desempeñándose como Científico de Bioprocesos tanto para el desarrollo de vacunas bacterianas y virales, estando a cargo de los procesos de escalado y generación de antígenos. Luego continuo su carrera en Bélgica, esta vez en el área de la producción de anticuerpos y proteínas recombinantes en sistemas celulares en suspensión, generando optimización de protocolos y experimentos de escalado.
  • Corning HYPER Technologies: Compact Solutions for Cell Scale-Up [ENG. VERSION] Recorded: Aug 27 2020 35 mins
    Dr. Lindsey Parker
    Efficient cell scale-up is essential in biologics and vaccine production to facilitate optimal product yields, whilst reducing footprint and manhours, Corning introduces the HYPER vessel technology. Corning HYPERFlasks and HYPERStacks are multilayer vessels incorporating proprietary gas-permeable film technology, resulting in high surface area to volume ratio. Using HYPER vessels, scientists can generate greater yields than traditional cell culture technologies, saving time, space and labor.

    In this webinar we will provide a general introduction of the HYPER technology, as well as technical applications in the fields of cell and gene therapy and viral particle production.

    About presenters:

    Dr. Lindsey Parker
    Lindsey completed her Ph.D. in Tissue Engineering and Regenerative Medicine at the University of Leeds (UK), validating a bioreactor for the application of mechanical stimuli to MSC. She subsequently joined UCL as an industrial KTP associate, leading clinical research in umbilical cord- derived MSC scale-up in collaboration with a tissue bank. Lindsey is currently a Bioprocess Sales Specialist at Corning, providing specialist support in bioprocess ranges, such as advising on product selection, custom products, and providing training.
  • Corning HYPER Technologies: Compact Solutions for Cell Scale-Up [SP. VERSION] Recorded: Aug 27 2020 28 mins
    Constanza Curotto
    Efficient cell scale-up is essential in biologics and vaccine production to facilitate optimal product yields, whilst reducing footprint and manhours, Corning introduces the HYPER vessel technology. Corning HYPERFlasks and HYPERStacks are multilayer vessels incorporating proprietary gas-permeable film technology, resulting in high surface area to volume ratio. Using HYPER vessels, scientists can generate greater yields than traditional cell culture technologies, saving time, space and labor.

    In this webinar we will provide a general introduction of the HYPER technology, as well as technical applications in the fields of cell and gene therapy and viral particle production.
    About presenter:

    Constanza Curotto
    After graduating from Biochemistry in the University of Santiago (Chile), Constanza pursued directly a career in the Chilean Vaccine R&D industry, developing as a bioprocess scientist for both bacterial and viral vaccines in the field of animal health, being in charge of scale-up, and tech transfer with manufacturing plants. She furthered developed her career in Belgium as a bioprocess scientist, this time in the field of recombinant protein production, pursuing protocol optimization and scale-up experiments with suspension mammalian cell lines. Constanza is currently a field applications scientist in Corning for the EMEA region, providing customers with in-depth technical support, presenting seminars and trainings, and assisting customers in experimental set-up and troubleshooting.
  • Corning HYPER Technologies: Compact Solutions for Cell Scale-Up [FR. VERSION] Recorded: Aug 27 2020 48 mins
    Dr. Sonia Meconi
    Efficient cell scale-up is essential in biologics and vaccine production to facilitate optimal product yields, whilst reducing footprint and manhours, Corning introduces the HYPER vessel technology. Corning HYPERFlasks and HYPERStacks are multilayer vessels incorporating proprietary gas-permeable film technology, resulting in high surface area to volume ratio. Using HYPER vessels, scientists can generate greater yields than traditional cell culture technologies, saving time, space and labor.

    In this webinar we will provide a general introduction of the HYPER technology, as well as technical applications in the fields of cell and gene therapy and viral particle production.

    About presenters:

    Dr. Sonia Meconi
    Sonia’s scientific background covers different fields such as biochemistry, cell biology, and immunology where she obtained a Ph.D., specifically in human infectious diseases working on a daily basis in a biologic level 3 lab. After a post-doctoral fellow and lecturer experience, she took over the head of the R&D and production teams in a small biotech which developed human serological diagnosis assays for infectious and autoimmune diseases. She oversaw pathogens (bacteria and virus) expansion supervision for protein microarray tests. Then, she joined an international company as an Application Specialist for cell culture and purification, creating new cell culture media dedicated to vaccines and cell therapy markets. Now, she joined Corning as Bioprocess Specialist working for different suppliers for the biopharmaceutical industry.
Training for Life Science Researchers
The Corning Scientific Seminar Series is a series of free, online technical presentations that provide novel tips, best practices and proven techniques to help advance your research. Delivered by scientists to scientists, these one-hour broadcasts offer useful information and tips for lab technicians and researchers.

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  • Title: Tips and Techniques for Enhancing Your Cell Culture
  • Live at: May 14 2013 4:00 pm
  • Presented by: Paula M. Flaherty
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